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Author: Moshe D. White
Publisher: John Wiley & Sons
ISBN:
Category : Medical
Languages : en
Pages : 596
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Book Description
These Proceedings comprise contributions from scientists and engineers in academia and industry on practical aspects of production and purification of recombinant proteins. International, practice-oriented and detailed, the book is divided into four parts: - Choosing a production system - Propagation of recombinant microorganisms and animal cells - Purification of recombinant proteins - Health care and veterinary recombinant products Moreover, the book is interdisciplinary. This ensures that all important aspects of the production of recombinant proteins are considered. Genetic engineers, biochemical engineers, microbiologists, virologists and biochemists, protein chemists and quality control experts will find this reference book to be an indispensable guide for their daily work.
Author: Moshe D. White
Publisher: John Wiley & Sons
ISBN:
Category : Medical
Languages : en
Pages : 596
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Book Description
These Proceedings comprise contributions from scientists and engineers in academia and industry on practical aspects of production and purification of recombinant proteins. International, practice-oriented and detailed, the book is divided into four parts: - Choosing a production system - Propagation of recombinant microorganisms and animal cells - Purification of recombinant proteins - Health care and veterinary recombinant products Moreover, the book is interdisciplinary. This ensures that all important aspects of the production of recombinant proteins are considered. Genetic engineers, biochemical engineers, microbiologists, virologists and biochemists, protein chemists and quality control experts will find this reference book to be an indispensable guide for their daily work.
Author: M. D. White
Publisher:
ISBN:
Category :
Languages : en
Pages : 567
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Book Description
Author: Rewveny White S.
Publisher:
ISBN:
Category : Micrrorganisms
Languages : en
Pages : 567
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Book Description
Author: Moshe D. White
Publisher: Vch Pub
ISBN: 9780895739674
Category : Technology & Engineering
Languages : en
Pages : 567
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Book Description
Author: Otto-Wilhelm Merten
Publisher: Springer Science & Business Media
ISBN: 9780792371373
Category : Medical
Languages : en
Pages : 434
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Book Description
The general field of fundamental and applied biotechnology becomes increasingly important for the production of biologicals for human and veterinary use, by using prokaryotic and eukaryotic microorganisms. The papers in the present book are refereed articles compiled from oral and poster presentations from the EFB Meeting on Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology, which was organized in Semmering/A from 5th to 8th October 2000. A special feature of this meeting was the comparison of different classes of host cells, mainly bacteria, yeasts, filamentous fungi, and animal cells, which made obvious that many physiological features of recombinant protein formation, like cell nutrition, stress responses, protein folding and secretion, or genetic stability, follow similar patterns in different expression systems. This comparative aspect is by far the point of most interest because such comparisons are rarely done, and if they are done, their results are most often kept secret by the companies who generated them. Audience: Presently, a comparable book does not exist because the compiling of manuscripts from all fields of biotechnology (prokaryotic as well as eukaryotic, up to animal cell biotechnology) is not done in general. This particularity makes this book very interesting for postgraduate students and professionals in the large field of biotechnology who want to get a more global view on the current state of the expression of recombinant biologicals in different host cell systems, the physiological problems associated with the use of different expression systems, potential approaches to solve such difficulties by metabolic engineering or the use of other host cells, and the cooperation between process development and strain improvement, which is crucial for the optimisation of both the production strain and the process. This book should be in every library of an institution/organization involved in biotechnology.
Author: Japanese Association for Animal Cell Technology. Meeting
Publisher: Springer Science & Business Media
ISBN:
Category : Medical
Languages : en
Pages : 456
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Book Description
I. Control of Animal Cell Growth.- Novel Specific Inhibitors for Analysis of Eukaryotic Cell Cycle Control.- II. New Cell Lines.- Transgenic Animals as a Source of Genetically-Engineered Trans-Immortalised Cell Lines.- CD8+ Suppressor T Cell Clone 13G2 Secretes a Suppressive Lymphokine, Immune Suppressive Factor-T (ISF-T).- Development, Availability and Characterization of ATCC Human and Animal Cell Lines.- III. Cell Culture System Including Serum-Free Culture.- Recent Advances in Animal Cell Biotechnology.- Critical Analysis of Process Development on in-Vitro Growth of Chick-Embryo.- Improved Method for Inoculation of a Cell Suspension into a Hollow Fiber Bioreactor.- Strategies to Increase the Efficiency of Membrane Aerated and Perfused Animal Cell Bioreactors by an Improved Medium Perfusion.- Cultivation of Hepatocytes in a New Entrapment Reactor: A Potential Bioartificial Liver.- Continuous Culture with Cell Precipitation for Recombinant Protein Production.- Elimination of Microorganisms from Cell Culture Medium Using Regenerated Cellulose Hollow Fiber (BMM).- Effects of Shear Stress on the Growth of Hybridoma Cells Cultivated in Serum-Free Medium Coupled with Ammonia Removing System.- GMP Production of Biopharmaceuticals Using High Density, Fluidized-Bed Cell Culture Technology.- Anchorage-Dependent Animal Cell Growth in Porous Microcarrier Culture.- Formation of Multicellular Aggregates of Adult Rat Hepatocytes.- Operation of an Air Lift Reactor for Production of Immunochemicals by Immobilized Hybridoma Cells.- Simulation of Growth of Hybridoma Cells Immobilized in Alginate Gel Beads Based on an Oxygen Limited Model.- High Density Suspension Culture of Insect Cells in a Stirred Bioreactor.- Media for Cultivation of Animal Cells: an Overview.- Characterization of a Human Derived Macrophage-Like Cell Line, U-M.- Mitogenic Activity from Fish Embryos and the Growth of Fish Blastocyst Cells in Culture.- Protein Factor Obtained from Rat Adipose Tissue Specifically Permits the Proliferation of 3T3-L1 and OB1771 Preadipocyte Cell Lines in a Completely Defined Serum-Free Medium.- Nutrient Optimization for the Production of Biologicals from Animal Cells Cultured at High Density.- Growth and Function of Bovine Granulosa Cells Cultured in a Serum-Free Medium.- IV. Physicochemical and Biochemical Factors for Cell Growth and Production of Biologicals.- Electrically Controlled Culture of MKN45 Cells in Serum-Free Medium.- Hybridoma Culture in the Hollow-Fiber System - the Effects of Growth Factors.- Effects of Growth Factors on Hybridoma Culture in the Perfusion System.- Studies on Physiological Aspect of In-Vitro Development of Chick-Embryo.- Pilot Scale Protein Production Using Inducible Gene Amplification.- Genetic Enhancement of Protein Productivity of Animal Cells by Oncogenes.- Immunoglobulin Production Stimulation by Various Types of Caseins.- Effect of Dilution Rate on the Metabolism and Product Formation of a Recombinant Mammalian Cell Line Growing in a Chemostat with Internal Recycle of Cells.- Immunoglobulin Production Stimulating Factors in Polysaccharides.- Production of Recombinant Protein C in a Perfusion Culture.- Optimization of Cell Culture Conditions for G-CSF (Granulocyte Colony-Stimulating Factor) Production by Genetically Engineered Namalwa KJM-1 Cells.- Variation in the Ratios and Concentrations of Nucleotide Triphosphates and UDP-Sugars during a Perfused Batch Cultivation of Hybridoma Cells.- Proteolytic Activities in Serum-Free Supernatants of Mammalian Cell Lines.- Continuous Production of Erythropoietin Using a Radial Flow Bioreactor.- V. Specific Products and Their Characterization.- Effects of Autocrine Components on Growth Inhibition of NH4+ and on Growth Kinetics of Hybridoma Cells.- Glycosylation of Erythropoietin Receptor.- Effective Purification of Monoclonal Antibodies by Fast Flow Affinity Chromatography.- VI. In Vitro Assays for Toxic, Carcinogenic, and Pharmacological Effects.- Detection of Bovi...
Author: Argelia Lorence
Publisher: Humana Press
ISBN: 9781493962211
Category : Medical
Languages : en
Pages : 649
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Book Description
Studies related to recombinant gene expression have brought new advance such as the emergence of the “omics” technologies. While Escherichia coli, Sacharomyces cerevisiae and insect cells continue to be the dominant production platforms of recombinant proteins. In Recombinant Gene Expression: Review and Protocols, Third Edition, expert researchers in the field detail many of the methods now commonly used to study recombinant gene expression. These include methods and techniques for bacteria, lower eukaryotes, fungi, plants and plant cells, and animals and animal cells. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Gene Expression: Review and Protocols, Third Edition seeks to aid scientists in the further study of this crucially important research into recombinant gene expression.
Author: Donald R. Helinski
Publisher: Springer Science & Business Media
ISBN: 1461324475
Category : Medical
Languages : en
Pages : 982
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Book Description
Author: C. Exley
Publisher: Elsevier
ISBN: 0080525504
Category : Science
Languages : en
Pages : 453
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Book Description
The subject of aluminium and Alzheimer's disease has been plagued with controversy. This controversy has served to obscure much of the scientific research in this field, and subsequently has obscured the possibility that aluminium is a contributory factor in the aetiology of Alzheimer's disease. This book brings together many of the world's leading scientists researching aluminium and life and contains their critical summaries on the known facts about aluminium toxicity in man and to offer an opinion on the implications of this knowledge on a link between aluminium and Alzheimer's disease. The subject areas of the chapters were chosen to reflect the myriad of ways that aluminium is known to impact upon mammalian physiology and function and range from clinical studies, through animal models of disease to the detailed biochemistry of aluminium toxicity. Chapters are also included on epidemiology and other factors involved in the aetiology of Alzheimer's.This is the first time that this subject has been treated in such a comprehensive manner. The research detailed in each chapter, includes the latest research in the field, it has been critically appraised and this appraisal has been used by each author to present an informed opinion of its relevance to aluminium and Alzheimer's disease. The chapters are much more than reviews; they are a statement of the state of the art and of what the future may hold for research in this field. As a whole they show the high quality of research that has been carried out in our efforts to understand the toxicity of aluminium in man and that we are far away from discounting the possibility that aluminium is a contributory factor in the aetiology of Alzheimer's disease.
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Book Description
