Capillary Electrophoresis of DNA in Dilute Polymer Solutions PDF Download
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Author: Wade Michael Sunada
Publisher:
ISBN:
Category : Capillary electrophoresis
Languages : en
Pages : 390
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Book Description
Author: Wade Michael Sunada
Publisher:
ISBN:
Category : Capillary electrophoresis
Languages : en
Pages : 390
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Book Description
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 20
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Book Description
Because the mechanism of DNA separation in capillary electrophoresis is not well understood, selection of polymers is a {open_quotes}trial-and-error{close_quotes} procedure. We investigated dilute-solution DNA separations by capillary electrophoresis using solutions of four polymers that differ in size, shape and stiffness. Hydroxyethylcellulose of high molecular weight provides excellent separation of large DNA fragments (2027 bp - 23130 bp). Polyvinylpyrrolidone separates DNA from 72 bp to 23 kbp and star-(polyethylene oxide), like linear poly (ethylene oxide), provides separation of fragments up to 1353 bp.
Author: Margaret E. Starkweather
Publisher:
ISBN:
Category : Capillary electrophoresis
Languages : en
Pages : 224
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Author: Keith R. Mitchelson
Publisher: Springer Science & Business Media
ISBN: 1592590551
Category : Science
Languages : en
Pages : 481
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Book Description
The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.
Author: Annelise Emily Barron
Publisher:
ISBN:
Category :
Languages : en
Pages : 704
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Author: Jeffrey James Schwinefus
Publisher:
ISBN:
Category :
Languages : en
Pages : 316
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Author: Chunhung Wu
Publisher:
ISBN:
Category : Capillary electrophoresis
Languages : en
Pages : 322
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Author: Sourav Saha
Publisher:
ISBN:
Category :
Languages : en
Pages : 340
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Author: Christoph Heller
Publisher: Springer Science & Business Media
ISBN: 3322910156
Category : Technology & Engineering
Languages : en
Pages : 318
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Book Description
Die Kapillarelektrophorese (CE) ist heute in biochemischen Laboratorien noch nicht als Routinemethode zur DNA-Trennung etabliert, obwohl sie diverse Vorzüge gegenüber den derzeitigen Methoden besitzt. In diesem Band stellen verschiedene Wissenschaftler die theoretischen Aspekte der Trennung von Oligonucleinsäuren mit der CE, die instrumentellen Grundlagen und deren Grenzen und schließlich die praktische Anwendung in der Biochemie und Molekularbiologie vor. This book on capillary electrophoresis is unique in its focus on the separation of nucleic acids. The importance of electrophoretic separation in every molecular biology laboratory justifies this specialization, which is also reflected in the development of instrumentation. This book is aimed to help to implement this rather new and promising technology in the biological laboratories and to help to overcome typical problems that can occur when starting with a new technique. It should also trigger further development in the field. It covers the theoretical background as well as practical examples of usual applications. The authors are all experts in their field, having many years of experience with capillary electrophoresis and their application to nucleic acids.
Author: Richard W. Hammond
Publisher:
ISBN:
Category :
Languages : en
Pages :
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