Autoradiography and Immunocytochemistry PDF Download
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Author: M.A. Williams
Publisher: Elsevier
ISBN: 1483293874
Category : Science
Languages : en
Pages : 238
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Book Description
Autoradiography and Immunocytochemistry sets out in detail the preparation of autoradiographs and methods of preparing and use of immunological staining reagents. This book begins with an introduction to the demand for autoradiography and immunocytochemistry and their most advantageous use, followed by a discussion on the immunocytochemistry at the electron microscopical (EM) level in relation to the preparation of labeled antibodies. Other topics include the general remarks on “staining with antibodies; applying Fer-Ab conjugates to ultrathin sections; and controls in immunoferritin staining. The multistep antibody staining methods with unmodified proteins; control observations in staining experiments using lectins; and pattern analysis in EM immunocytochemistry are also covered. This text concludes with a description of the preparation of electron microscope autoradiographs, including the radioisotopes in EM autoradiography, preparation of radioactively-labeled tissues and cells, and methods of applying nuclear emulsions. This publication serves as a detailed laboratory guide for researchers and workers to successfully conduct electron microscope techniques.
Author: M.A. Williams
Publisher: Elsevier
ISBN: 1483293874
Category : Science
Languages : en
Pages : 238
Get Book Here
Book Description
Autoradiography and Immunocytochemistry sets out in detail the preparation of autoradiographs and methods of preparing and use of immunological staining reagents. This book begins with an introduction to the demand for autoradiography and immunocytochemistry and their most advantageous use, followed by a discussion on the immunocytochemistry at the electron microscopical (EM) level in relation to the preparation of labeled antibodies. Other topics include the general remarks on “staining with antibodies; applying Fer-Ab conjugates to ultrathin sections; and controls in immunoferritin staining. The multistep antibody staining methods with unmodified proteins; control observations in staining experiments using lectins; and pattern analysis in EM immunocytochemistry are also covered. This text concludes with a description of the preparation of electron microscope autoradiographs, including the radioisotopes in EM autoradiography, preparation of radioactively-labeled tissues and cells, and methods of applying nuclear emulsions. This publication serves as a detailed laboratory guide for researchers and workers to successfully conduct electron microscope techniques.
Author: John R.J. Baker
Publisher: Taylor & Francis
ISBN: 1000942368
Category : Medical
Languages : en
Pages : 117
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Book Description
This book describes levels of autoradiography, which is the localization within a solid specimen of a radiolabel, to help the beginner or biologist choose the correct approach for particular application. It attempts to lay emphasis on certain aspects of the appraisal of autoradiographs.
Author: Audry M. Glauert
Publisher:
ISBN:
Category : Electron microscopy
Languages : en
Pages : 248
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Book Description
Author: Lars-Inge Larsson
Publisher: CRC Press
ISBN: 1000141829
Category : Medical
Languages : en
Pages : 292
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Book Description
A complete and balanced overview of all aspects of immunocytochemistry is presented providing a clear understanding of their impact on experiment. All available techniques and many diagnostic and research applications are included, as well as practical step-by-step instructions for carrying out recommended methods. Intended for the novice as well as the experienced researchers.
Author: Laszlo Zaborszky
Publisher: Springer Science & Business Media
ISBN: 1475720556
Category : Medical
Languages : en
Pages : 429
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Book Description
This new edition presents readers with the latest information on neuroscience. This book explores the advances in molecular techniques, genomics and proteomics and the progress in fluorescence.
Author: Ennio Pannese
Publisher: Thieme
ISBN: 9780865774568
Category : Medical
Languages : en
Pages : 284
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Book Description
In the vast field of neuroscience, the introduction over the last 30 years of new investigative techniques (such as transmission and scanning electron microscopy, freeze-fracturing technique, cell organelle isolation by differential centrifugation, autoradiography, tracing techniques and immunocytochemistry) has greatly expanded our knowledge of neurocytology. This new information, however, is generally dispersed in the specialist journals or collected in reviews on specific topics. As a result, those whose interests lie in neurocytology have difficulty not only in finding data relative to their particular research, but also and above all, in gaining an overall and systematic vision of their discipline. It was this situation which prompted Ennio Pannese to systematize the major acquisitions on the minute structure of nerve and neuroglial cells and their interrelationships, correlate them with the classical concepts of light microscopy and integrate them, where possible, with elements of biochemistry and cell physiology.
Author: William V. Dashek
Publisher: Springer Science & Business Media
ISBN: 1592592325
Category : Science
Languages : en
Pages : 297
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Book Description
Hands-on experimentalists describe the cutting-edge microscopical methods needed for the effective study of plant cell biology today. These powerful techniques, all described in great detail to ensure successful experimental results, range from light microscope cytochemistry, autoradiography, and immunocytochemistry, to recent developments in fluorescence, confocal, and dark-field microscopies. Important advances in both conventional and scanning electron microscopies are also fully developed, together with such state-of-the-art ancillary techniques as high-resolution autoradiography, immunoelectron microscopy, X-ray microanalysis, and electron systems imaging. Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.
Author: Helga Kolb
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: 0 J.M. Polak and S. Van Noorden
Publisher: Taylor & Francis
ISBN: 1000947947
Category : Science
Languages : en
Pages : 170
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Book Description
This book provides a practically directed basis for carrying out immunocytochemical methods with enough theoretical information to allow a newcomer to understand the whys and wherefores of the technique. It explains tissue preparation, cytological preparations and new methods of multiple staining.
Author: Clarence H. Suelter
Publisher: John Wiley & Sons
ISBN: 0470110996
Category : Science
Languages : en
Pages : 344
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Book Description
Concerned with application of special instrumental methods to problems in biology. Describes the use of x-ray crystallography in biochemistry. Reviews the application of both transmission microscopy and scanning probe microscopy to biological problems. Discusses well-developed techniques used primarily in clinical laboratories.
