Capillary Electrophoresis in Analytical Biotechnology PDF Download
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Author: Pier Giorgio Righetti
Publisher: CRC Press
ISBN: 9780849378256
Category : Medical
Languages : en
Pages : 570
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Book Description
This new book on capillary electrophoresis (CE) is unique in its focus on biotechnology. It is devoted to proteins, peptides, and techniques especially useful in the area of recombinant DNA products. Emphasis is also placed on glycoproteins. Because of the growing role of the glycosylation process in CE, a comprehensive chapter on the subject acts as a book within a book. Although this well-known researcher in biotechnology presents a number of chapters extensively discussing theories, important practical aspects in the routine use of capillary electrophoresis are also covered.
Author: Pier Giorgio Righetti
Publisher: CRC Press
ISBN: 9780849378256
Category : Medical
Languages : en
Pages : 570
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Book Description
This new book on capillary electrophoresis (CE) is unique in its focus on biotechnology. It is devoted to proteins, peptides, and techniques especially useful in the area of recombinant DNA products. Emphasis is also placed on glycoproteins. Because of the growing role of the glycosylation process in CE, a comprehensive chapter on the subject acts as a book within a book. Although this well-known researcher in biotechnology presents a number of chapters extensively discussing theories, important practical aspects in the routine use of capillary electrophoresis are also covered.
Author: Andras Guttman
Publisher: Newnes
ISBN: 0080931359
Category : Science
Languages : en
Pages : 391
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Book Description
Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. - Covers all theoretical and practical aspects of capillary gel electrophoresis - Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods - Teaches readers how to use the technology and select methods that are ideal for fundamental problems - Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses
Author: James P. Landers
Publisher: CRC Press
ISBN: 1420004956
Category : Medical
Languages : en
Pages : 1598
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Book Description
Now in its third edition, this bestselling work continues to offer state-of-the-art information on the development and employment of capillary electrophoresis. With special emphasis on microseparations and microfluidics, it features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Enhanced by contributions from leading international experts, the Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition remains a seminal reference for the chemistry, biology, and engineering fields.
Author: Thomas G.M. Schalkhammer
Publisher: Springer Science & Business Media
ISBN: 9783764365899
Category : Science
Languages : en
Pages : 356
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Book Description
Modern analytical biotechnology is focused on the use of a set of enabling platform technologies that provide contemporary, state-of-the-art tools for genomics, proteomics, metabolomics, drug discovery, screening, and analysis of natural product molecules. Thus, analytical biotechnology covers all areas of bioanalysis from biochips and nano-chemistry to biology and high throughput screening. Moreover, it aims to apply advanced automation and micro fabrica tion technology to the development of robotic and fluidic devices as well as integrated systems. This book focuses on enhancement technology development by promoting cross-disciplinary approaches directed toward solving key problems in biology and medicine. The scope thus brings under one umbrella many different techniques in allied areas. The purpose is to support and teach the fundamental principles and practical uses of major instrumental techniques. Major platforms are the use of immobilized molecules in biotechnology and bioanalysis, im munological techniques, immunological strip tests, fluorescence detection and confocal techniques, optical and electrochemical biosensors, biochips, micro dotting, novel transducers such as nano clusters, atomic force microscopy based techniques and analysis in complex media such as fermentation broth, plasma and serum. Techniques related to HPLC, capillary electrophoresis, gel electrophoresis, and mass spectrometry have not been included in this book but will be covered by further publications. Fundamentals in analytical biotechnology include basic and practical aspects of characterizing and analyzing DNA, proteins, and small metabolites.
Author: Mark A. Strege
Publisher: Springer Science & Business Media
ISBN: 159259798X
Category : Science
Languages : en
Pages : 340
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Book Description
Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive. Over time, CE has matured to become a superb complement to HLPC, and in many cases has also evolved as an automated and quantitative replacement for conventional slab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. Within Capillary Electrophoresis of Proteins and Peptides, we have assembled contributions from researchers who are applying state-of-the-art CE for protein and peptide analysis, including topics that we believe are of great potential both in the present and for the future. In comparison to traditional separation methods, CE represents a miniaturized analysis technique (especially in its microchip-based format) that is highly dependent upon the basic fundamentals of effective sample recovery and high sensitivity detection. With these issues in mind, Chapters 1–4 describe recently developed approaches for both capillary coatings and analyte detection via laser-induced fluorescence. Since the discipline of biotechnology has established itself as a primary platform for the application of CE to the analysis of proteins and peptides, Chapters 5–7 demonstrate a variety of examples of the specific techniques that have been applied for the development of biopharmaceuticals and their commercialization. The methods covered here include also the analysis of oligosaccharides from glycoproteins.
Author: Hasan Parvez
Publisher: CRC Press
ISBN: 1466563699
Category : Science
Languages : en
Pages : 495
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Book Description
This text aims to evaluate the actual impact of high-performance capillary electrophoresis on analytical biotechnology and environmental analysis. The first part of the book presents a survey of present innovations in instrument design and different methods of pre-concentration techniques in order to obtain increased separations at higher sensitivities. The second part contains articles on applications of HPCE to protein and peptide analysis. In the third part, applications of HPCE in the investigation of drug abuse and drug interactions are presented. The last two parts of the book deal with the use of HPCE at low-UV wavelengths and negative-UV absorption. The book should be of interest to those working in HPCE research and applications.
Author: Norberto A. Guzman
Publisher: CRC Press
ISBN: 9780824790424
Category : Science
Languages : en
Pages : 896
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Book Description
Tracing the sequence of observations that has led to current understanding in the field, this reference presents the basic concepts, instrumentation and applications of capillary electrophoresis, examining its many features such as high-power resolution, high-mass sensitivity, overall sensitivity and low-sample volume requirements. This work highlights the use of capillary elctrophoresis for the identification, separation, detection and characterization of substances on the molecular counting level. Illustrating the major technical maneuvers for common operations and applications, Capillary Electrophoresis Technology outlines the theoretical concepts and mathematical expressions of capillary electrophoresis; describes advances in instrumentation hardware and detection systems. It explains the advantages and limitations of the different variants of capillary electrophoresis; and provides extra coverage of areas in which capillary electrophoresis has grown increasingly popular, including the identification and characterization of small molecules and macromolecules. Written by experts in the field, this book is aimed at analytical and clinical chemists and biochemists, chemical engineers, biologists, pharmacists, biotechnologists and students in these disciplines.
Author: P. Jolles
Publisher: Springer Science & Business Media
ISBN: 9783764358853
Category : Science
Languages : en
Pages : 256
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Book Description
A wealth of information has accumulated over the last few years on the human genome. The new insights have completely changed the focus of protein analysis. It is no longer time-consuming analysis of unknown products, but rather selective identifications of individual forms, modifications and processings, and overall analysis of global protein outputs from cells and tissues in health and disease. This book gears to the rising need of sensitive, accurate, and fast separation and identification techniques in proteomics. It discusses current methodologies of modern protein analysis, from isolation and sample preparation, over analysis and identification, to final characterization. Several evaluations concentrate on the now productive approaches of two-dimensional gel electrophoresis and mass spectrometry, but alternative methods and further perspectives are also outlined. The book includes an overlook over current databases to connect protein analysis data with all available information,...
Author: Philippe Schmitt-Kopplin
Publisher: Springer Science & Business Media
ISBN: 1588295397
Category : Science
Languages : en
Pages : 808
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Book Description
This book presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques. It includes practical details which are hard to find elsewhere. The volume is intended for beginners in the field and provides an overview of the technique and a starting point for the exploration of the defined literature on different application topics.
Author: Friedrich Lottspeich
Publisher: John Wiley & Sons
ISBN: 3527694463
Category : Science
Languages : en
Pages : 1810
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Book Description
Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology
