Author: M. H. F. Wilkinson
Publisher: John Wiley & Sons
ISBN: 9780471974406
Category : Science
Languages : en
Pages : 588
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Book Description
Providing specific knowledge in the theory of image analysis, optics, fluorescence, and imaging devices in biomedical laboratories, this timely and indispensable volume focuses on the theory and applications of detection, morphometry, and motility measurement techniques applied to bacteria, fungi, yeasts and protozoa.
Author: Tor Savidge
Publisher: Elsevier
ISBN: 9780125215350
Category : Science
Languages : en
Pages : 420
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Book Description
Recent advances in molecular technology have provided new microbial imaging tools, not only complementing more classical methods, but in many cases significantly enhancing the sensitivity and efficiency in which studies may be conducted. These technologies are applicable to a wide range of problems in contemporary microbiology, including strain selection, understanding microbial structure, function and pathophysiology, as well as in the development of anti-microbial agents and vaccines. This volume emphasizes detailed methodology, provides a theoretical background and lists potential applications of specific imaging tools. * Edited by two experts in the field * Applicable to a broad Microbiology readership * Highly illustrated * Provides in-depth accounts from scientists working with cutting edge technologies * Facilitates researchers who involve Microbial Imaging in their work
Author: William A. Siebold
Publisher:
ISBN:
Category : Bacteria
Languages : en
Pages : 158
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Book Description
The most abundant clone found in ribosomal RNA clone libraries obtained from the world's oceans belongs to the SAR11 phylogenetic group of environmental marine bacteria. Imaging and counting SAR11 bacterial cells in situ has been an important research objective for the past decade. This objective has been especially challenging due to the extremely small size, and hypothetically, the low abundance of ribosomes contained by the cells. To facilitate the imaging of small dim oligotrophic bacterial cells, digital imaging technology featuring very small pixel size, high quantum yield scientific grade CCD chips was integrated with the use of multiple oligonucleotide probes on cells mounted on a non-fluorescing solid substrate. Research into the composition of bacterioplankton populations in natural marine systems follows a two-fold path. Increasing the culturability of microbes found in the natural environment is one research path. Identifying and enumerating the relative fractions of microorganisms in situ by culture-independent methods is another. The accumulation and systematic comparison of ribosomal RNA clones from the marine environment has resulted in a philosophical shift in marine microbiology away from dependence upon cultured strains and toward investigations of in situ molecular signals. The design and use of oligonucleotide DNA probes targeting rRNA targets has matured along with the growth in size and complexity of the public sequence databases. Hybridizing a fluorescently labeled oligonucleotide probe to an rRNA target inside an intact cell provides both phylogenetic and morphological information (a technique called Fluorescence in situ Hybridization (FISH)). To facilitate the imaging of small, dim oligotrophic bacterial cells, digital imaging technology featuring very small pixel size, high quantum yield, scientific grade CCD chips is integrated with the use of multiple oligonucleotide probes on cells mounted on a non-fluorescing solid substrate. This research develops the protocols necessary to acquire and analyze digital images of marine bacterial cells. Experiments were conducted with Bermuda Atlantic Time Series (BATS) environmental samples obtained during cruise BV21 (1998) and B138 (2000). The behavior of the SAR114*Cy3 probe set when hybridized to bacterial cells from these samples was investigated to determine the optimal hybridization reaction conditions. The challenges of bacterial cell counting after cell transfer from PCTE membrane to treated microslides were addressed. Experiments with aged Oregon Coast seawater were performed to investigate the protocol used to transfer cells from membrane to microslides, and examined the distribution of cells and the statistics of counting cells using traditional epifluorescence microscopy and image analysis techniques.
Author: Thomas Mitchell Schmidt
Publisher: Academic Press
ISBN: 0123838789
Category : Science
Languages : en
Pages : 772
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Book Description
This book provides an overview of ecological aspects of the metabolism and behavior of microbes, microbial habitats, biogeochemical cycles, and biotechnology. It was designed by selecting relevant chapters from the comprehensive Encyclopedia of Microbiology, 3rd edn., and inviting the original authors to update their material to include key developments and advances in the field.
Author: Bernhard Sonnleitner
Publisher: Springer
ISBN: 3540487735
Category : Science
Languages : en
Pages : 257
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Book Description
With contributions by numerous experts
Author:
Publisher: Academic Press
ISBN: 0123739446
Category : Science
Languages : en
Pages : 4358
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Book Description
Available as an exclusive product with a limited print run, Encyclopedia of Microbiology, 3e, is a comprehensive survey of microbiology, edited by world-class researchers. Each article is written by an expert in that specific domain and includes a glossary, list of abbreviations, defining statement, introduction, further reading and cross-references to other related encyclopedia articles. Written at a level suitable for university undergraduates, the breadth and depth of coverage will appeal beyond undergraduates to professionals and academics in related fields. 16 separate areas of microbiology covered for breadth and depth of content Extensive use of figures, tables, and color illustrations and photographs Language is accessible for undergraduates, depth appropriate for scientists Links to original journal articles via Crossref 30% NEW articles and 4-color throughout – NEW!
Author: James Pawley
Publisher: Springer Science & Business Media
ISBN: 9780387259215
Category : Science
Languages : en
Pages : 1018
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Book Description
Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.
Author: Parashuram Bannigidad
Publisher:
ISBN: 9783659890321
Category :
Languages : en
Pages : 148
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Book Description
Author: Christon J. Hurst
Publisher: American Society for Microbiology Press
ISBN: 1555813798
Category : Science
Languages : en
Pages : 3023
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Book Description
The most definitive manual of microbes in air, water, and soil and their impact on human health and welfare. • Incorporates a summary of the latest methodology used to study the activity and fate of microorganisms in various environments. • Synthesizes the latest information on the assessment of microbial presence and microbial activity in natural and artificial environments. • Features a section on biotransformation and biodegradation. • Serves as an indispensable reference for environmental microbiologists, microbial ecologists, and environmental engineers, as well as those interested in human diseases, water and wastewater treatment, and biotechnology.
Author: Asoke K. Nandi
Publisher: Springer
ISBN: 9811083541
Category : Technology & Engineering
Languages : en
Pages : 411
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Book Description
This book comprises a collection of papers by international experts, presented at the International Conference on NextGen Electronic Technologies (ICNETS2-2017). ICNETS2 encompassed six symposia covering all aspects of electronics and communications engineering domains, including relevant nano/micro materials and devices. Featuring the latest research on computational signal processing and analysis, the book is useful to researchers, professionals, and students working in the core areas of electronics and their applications, especially signal processing, embedded systems, and networking.
