Biodegradation of Toluene and Methyl Tert-butyl Ether (MTBE) by Pure Bacterial Cultures PDF Download
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Author: Jessica R. Hanson
Publisher:
ISBN:
Category : Butyl methyl ether
Languages : en
Pages : 176
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Book Description
Author: Jessica R. Hanson
Publisher:
ISBN:
Category : Butyl methyl ether
Languages : en
Pages : 176
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Book Description
Author: Amy Pruden
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
This study investigates the potential for biodegradation of methyl tert-butyl ether (MTBE), a prevalent gasoline additive and groundwater contaminant, under various substrate and oxidation-reduction (redox) conditions. Porous pot reactors designed for biomass retention were operated aerobically under the following conditions: MTBE only, MTBE and ethanol, MTBE and di-isopropyl ether, MTBE and diethyl ether, and MTBE with benzene, toluene, ethylbenzene, and p-xylene (BTEX). In all reactors, optimal performance was achieved when operated without biomass wasting. Mineralization of MTBE and the alternative substrates was observed in all reactors, with an average stable effluent concentration of all compounds being at or below 1 ppb (0.001 mg/l). Anaerobically, MTBE degradation was investigated in porous pot reactors and in batch systems. After a 180 day acclimation period, conversion of MTBE to its intermediate, tert-butyl alcohol (TBA), was observed in both batch and continuous-flow iron-reducing systems, and one instance of degradation was observed in the denitrifying porous pot reactor. No degradation of MTBE under methanogenic or sulfate-reducing conditions was observed after over 1 year of incubation. A molecular tool, denaturing gradient gel electrophoresis (DGGE) was used to monitor and profile the bacterial community structure of all reactors. Members of the Flexibacter-Bacterioides-Cytophaga (F-B-C) group of bacteria were most represented among all aerobic reactors, while delta-Proteobacteria were found to be prevalent in the iron-reducing system. Beta-Proteobacteria with high similarity to the known MTBE degrader, PM1, were detected by DGGE late in operation of the MTBE only and the MTBE and BTEX reactors. Two pure MTBE-degrading cultures with high similarity to PM1 were also isolated from the MTBE only reactor. Studies in pure culture also indicated no significant effect of alternative substrate (BTEX) on MTBE degradation. Results indicate that aerobic degradation of MTBE is reliable under various substrate conditions, and that a porous pot reactor designed for biomass retention is highly effective for attaining low effluent concentrations of MTBE and its intermediates.
Author: Jeffrey Hodges Scarano
Publisher:
ISBN:
Category :
Languages : en
Pages : 120
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Book Description
Author: Asunción Medrano Gener
Publisher:
ISBN:
Category :
Languages : en
Pages : 63
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Book Description
Author: Wenhua Ye
Publisher:
ISBN:
Category : Volatile organic compounds
Languages : en
Pages : 190
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Book Description
Gasoline additive Methyl Tertiary-Butyl Ether (MTBE) contaminated ground waters have been a problem nation wide with spills and leaky underground storage tanks. Biodegradation has been implemented under certain favorable environmental conditions. The biodegradation rates have been affected by treatments of nutrients, magnesium peroxide (oxygen), ethanol, temperature, pH and MTBE concentrations. This study was conducted to find the indigenous microorganisms which can degrade MTBE and assess how environmental factors would affect the biodegradation rates. This study compared MTBE biodegradation of the indigenous soil microorganisms with that of pure bacteria Methylobacterium mesophilicum, a well known MTBE degrade. In the pure culture Methylobacterium mesophilicum study, magnesium peroxide was found to enhance the MTBE biodegradation rate. However, ethanol was found to inhibit the biodegradation of MTBE, perhaps because it was degraded more easily and its metabolism decreased oxygen availability. In the soil microorganisms study, both magnesium peroxide and ethanol were found to enhance the MTBE biodegradation rates significantly. Co-metabolism between MTBE and ethanol may be possible in soil microorganisms.
Author: Robert J. Steffan
Publisher: Springer
ISBN: 9783319504322
Category : Science
Languages : en
Pages : 0
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Book Description
In this book international experts discuss the state-of-the-art in the biological degradation of hydrocarbons to meet remedial or disposal goals. The work focuses on practical applications, often on globally important scales including the remediation of some of the world’s largest crude oil spills. Other related chapters discuss important implications of microbial transformation of hydrocarbons, including treatment of high fat processing wastes, impacts of microbial biodegradation activity on industrial processes, and the implications of microbial oil degradation in relation to modern oil extraction processes like hydraulic fracturing of shales and extraction of oil sands.
Author: Maik A Jochmann
Publisher: Royal Society of Chemistry
ISBN: 1782626107
Category : Science
Languages : en
Pages : 306
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Book Description
The use of Compound-specific Stable Isotope Analysis (CSIA) is increasing in many areas of science and technology for source allocation, authentication, and characterization of transformation reactions. Until now, there have been no textbooks available for students with an analytical chemical background or basic introductory books emphasising the instrumentation and theory. This book is the first to focus solely on stable isotope analysis of individual compounds in sometimes complex mixtures. It acts as both a lecture companion for students and a consultant for advanced scientists in fields including forensic and environmental science. The book starts with a brief history of the field before going on to explain stable isotopes from scratch. The different ways to express isotope abundances are introduced together with isotope effects and isotopic fractionation. A detailed account of the required technical equipment and general procedures for CSIA is provided. This includes sections on derivatization and the use of microextraction techniques in GC-IRMS. The very important topic of referencing and calibration in CSIA is clearly described. This differs from approaches used in quantitative analysis and is often difficult for the newcomer to comprehend. Examples of successful applications of CSIA in food authenticity, forensics, archaeology, doping control, environmental science, and extraterrestrial materials are included. Applications in isotope data treatment and presentation are also discussed and emphasis is placed on the general conclusions that can be drawn from the uses of CSIA. Further instrumental developments in the field are highlighted and selected experiments are introduced that may act as a basis for a short practical course at graduate level.
Author: Eric Galimov
Publisher: Elsevier
ISBN: 0323157858
Category : Science
Languages : en
Pages : 282
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Book Description
The Biological Fractionation of Isotopes focuses on the biological fractionation of isotopes and presents calculations of the thermodynamic isotopic beta factor for polyatomic carbon compounds. This book provides experimental and theoretical evidence of the phenomenon of thermodynamically ordered distribution of isotopes in biological systems. This book consists of 12 chapters and opens with an overview of the causes of fractionation of isotopes, along with concepts such as isotopic composition and isotopic effects. The discussion then turns to the isotopic composition of the carbon of organisms; a method of calculating the thermodynamic isotopic factors of polyatomic compounds; and results of experimental investigations of intermolecular and intramolecular isotopic effects. A theoretical model of biological fractionation of isotopes is also described. The following chapters explore the regularities of the biological distribution of isotopes and present the results of some biochemical experiments, including the enzymatic decarboxylation of pyruvate and microbiological oxidation of ethanol to acetic acid. In addition, the geologic aspects of thermodynamically ordered isotopic distributions in biological products are analyzed. This monograph will be of interest to biologists, geochemists, analytical chemists, and geologists.
Author: Commission on Geosciences, Environment, and Resources
Publisher: National Academies Press
ISBN: 0309069327
Category : Science
Languages : en
Pages : 289
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Book Description
In the past decade, officials responsible for clean-up of contaminated groundwater have increasingly turned to natural attenuation-essentially allowing naturally occurring processes to reduce the toxic potential of contaminants-versus engineered solutions. This saves both money and headaches. To the people in surrounding communities, though, it can appear that clean-up officials are simply walking away from contaminated sites. When is natural attenuation the appropriate approach to a clean-up? This book presents the consensus of a diverse committee, informed by the views of researchers, regulators, and community activists. The committee reviews the likely effectiveness of natural attenuation with different classes of contaminants-and describes how to evaluate the "footprints" of natural attenuation at a site to determine whether natural processes will provide adequate clean-up. Included are recommendations for regulatory change. The committee emphasizes the importance of the public's belief and attitudes toward remediation and provides guidance on involving community stakeholders throughout the clean-up process. The book explores how contamination occurs, explaining concepts and terms, and includes case studies from the Hanford nuclear site, military bases, as well as other sites. It provides historical background and important data on clean-up processes and goes on to offer critical reviews of 14 published protocols for evaluating natural attenuation.
Author: Alexandros I. Stefanakis
Publisher: John Wiley & Sons
ISBN: 1119268346
Category : Technology & Engineering
Languages : en
Pages : 612
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Book Description
A groundbreaking book on the application of the economic and environmentally effective treatment of industrial wastewater Constructed Wetlands for Industrial Wastewater Treatment contains a review of the state-of-the-art applications of constructed wetland technology for industrial wastewater treatment. This green technology offers many economic, environmental, and societal advantages. The text examines the many unique uses and the effectiveness of constructed wetlands for the treatment of complex and heavily polluted wastewater from various industrial sources. The editor — a noted expert in the field — and the international author team (93 authors from 22 countries) present vivid examples of the current state of constructed wetlands in the industrial sector. The text is filled with international case studies and research outcomes and covers a wide range of applications of these sustainable systems including facilities such as the oil and gas industry, agro-industries, paper mills, pharmaceutical industry, textile industry, winery, brewery, sludge treatment and much more. The book reviews the many system setups, examines the different removal and/or transformational processes of the various pollutants and explores the overall effectiveness of this burgeoning technology. This important resource: Offers the first, groundbreaking text on constructed wetlands use for industrial wastewater treatment Provides a single reference with summarized information and the state-of-the-art knowledge of the use of Constructed Wetlands in the industrial sector through case studies, research outcomes and review chapters Covers a range of industrial applications such as hydrocarbons/oil and gas industry, food and beverage, wood and leather processing, agro-industries, pharmaceuticals and many others Includes best practices drawn by a collection of international case studies Presents the latest technological developments in the industry Written for civil and environmental engineers, sustainable wastewater/water managers in industry and government, Constructed Wetlands for Industrial Wastewater Treatment is the first book to offer a comprehensive review of the set-up and effectiveness of constructed wetlands for a wide range of industrial applications to highlight the diverse economic and environmental benefits this technology brings to the industry.
