Development of Biosensors Based on DNA Aptamers for Direct Mycotoxins Detection PDF Download
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Author: Nawel Mejri
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
This aim of this work is to develop ultrasensitive electrochemical biosensors with high affinity toward ochratoxine (OTA) and aflatoxine M1 (AFM1). In order to obtain the best analytical performances, we associated nano-materials in the transducer construction: conducting polypyrrole polymer and poly(amido-amine) dendrimères. Thanks to this association, we benefited from the conducting material's electrical properties, and the large active detection surface dendrimers. For the bimolecular sensing part, we used specific DNA aptamers which allowed us to quantify mycotoxines at nM concentrations. In addition, the different aptamer based biosensors present a very large dynamic ranges. We also demonstrated through the use of different sizes of dendrimers, that the sensitivity depend not only in the affinity between bioreceptors and their target molecules, but also in the physico-chemical properties of the biosensor.
Author: Nawel Mejri
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
This aim of this work is to develop ultrasensitive electrochemical biosensors with high affinity toward ochratoxine (OTA) and aflatoxine M1 (AFM1). In order to obtain the best analytical performances, we associated nano-materials in the transducer construction: conducting polypyrrole polymer and poly(amido-amine) dendrimères. Thanks to this association, we benefited from the conducting material's electrical properties, and the large active detection surface dendrimers. For the bimolecular sensing part, we used specific DNA aptamers which allowed us to quantify mycotoxines at nM concentrations. In addition, the different aptamer based biosensors present a very large dynamic ranges. We also demonstrated through the use of different sizes of dendrimers, that the sensitivity depend not only in the affinity between bioreceptors and their target molecules, but also in the physico-chemical properties of the biosensor.
Author: Man Bock Gu
Publisher: Springer
ISBN: 3642541437
Category : Technology & Engineering
Languages : en
Pages : 338
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Book Description
Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.
Author: Mahendra Rai
Publisher: Academic Press
ISBN: 0128179996
Category : Medical
Languages : en
Pages : 546
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Book Description
Nanomycotoxicology: Treating Mycotoxins in Nanoway discusses the role of nanotechnology in the detection, toxicity and management of different types of mycotoxins. Sections cover the topic of nanomycotoxicology, the application of nanotechnology for quicker, more cost-effective and precise diagnostic procedures of mycotoxins and toxicogenic fungi, and the application of nanotechnology for the management of mycotoxigenic fungi. New topics, such as the application of nanotechnology in disease management, disease forecasting, and disease resistance, mycotoxin detection, and nanodiagnostic and molecular techniques are also presented. With chapter contributions from an international group of experts, this book presents an interdisciplinary reference for scientists and researchers working in mycotoxicology, nanotechnology, mycology, plant science, and food safety. In addition, it will be a useful tool for industrial scientists investigating technologies to update their nanotoxicology and nanosafety knowledge. - Discusses the role of nanotechnology in mycotoxicology - Explores the application of nanomaterials for detection of mycotoxins - Covers the role of nanotechnology in the management of mycotoxins and mycotoxigenic fungi
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 8
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Book Description
The objective of this work is to develop a novel sensing platform for the direct and rapid detection of biological agents. To achieve this goal, we are building on our portable DNA Biological Detection System work by incorporating a new class of molecular recognition elements, known as aptamers. In this paper, the nucleic acid-based sensor work conducted in our laboratory is discussed including initial work on the design and optimization of aptamer biorecognition sensor elements targeting a relevant biological agent surrogate (i.e., Bacillus globigii) and the development of a portable nucleic acid detection system.
Author: Alan Ka Ho Cheng
Publisher:
ISBN:
Category : Biochemical markers
Languages : en
Pages : 0
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Book Description
The technique of "systematic evolution of ligands by exponential enrichment" has transformed the premise of biorecognition to encompass nucleic acids in addition to antibodies.These synthetic counterparts - aptamers - are DNA or RNA strands that have been selected through iterative rounds of in vitro selection to bind with affinity to analytes (from small molecules to proteins) of choice. In this thesis, the voltammetric aptamer-based detection of lysozyme was first explored based on charge density changes on aptamer-modified electrodes upon analyte binding. Different optical and electrochemical methods were then tested for the detection of ubiquitous glycanhydrolaselysozyme was first explored based on charge density changes on aptamer-modified electrodes upon analyte binding. Different detection methodologies (optical and electrochemical methods) were then tested for the detection of MUC1(mucin 1),a glycoprotein overexpressed in elevated concentrations in the serum of patients with epithelial adenocarcinomas; this augments the importance for MUC1 detection as an alternative for ealry cancer diagnostics. Optical biosensors developed for the MUC1 detection was through a DNA hybridization scheme with quantum-dot based flouorescence readout. Electrochemical methods were based on either direct attachment of a redox center, methylene blue(MB), to the aptamer strands,or adding free MB to the electrolyte solution(intercalative binding). In both cases, quantitative detection of MUC1 was possible, although direct labeling showed a better sensitivity. To further improve the sensitivity, an intricate biochemical assay was explored featuring the fusion of anti-thrombin and anti-MUC1 aptamers, in the hope of generating a pico-Molar assay for MUC1 via the MUC1- dependent allosteric upregulation of the proteolytic activity of human a-thrombin.
Author: Dimitrios P. Nikolelis
Publisher: Springer
ISBN: 3319289268
Category : Technology & Engineering
Languages : en
Pages : 514
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Book Description
This book offers comprehensive coverage of biomarker/biosensor interactions for the rapid detection of weapons of bioterrorism, as well as current research trends and future developments and applications. It will be useful to researchers in this field who are interested in new developments in the early detection of such. The authors have collected very valuable and, in some aspects indispensable experience in the area i.e. in the development and application of portable biosensors for the detection of potential hazards. Most efforts are centered on the development of immunochemical assays including flow-lateral systems and engineered antibodies and their fragments. In addition, new approaches to the detection of enzyme inhibitors, direct enzymatic and microbial detection of metabolites and nutrients are elaborated. Some realized prototypes and concept devices applicable for the further use as a basis for the cooperation programs are also discussed.There is a particular focus on electrochemical and optical detection systems,including those employing carbon nanotubes, quantum dots and metalnanoparticles. The authors are well-known scientists and most of them are editors of respected international scientific journals. Although recently developed biosensors utilize known principles, the biosensing devices described can significantly shorten the time required for successful detection and enhance efforts in more time-consuming directions, e.g. remote sensing systems and validation in real-sample analysis.The authors describe advances in all stages of biosensor development: theselection of biochemical components, their use in biosensor assembly, detection principles and improvements and applications for real sample assays.
Author: Irineo Torres-Pacheco
Publisher: BoD – Books on Demand
ISBN: 9533077115
Category : Medical
Languages : en
Pages : 380
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Book Description
This book is divided into three sections. The section called Aflatoxin Contamination discusses the importance that this subject has for a country like the case of China and mentions examples that illustrate the ubiquity of aflatoxins in various commodities The section Measurement and Analysis, describes the concept of measurement and analysis of aflatoxins from a historical prespective, the legal, and the state of the art in methodologies and techniques. Finally the section entitled Approaches for Prevention and Control of Aflatoxins on Crops and on Different Foods, describes actions to prevent and mitigate the genotoxic effect of one of the most conspicuous aflatoxins, AFB1. In turn, it points out interventions to reduce identified aflatoxin-induced illness at agricultural, dietary and strategies that can control aflatoxin. Besides the preventive management, several approaches have been employed, including physical, chemical biological treatments and solvent extraction to detoxify AF in contaminated feeds and feedstuffs.
Author: Raju Khan
Publisher: Elsevier
ISBN: 0128165227
Category : Technology & Engineering
Languages : en
Pages : 433
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Book Description
Advanced Biosensors for Health Care Applications highlights the different types of prognostic and diagnostic biomarkers associated with cancer, diabetes, Alzheimer's disease, brain and retinal diseases, cardiovascular diseases, bacterial infections, as well as various types of electrochemical biosensor techniques used for early detection of the potential biomarkers of these diseases. Many advanced nanomaterials have attracted intense interests with their unique optical and electrical properties, high stability, and good biocompatibility. Based on these properties, advanced nanoparticles have been used as biomolecular carriers, signal producers, and signal amplifiers in biosensor design. Recent studies reported that there are several diagnostic methods available, but the major issue is the sensitivity and selectivity of these approaches. This book outlines the need of novel strategies for developing new systems to retrieve health information of patients in real time. It explores the potential of nano-multidisciplinary science in the design and development of smart sensing technology using micro-nanoelectrodes, novel sensing materials, integration with MEMS, miniaturized transduction systems, novel sensing strategy, that is, FET, CMOS, System-on-a-Chip (SoC), Diagnostic-on-a-Chip (DoC), and Lab-on-a-Chip (LOC), for diagnostics and personalized health-care monitoring. It is a useful handbook for specialists in biotechnology and biochemical engineering. - Describes advanced nanomaterials for biosensor applications - Relates the properties of available nanomaterials to specific biomarkers applications - Includes diagnosis and electrochemical studies based on biosensors - Explores the potential of nano-multidisciplinary science to design and develop smart sensing technologies - Describes novel strategies for developing a new class of assay systems to retrieve the desired health information
Author: Gulab Singh Yadav
Publisher: Springer Nature
ISBN: 9811388369
Category : Science
Languages : en
Pages : 186
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Book Description
The book discusses the basics of aptamers and the advent of aptamer-based technology in recent times. The book covers the diverse applications of aptamers, such as in detection of animal and plant pathogens, disease diagnosis and therapeutics, environmental contamination detection etc. Besides these applications, the book also describes the use of these synthetic or modified DNA, as drug delivery vehicles. The different chapters describe how the binding capacity and specificity of aptamers can be exploited in various ways. The book also discusses how these attributes of aptamers can outdo the antibody technology in biomedical and diagnostic solutions. This crisp and concise book gives the readers an insight into the most recent biotechnological applications of aptamers.
Author: Vernon Somerset
Publisher: BoD – Books on Demand
ISBN: 9533074868
Category : Science
Languages : en
Pages : 370
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Book Description
This book is a collection of contributions from leading specialists on the topic of biosensors for health, environment and biosecurity. It is divided into three sections with headings of current trends and developments; materials design and developments; and detection and monitoring. In the section on current trends and developments, topics such as biosensor applications for environmental and water monitoring, agro-industry applications, and trends in the detection of nerve agents and pesticides are discussed. The section on materials design and developments deals with topics on new materials for biosensor construction, polymer-based microsystems, silicon and silicon-related surfaces for biosensor applications, including hybrid film biosensor systems. Finally, in the detection and monitoring section, the specific topics covered deal with enzyme-based biosensors for phenol detection, ultra-sensitive fluorescence sensors, the determination of biochemical oxygen demand, and sensors for pharmaceutical and environmental analysis.
