Author: Christopher Alan Bauser
Publisher:
ISBN:
Category :
Languages : en
Pages : 310
Book Description
Analysis of Escherichia Coli RNA Polymerase Transcription Initiation Complexes Using Photochemical Crosslinking
Author: Christopher Alan Bauser
Publisher:
ISBN:
Category :
Languages : en
Pages : 310
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 310
Book Description
Investigation of Escherichia coli RNA polymerase-promoter complexes formed during transcription initiation
Author: Xiao-Yong Li
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
Book Description
Structure and Function Relationships in Escherichia Coli RNA Polymerase
Author: Julie Yeager-Zink
Publisher:
ISBN:
Category :
Languages : en
Pages : 214
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 214
Book Description
Atomic Force Microscopy of Transcription Initiation Complexes Formed by Escherichia Coli RNA Polymerase
Author: Sebastian Maurer
Publisher:
ISBN:
Category :
Languages : en
Pages : 158
Book Description
As much as protein - DNA interactions depend on the conformation of the binding protein they are also influenced by the local geometry of the DNA. Since the topology of DNA is continuously changing due to ongoing replication, transcription and associated fluctuations of topoisomerase activity, the accessibility and configuration of the recognition elements for proteins, the major grooves for example, are also subject to changes. Thus many protein-DNA interactions are affected by a change of the superhelicity of DNA, although to different extent. The work at hand focuses on the effect of DNA topology on regulation of gene transcription - a relationship studied on the example of two supercoiling sensitve E.Coli promoters: The fis (factor of inversion stimulation) promoter and the Tyrosine tRNA promoter. By utilizing Atomic Force Microscopy (AFM) this study demonstrates both: a complex of two RNAP molecules forming a dimer on binding to the divergent promoter module consisting of fisP1 and fisPdiv sites, as well as the structures of FIS and RNAP bound to the tyrT promoter either separately or together - thereby visualizing a รณ70-RNAP containing transcription initiation complex for the first time. The models for the transcriptional activation of the fis and the tyrT promoters presented in this work were proposed under consideration of the nucleoprotein complex structures visualized by AFM and supported by further biochemical data. Despite the differences in sequence organisation of the fis and tyrT promoters, both models share a common feature: A transcription activation mechanism which acts through the optimization of the local superhelical density.
Publisher:
ISBN:
Category :
Languages : en
Pages : 158
Book Description
As much as protein - DNA interactions depend on the conformation of the binding protein they are also influenced by the local geometry of the DNA. Since the topology of DNA is continuously changing due to ongoing replication, transcription and associated fluctuations of topoisomerase activity, the accessibility and configuration of the recognition elements for proteins, the major grooves for example, are also subject to changes. Thus many protein-DNA interactions are affected by a change of the superhelicity of DNA, although to different extent. The work at hand focuses on the effect of DNA topology on regulation of gene transcription - a relationship studied on the example of two supercoiling sensitve E.Coli promoters: The fis (factor of inversion stimulation) promoter and the Tyrosine tRNA promoter. By utilizing Atomic Force Microscopy (AFM) this study demonstrates both: a complex of two RNAP molecules forming a dimer on binding to the divergent promoter module consisting of fisP1 and fisPdiv sites, as well as the structures of FIS and RNAP bound to the tyrT promoter either separately or together - thereby visualizing a รณ70-RNAP containing transcription initiation complex for the first time. The models for the transcriptional activation of the fis and the tyrT promoters presented in this work were proposed under consideration of the nucleoprotein complex structures visualized by AFM and supported by further biochemical data. Despite the differences in sequence organisation of the fis and tyrT promoters, both models share a common feature: A transcription activation mechanism which acts through the optimization of the local superhelical density.
Structure/function Analysis of the Two Largest Subunits of Escherichia Coli RNA Polymerase
Author: Laura Magdolna Heisler
Publisher:
ISBN:
Category :
Languages : en
Pages : 496
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 496
Book Description
Quantitative Photoaffinity Labeling of Escherichia Coli RNA Polymerase Transcription Complexes
Author: Thomas Michael Stackhouse
Publisher:
ISBN:
Category :
Languages : en
Pages : 330
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 330
Book Description
The Interaction of E. Coli RNA Polymerase with Lambda Phage PR Promoter
Author: Won-Chul Suh
Publisher:
ISBN:
Category :
Languages : en
Pages : 430
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 430
Book Description
Studies of DNA-RNA Interactions in E. Coli RNA Polymerase Transcription Complexes
Author: Ji Lu
Publisher:
ISBN:
Category :
Languages : en
Pages : 104
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 104
Book Description
Footprinting Analysis of E. Coli RNA Polymerase Open Complexes Using KMnO4 and DNase I
Author: Sarah C. Pillard
Publisher:
ISBN:
Category : Escherichia coli
Languages : en
Pages : 152
Book Description
Publisher:
ISBN:
Category : Escherichia coli
Languages : en
Pages : 152
Book Description
Characterization by DNA Footprinting of Intermediates Involved in Transcription Initiation by Escherichia Coli E Sigma70 RNA Polymerase at the Lambda PR Promoter
Author: Marni Raffaelle
Publisher:
ISBN:
Category :
Languages : en
Pages : 242
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 242
Book Description