Atlas of Living Cell Cultures PDF Download
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Author: Toni Lindl
Publisher: John Wiley & Sons
ISBN: 3527669930
Category : Science
Languages : en
Pages : 521
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Book Description
The first atlas in many years giving researchers a good visual reference of the status of their cell lines. Given the increasing importance of well defined cellular models in particular in biomedical research this is a sorely needed resource for everyone performing cell culture.
Author: Toni Lindl
Publisher: John Wiley & Sons
ISBN: 3527669930
Category : Science
Languages : en
Pages : 521
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Book Description
The first atlas in many years giving researchers a good visual reference of the status of their cell lines. Given the increasing importance of well defined cellular models in particular in biomedical research this is a sorely needed resource for everyone performing cell culture.
Author: Toni Lindl
Publisher: John Wiley & Sons
ISBN: 3527669949
Category : Science
Languages : en
Pages : 470
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Book Description
The first atlas in many years giving researchers a good visual reference of the status of their cell lines. Given the increasing importance of well defined cellular models in particular in biomedical research this is a sorely needed resource for everyone performing cell culture.
Author: Hans-Peter Meyer
Publisher: John Wiley & Sons
ISBN: 3527683356
Category : Science
Languages : en
Pages : 642
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Book Description
The submersed cultivation of organisms in sterile containments or fermenters has become the standard manufacturing procedure, and will remain the gold standard for some time to come. This book thus addresses submersed cell culture and fermentation and its importance for the manufacturing industry. It goes beyond expression systems and integrally investigates all those factors relevant for manufacturing using suspension cultures. In so doing, the contributions cover all industrial cultivation methods in a comprehensive and comparative manner, with most of the authors coming from the industry itself. Depending on the maturity of the technology, the chapters address in turn the expression system, basic process design, key factors affecting process economics, plant and bioreactor design, and regulatory aspects.
Author: R. Ian Freshney
Publisher: John Wiley & Sons
ISBN: 1119513030
Category : Science
Languages : en
Pages : 832
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Book Description
FRESHNEY’S CULTURE OF ANIMAL CELLS THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES Freshney’s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more. The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text: Provides comprehensive single-volume coverage of basic skills and protocols, specialized techniques and applications, and new and emerging developments in the field Covers every essential area of animal cell culture, including lab design, disaster and contingency planning, safety, bioethics, media preparation, primary culture, mycoplasma and authentication testing, cell line characterization and cryopreservation, training, and troubleshooting Features a wealth of new content including protocols for gene delivery, iPSC generation and culture, and tumor spheroid formation Includes an updated and expanded companion website containing figures, artwork, and supplementary protocols to download and print The eighth edition of Freshney’s Culture of Animal Cells is an indispensable volume for anyone involved in the field, including undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic research scientists, and managers, technicians, and trainees working in cell biology, molecular biology, and genetics laboratories.
Author: R. Ian Freshney
Publisher: John Wiley & Sons
ISBN: 1118873645
Category : Science
Languages : en
Pages : 1615
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Book Description
Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.
Author: Boyce Rensberger
Publisher: Oxford University Press, USA
ISBN:
Category : Science
Languages : en
Pages : 314
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Book Description
In Amazing Life, Boyce Rensberger takes readers to the frontlines of cell research with some of the brightest investigators in molecular, cellular, and developmental biology. The hottest topics in biomedical research are covered.
Author: Jennie P. Mather
Publisher: Springer Science & Business Media
ISBN: 0585275718
Category : Science
Languages : en
Pages : 247
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Book Description
It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.
Author: Tamás Röszer
Publisher: Springer Nature
ISBN: 3030504808
Category : Medical
Languages : en
Pages : 224
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Book Description
Macrophages are core components of the innate immune system. Once activated, they may have either pro- or anti-inflammatory effects that include pathogen killing, safe disposal of apoptotic cells or tissue renewal. The activation state of macrophages is conceptualized by the so-called M1/M2 model of polarization. M2 macrophages are not simply antagonists of M1 macrophages; rather, they represent a network of tissue resident macrophages with roles in tissue development and organ homeostasis. M2 macrophages govern functions at the interfaces of immunity, tissue development and turnover, metabolism, and endocrine signaling. Dysfunction in M2 macrophages can ruin the healthy interplay between the immune system and metabolic processes, and lead to diseases such as insulin resistance, metabolic syndrome, and type 1 and 2 diabetes mellitus. Furthermore, M2 macrophages are essential for healthy tissue development and immunological self-tolerance. Worryingly, these functions of M2 macrophages can also be disrupted, resulting in tumor growth and autoimmunity. This book comprehensively discusses the biology of M2 macrophages, summarizes the current state of knowledge, and highlights key questions that remain unanswered.
Author: R. Ian Freshney
Publisher: Wiley-Liss
ISBN: 9780471589662
Category : Science
Languages : en
Pages : 510
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Book Description
This masterful third edition of Freshney's Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the novice and the experiences researcher to apply the basic and more sophisticated techniques of tissue culture. New Topics covered include: the use of molecular techniques in cell culture, such as DNA fingerprinting, fluorescence in situ hybridization, and chromosome painting cell interactions in cell culture new methods for separating cells new or refined methods for accessing cytotoxicity, viability, and mutagenicity experimental details for culture of specialized cells types not covered in previous editions new or refined techniques for visualizing clues, including time-lapse photography and confocal microscopy The revised and expanded third edition offers the following features: over 350 new reference to the primary literature an international list of cell banks an international listing of reagants and commercial supplies a subject index a glossary Also available: 0471169021 Culture of Animal Cells: A Multimedia Guide CD-ROM $150 est. From the reviews: "I strongly recommend this volume for any laboratory wishing to culture mammalian cells" - Biotechnology "It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" - Cell Biology International Reports
Author: Bernadette F. Rodak
Publisher: Saunders
ISBN: 9780323322492
Category : Blood
Languages : en
Pages : 0
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Book Description
Ensure you are accurately identifying cells at the microscope with Clinical Hematology Atlas, 5th Edition. An excellent companion to Rodak's Hematology: Clinical Principles & Applications, this award-winning atlas offers complete coverage of the basics of hematologic morphology, including examination of the peripheral blood smear, basic maturation of the blood cell lines, and information on a variety of clinical disorders. Nearly 500 photomicrographs, schematic diagrams, and electron micrographs vividly illustrate hematology from normal cell maturation to the development of various pathologies so you can be sure you're making accurate conclusions in the lab. Schematic diagrams, photomicrographs, and electron micrographs are found in every chapter to visually enhance understanding of hematologic cellular morphology. Smaller trim size, concise text, and spiral binding make it easy to reference the atlas in the laboratory. Chapter on normal newborn peripheral blood morphology covers the normal cells found in neonatal blood. Chapter on body fluids illustrates the other fluids found in the body besides blood, using images from cytocentrifuged specimens. The most common cytochemical stains, along with a summary chart for interpretation, are featured in the leukemia chapters to help classify both malignant and benign leukoproliferative disorders. Chapter featuring morphologic changes after myeloid hematopoietic growth factors is included in the text. Morphologic abnormalities are covered in the chapters on erythrocytes and leukocytes, along with a description of each cell, in a schematic fashion. User resources on the Evolve companion site feature review questions and summary tables to further enhance their learning experience. NEW! Appendix with comparison tables of commonly confused cells includes lymphocytes versus neutrophilic myelocytes and monocytes versus reactive lymphoctyes to help users see the subtle differences between them. NEW! Glossary of hematologic terms at the end of the book provides a quick reference to easily look up definitions.
