Defining The Teratoma as a Model for Multi-Lineage Human Development PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Defining The Teratoma as a Model for Multi-Lineage Human Development PDF full book. Access full book title Defining The Teratoma as a Model for Multi-Lineage Human Development by Daniella Nicole McDonald. Download full books in PDF and EPUB format.
Author: Daniella Nicole McDonald
Publisher:
ISBN:
Category :
Languages : en
Pages : 192
Get Book Here
Book Description
We propose that the teratoma, a recognized standard for validating pluripotency in stem cells, could be a promising platform for studying human developmental processes. Performing single cell RNA-seq of 179,632 cells across 23 teratomas from 4 cell lines, we found teratomas reproducibly contain approximately 20 cell types across all 3 germ layers, the inter-teratoma cell type heterogeneity was comparable to organoid systems, and that the teratoma gut and brain cell types correspond well to similar fetal cell types. Cellular barcoding confirmed that injected stem cells robustly engraft and contribute to all lineages. Using pooled CRISPR-Cas9 knockout screens, we showed that teratomas can simultaneously assay the effects of genetic perturbations across all germ layers. Additionally, we demonstrated teratomas can be enriched for specific lineages via a genetic or materials approach. We either molecularly sculpted via miRNA-regulated suicide gene expression or assayed teratomas under multiple matrix conditions. Taken together, the teratoma is a promising platform for modeling multi-lineage development, pan-tissue functional genetic screening, and tissue engineering.
Author: Daniella Nicole McDonald
Publisher:
ISBN:
Category :
Languages : en
Pages : 192
Get Book Here
Book Description
We propose that the teratoma, a recognized standard for validating pluripotency in stem cells, could be a promising platform for studying human developmental processes. Performing single cell RNA-seq of 179,632 cells across 23 teratomas from 4 cell lines, we found teratomas reproducibly contain approximately 20 cell types across all 3 germ layers, the inter-teratoma cell type heterogeneity was comparable to organoid systems, and that the teratoma gut and brain cell types correspond well to similar fetal cell types. Cellular barcoding confirmed that injected stem cells robustly engraft and contribute to all lineages. Using pooled CRISPR-Cas9 knockout screens, we showed that teratomas can simultaneously assay the effects of genetic perturbations across all germ layers. Additionally, we demonstrated teratomas can be enriched for specific lineages via a genetic or materials approach. We either molecularly sculpted via miRNA-regulated suicide gene expression or assayed teratomas under multiple matrix conditions. Taken together, the teratoma is a promising platform for modeling multi-lineage development, pan-tissue functional genetic screening, and tissue engineering.
Author:
Publisher: Academic Press
ISBN: 9780128134818
Category : Science
Languages : en
Pages : 150
Get Book Here
Book Description
Advances in Stem Cells and Their Niches addresses stem cells during development, homeostasis, and disease/injury of the respective organs, presenting new developments in the field, including new data on disease and clinical applications. Video content illustrates such areas as protocols, transplantation techniques, and work with mice. Explores not only reviews of research, but also shares methods, protocols, and transplantation techniques Contains video content to illustrate such areas as protocols, transplantation techniques, and work with mice Each volume concentrates on one organ, making this a unique publication
Author: Arlene Chiu
Publisher: Springer Science & Business Media
ISBN:
Category : Science
Languages : en
Pages : 488
Get Book Here
Book Description
A discussion of all the key issues in the use of human pluripotent stem cells for treating degenerative diseases or for replacing tissues lost from trauma. On the practical side, the topics range from the problems of deriving human embryonic stem cells and driving their differentiation along specific lineages, regulating their development into mature cells, and bringing stem cell therapy to clinical trials. Regulatory issues are addressed in discussions of the ethical debate surrounding the derivation of human embryonic stem cells and the current policies governing their use in the United States and abroad, including the rules and conditions regulating federal funding and questions of intellectual property.
Author: Ivan Damjanov
Publisher: Springer Science & Business Media
ISBN: 1461256283
Category : Medical
Languages : en
Pages : 369
Get Book Here
Book Description
Author: Ariff Bongso
Publisher: World Scientific
ISBN: 9814289388
Category : Science
Languages : en
Pages : 721
Get Book Here
Book Description
Stem cell biology has drawn tremendous interest in recent years as it promises cures for a variety of incurable diseases. This book deals with the basic and clinical aspects of stem cell research and involves work on the full spectrum of stem cells isolated today. It also covers the conversion of stem cell types into a variety of useful tissues which may be used in the future for transplantation therapy. It is thus aimed at undergraduates, postgraduates, scientists, embryologists, doctors, tissue engineers and anyone who wishes to gain some insight into stem cell biology. This book is important as it is comprehensive and covers all aspects of stem cell biology, from basic research to clinical applications. It will have 33 chapters written by renowned stem cell scientists worldwide. It will be up-to-date and all the chapters include self-explanatory figures, color photographs, graphics and tables. It will be easy to read and give the reader a complete understanding and state of the art of the exciting science and its applications.
Author: Patrick Cahan
Publisher: Humana
ISBN: 9781493992232
Category : Science
Languages : en
Pages : 0
Get Book Here
Book Description
This volume details methods and protocols to further the study of stem cells within the computational stem cell biology (CSCB) field. Chapters are divided into four sections covering the theory and practice of modeling of stem cell behavior, analyzing single cell genome-scale measurements, reconstructing gene regulatory networks, and metabolomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Stem Cell Biology: Methods and Protocols will be an invaluable guide to researchers as they explore stem cells from the perspective of computational biology.
Author: Jennifer A. Doudna
Publisher:
ISBN: 9781621821304
Category : Genetics
Languages : en
Pages : 0
Get Book Here
Book Description
CRISPR/Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for de.
Author: William Moy Stratton Russell
Publisher: Hyperion Books
ISBN:
Category : Nature
Languages : en
Pages : 256
Get Book Here
Book Description
Author: Wanda M Haschek
Publisher: Academic Press
ISBN: 0124157653
Category : Medical
Languages : en
Pages : 3055
Get Book Here
Book Description
Haschek and Rousseaux's Handbook of Toxicologic Pathology is a key reference on the integration of structure and functional changes in tissues associated with the response to pharmaceuticals, chemicals and biologics. The 3e has been expanded by a full volume, and covers aspects of safety assessment not discussed in the 2e. Completely revised with many new chapters, it remains the most authoritative reference on toxicologic pathology for scientists and researchers studying and making decisions on drugs, biologics, medical devices and other chemicals, including agrochemicals and environmental contaminants. New topics include safety assessment, the drug life cycle, risk assessment, communication and management, carcinogenicity assessment, pharmacology and pharmacokinetics, biomarkers in toxicologic pathology, quality assurance, peer review, agrochemicals, nanotechnology, food and toxicologic pathology, the environment and toxicologic pathology and more. Provides new chapters and in-depth discussion of timely topics in the area of toxicologic pathology and broadens the scope of the audience to include toxicologists and pathologists working in a variety of settings Offers high-quality and trusted content in a multi-contributed work written by leading international authorities in all areas of toxicologic pathology Features hundreds of full color images in both the print and electronic versions of the book to highlight difficult concepts with clear illustrations
Author: Matthew H. Kaufman
Publisher: CUP Archive
ISBN: 9780521254496
Category : Nature
Languages : en
Pages : 296
Get Book Here
Book Description
