Development of Diagnostic Probes for Identification of Species and Races of Root-knot Nematode (Meloidogyne Spp.) PDF Download
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Author: J. M. Stanton
Publisher:
ISBN:
Category : Plant nematodes
Languages : en
Pages : 36
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Author: J. M. Stanton
Publisher:
ISBN:
Category : Plant nematodes
Languages : en
Pages : 36
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Author: Churamani Khanal
Publisher:
ISBN: 9781321376111
Category : Plant nematodes
Languages : en
Pages : 124
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Book Description
Root-knot nematodes (Meloidogyne spp.) are highly-adaptable, obligate plant parasites distributed worldwide. In addition, root-knot nematodes are an economically important genus of plant-parasitic nematodes. Meloidogyne incognita, M. arenaria, M. javanica, M. hapla and M. graminis have been reported from Arkansas during 1964 to 1994. Previous identifications were based primarily on morphological characters and host differentials. In this study, identification using molecular diagnostics methods was performed to identify Meloidogyne species present in Arkansas. A total of 106 soil and root samples from 36 of the 75 counties were collected and processed to obtain root-knot nematodes. Polymerase chain reaction (PCR) was performed to amplify a region between cytochrome oxidase II and 16s ribosomal mitochondrial DNA (mtDNA) genes of root-knot nematodes. Of the species identified in the present survey, M. incognita was the most abundant followed by a few isolated samples of M. marylandi, M. haplanaria, M. hapla, M. arenaria and M. partityla. As a result of the sequencing analysis, a new set of PCR primers that amplifies the same region of mtDNA was designed to identify M. incognita. Furthermore, a species specific PCR protocol was developed using mitochondrial marker for routine nematode identifications of the most common species, M. incognita. Additionally, cultures of different species identified were established and maintained in the greenhouse on a suitable host to facilitate future research. Since correct identification of species in the field is imperative for effective control of any pathogen, findings from this research will be useful in developing suitable crop management strategies in Arkansas.
Author: Albert Lee Taylor
Publisher:
ISBN:
Category : Nematode diseases of plants
Languages : en
Pages : 128
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Author: Susan B. Jepson
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 282
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Book Description
History of the genus Meloidogyne up to Chitwood; Classification of Meloidogyne; Species combinations recognized in the present work; Species of known economic importance; Symptioms of root-knot nematode damage; Problems associated with identification of Meloidogyne species; Aspects of control; The necessity for identification to species some examples; Hosts and distributions of Meloidogyne species; Preparation methods for culturing and identification; Character assessment and description of diferential characters for identification; Comparative morphology and descriptions of species; keys the identification of Meloidogyne species; Descriptions of two new Meloidogyne species.
Author:
Publisher:
ISBN:
Category : Nematode diseases of plants
Languages : en
Pages : 120
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Author: Franco Lamberti
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 504
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Author: Albert Lee Taylor
Publisher:
ISBN:
Category : Meloidogyne
Languages : en
Pages : 84
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Book Description
Een identificatiegids aan de hand van gedetailleerde tekeningen
Author: Roland N. Perry
Publisher: CABI
ISBN: 184593492X
Category : Technology & Engineering
Languages : en
Pages : 530
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Book Description
Root-knot nematodes are the most economically important group of plant-parasitic nematodes worldwide, and their control presents a major global challenge. Advances are being made in understanding their biology, host-parasite interaction and management strategies. Covers the taxonomy, classification, morphology, life-cycle biology, genomes, resistance, sampling, detection, and management strategies of these pests.
Author:
Publisher:
ISBN:
Category : Root-knot
Languages : en
Pages : 164
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Book Description
Author: Jonathan David Eisenback
Publisher:
ISBN:
Category : Meloidogyne
Languages : en
Pages : 60
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Book Description
