An Amino Acid Mixture Enhances Insulin-stimulated Glucose Uptake in Isolated Epitrochlearis Muscle PDF Download
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Author: Maximilian Kleinert
Publisher:
ISBN:
Category :
Languages : en
Pages : 132
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Book Description
Amino acids are important modulators of skeletal muscle metabolism, but their impact on glucose uptake by skeletal muscle remains unclear. To address the effect of an amino acid (AA) mixture consisting predominately of isoleucine on glucose uptake we first conducted a dose-response experiment, investigating how different concentrations of the AA mixture affect glucose uptake by isolated rat epitrochlearis muscle. In a subsequent experiment we examined how the AA mixture affects insulin-stimulated glucose uptake by isolated rat epitrochlearis muscle. It was found that the AA mixture with as little as 0.5 mM Ile increases [H3]2-deoxy-D-glucose (2-DG) uptake by 76% compared to basal glucose uptake. The AA mixtures with 1, 2 or 4 mM Ile provided no significant additional effect. Next we combined the AA mixture consisting of 2 mM Ile, 0.012 mM Cys, 0.006 mM Val and 0.014 mM Leu with physiological levels (75 [mu]U/ml, sINS) and maximally-stimulating levels (2 mU/ml, mINS) of insulin. The AA mixture only, sINS and mINS significantly increased 2-DG uptake compared to basal by 63, 79 and 298%, respectively. When the AA mixture was combined with sINS and mINS 2-DG uptake was further increased significantly by 26 and 14%, respectively. Western blotting analysis revealed that compared to basal the AA mixture increased AS160 phosphorylation, while phosphorylation of Akt and mTOR did not change. Combining the AA mixture with sINS resulted in no additional phosphorylation compared to sINS alone. Interestingly, addition of the AA mixture to mINS resulted in increased phosphorylation of mTOR, Akt and AS160 compared to mINS alone. Our results suggest that certain AAs (1) increase glucose uptake in the absence of insulin and (2) augment insulin-stimulated glucose uptake in an additive manner. These effects on glucose uptake appear to be mediated via a molecular pathway that is partially independent from the canonical insulin signaling cascade.
Author: Maximilian Kleinert
Publisher:
ISBN:
Category :
Languages : en
Pages : 132
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Book Description
Amino acids are important modulators of skeletal muscle metabolism, but their impact on glucose uptake by skeletal muscle remains unclear. To address the effect of an amino acid (AA) mixture consisting predominately of isoleucine on glucose uptake we first conducted a dose-response experiment, investigating how different concentrations of the AA mixture affect glucose uptake by isolated rat epitrochlearis muscle. In a subsequent experiment we examined how the AA mixture affects insulin-stimulated glucose uptake by isolated rat epitrochlearis muscle. It was found that the AA mixture with as little as 0.5 mM Ile increases [H3]2-deoxy-D-glucose (2-DG) uptake by 76% compared to basal glucose uptake. The AA mixtures with 1, 2 or 4 mM Ile provided no significant additional effect. Next we combined the AA mixture consisting of 2 mM Ile, 0.012 mM Cys, 0.006 mM Val and 0.014 mM Leu with physiological levels (75 [mu]U/ml, sINS) and maximally-stimulating levels (2 mU/ml, mINS) of insulin. The AA mixture only, sINS and mINS significantly increased 2-DG uptake compared to basal by 63, 79 and 298%, respectively. When the AA mixture was combined with sINS and mINS 2-DG uptake was further increased significantly by 26 and 14%, respectively. Western blotting analysis revealed that compared to basal the AA mixture increased AS160 phosphorylation, while phosphorylation of Akt and mTOR did not change. Combining the AA mixture with sINS resulted in no additional phosphorylation compared to sINS alone. Interestingly, addition of the AA mixture to mINS resulted in increased phosphorylation of mTOR, Akt and AS160 compared to mINS alone. Our results suggest that certain AAs (1) increase glucose uptake in the absence of insulin and (2) augment insulin-stimulated glucose uptake in an additive manner. These effects on glucose uptake appear to be mediated via a molecular pathway that is partially independent from the canonical insulin signaling cascade.
![Activation of Skeletal Muscle Glucose Uptake by Am [i.e. An] Amino Acid Mixture and Its Impact on Glucose Tolerance and Insulin Resistance PDF](https://books.google.com/books/content/images/frontcover/pVODAQAACAAJ?fife=w80-h100)
Author: Jeffrey Richard Bernard
Publisher:
ISBN:
Category :
Languages : en
Pages : 456
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Book Description
Recent research suggests that amino acids can significantly increase skeletal muscle glucose uptake. However, the mechanism(s) have not been fully elucidated and it is also not clear if the beneficial impact amino acids have on healthy tissue translates to insulin resistant skeletal muscle. Therefore, in this series of studies, the effects of an amino acid mixture on glucose tolerance and insulin resistance were investigated. Study 1 Experiment-1 (Exp-1) demonstrated that an amino acid mixture significantly reduced the blood glucose response to an oral glucose challenge in Sprague Dawley rats. In Study 1 Exp-2, it was found that the improved glucose tolerance was due to an increase in skeletal muscle glucose uptake. The enhanced amino acid induced muscle glucose uptake was associated with improved cellular signaling. In Study 1 we could not determine the combined and/or individual effects of insulin and amino acids on glucose uptake, so in Study 2, the hindlimb of Sprague Dawley rats were perfused with glucose with or without amino acids in the presence and absence of insulin. Study 2, confirmed our previous findings that an amino acid mixture increased skeletal muscle glucose uptake compared to a carbohydrate supplement in the presence of insulin. The enhanced amino acid-stimulated glucose uptake was not due to increased phosphatidylinositol 3-kinase (PI 3-kinase) activity, although it was related to an increase in Akt substrate of 160 kDa (AS160) phosphorylation and a greater number of glucose transporters at the plasma membrane. In the final experiment, Study 3 investigated whether amino acids could improve glucose tolerance in an insulin resistant model. Study 3 Exp-1, demonstrated that an amino acid mixture significantly lowered the blood glucose response to an oral glucose challenge in obese Zucker rats. Study 3 Exp-2 showed that the improved glucose tolerance was due to enhanced amino acid induced skeletal muscle glucose uptake. Taken together, the results of this research suggests that adding an amino acid mixture to a carbohydrate supplement improves the blood glucose response to an oral glucose challenge, acutely lowers insulin resistance and this appears due to increased skeletal muscle glucose clearance and enhanced cellular signaling.
Author:
Publisher: ScholarlyEditions
ISBN: 1464990980
Category : Medical
Languages : en
Pages : 1127
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Book Description
Advances in Proinsulin Research and Application / 2012 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Proinsulin. The editors have built Advances in Proinsulin Research and Application / 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Proinsulin in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Advances in Proinsulin Research and Application / 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Author: Ronald J. Maughan
Publisher: John Wiley & Sons
ISBN: 1118692357
Category : Medical
Languages : en
Pages : 980
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Book Description
It is well understood that proper nutrition has a significant impact on sports performance. All of the essential nutrients must be supplied in the right amounts and at the right times for an athlete to achieve optimal health and performance. In addition, when devising eating strategies that will help athletes meet their goals, sports nutritionists must take account of personal preferences, social and cultural issues, and a whole range of other factors. This latest volume in the Encyclopaedia of Sports Medicine series, published by Wiley in partnership with the Medical Commission of the International Olympic Committee, Sports Nutrition covers this dynamic field in unparalleled depth and breadth, from the scientific underpinnings of nutritional science to the development of practical nutritional programs for athletes in a range of sports. Written and edited by the world’s leading authorities on nutrition in sports, this timely new reference: Provides comprehensive coverage of nutrition for both individual and team sports Presents current knowledge of macronutrients, micronutrients, and dietary supplements for the athlete, outlining both benefits and risks Offers clear guidance on the unique nutritional needs of special populations of athletes, such as vegetarian athletes, young athletes and aging athletes Includes chapters on the clinical nutritional needs of diabetic athletes and athletes with weight management issues Carries the full endorsement of the IOC Medical Commission
Author: Mario D. Cordero
Publisher: Springer
ISBN: 3319435892
Category : Medical
Languages : en
Pages : 519
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Book Description
AMPK has emerged as an important integrator of signals that control energy balance through the regulation of multiple biochemical pathways in eukaryotes. This book focuses on the implications of AMPK as a master metabolic regulator in diseases, including new methods and animal models. The contributions are written by leading experts in the field and give an extensive overview of the current knowledge of AMPK biology and the role of AMPK in health and disease.
Author: Rudy Bilous
Publisher: John Wiley & Sons
ISBN: 1118975979
Category : Medical
Languages : en
Pages : 322
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Book Description
HANDBOOK OF DIABETES The Handbook of Diabetes provides concise and efficient coverage of the diagnosis, epidemiology, and management of diabetes and its complications. Containing hundreds of attractive colour diagrams, illustrations, and clinical photographs, this popular quick-reference guide focuses on the management and measurement of diabetes mellitus with highly visual references. Now in its fifth edition, this market-leading book aligns with the most recent guidelines from the American Diabetes Association (ADA), the European Association for the Study of Diabetes (EASD), Diabetes UK, and the National Institute for Health and Care Excellence (NICE), presenting authoritative clinical coverage of diabetes in an accessible format with rich pedagogical features. Five new chapters provide detailed coverage of liver disease, diabetes education, bariatric surgery, diabetes and cancer, and the use of incretin-based therapies and SGLT2 Inhibitors in the management of Type II diabetes Updated and expanded topics include the relation between hypoglycaemia and dementia, anxiety and depression, the NICE Quality and Outcomes Framework (QOF), and the impacts of diabetes to self-care, mental health, and decision-making Provides a wealth of pedagogical features such as vignettes and case histories, important learning points, summaries of key clinical trials, and links to further readings Handbook of Diabetes, remains the essential practical companion for all health professionals involved in managing patients with diabetes, and an up-to-date reference for diabetes and endocrinology researchers, scientists, and academics.
Author: Ashok K. Srivastava
Publisher: Springer Science & Business Media
ISBN: 9780792381136
Category : Science
Languages : en
Pages : 206
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Book Description
In 1996 the 75th anniversary of the discovery of insulin was celebrated at the University of Toronto, the scene of that discovery in 1921. This volume was stimulated by the scientific program which was staged at that time and brought together much of the world's best talent to discuss and analyze the most recent developments in our understanding of pancreatic function, insulin secretion, the interaction of insulin with its target tissues, the mechanism of insulin action at the cellular level, and the defects which underlie both Type I (insulin-dependent diabetes mellitus, IDDM) and Type II (noninsulin-dependent diabetes mellitus, NIDDM) forms of the disease. We have chosen to focus the present volume on work related to insulin action.
Author: Institut national du sport, de l'expertise et de la performance (France)
Publisher:
ISBN: 9782865802296
Category :
Languages : en
Pages : 474
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Book Description
Author: Ronald J. Maughan
Publisher: John Wiley & Sons
ISBN: 0470693010
Category : Medical
Languages : en
Pages : 699
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Book Description
As sports have become more competitive over recent years researchers and trainers have been searching for new and innovative ways of improving performance. Ironically, an area as mundane as what an athlete eats can have profound effects on fitness, health and ultimately, performance in competition. Sports have also gained widespread acceptance in the therapeutic management of athletes with disorders associated with nutritional status. In addition, exercise has been one of the tools used for studying the control of metabolism, creating a wealth of scientific information that needs to be placed in the context of sports medicine and science. Nutrition in Sport provides an exhaustive review of the biochemistry and physiology of eating. The text is divided into three sections and commences with a discussion of the essential elements of diet, including sections on carbohydrates, proteins, fats, vitamins and trace elements, and drugs associated with nutrition. It also discusses athletes requiring special consideration, including vegetarians and diabetics. The second section considers the practical aspects of sports nutrition and discusses weight control (essential for sports with weight categories and athletes with eating disorders), the travelling athlete (where travel either disrupts established feeding patterns or introduces new hazards), environmental aspects of nutrition (including altitude and heat), and the role of sports nutritional products.
Author: Sujit Kumar Bhutia
Publisher: Springer Nature
ISBN: 9811569304
Category : Medical
Languages : en
Pages : 284
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Book Description
This book deals with the paradoxical role of autophagy in tumor suppression and tumor promotion in cancer cells. Autophagy plays opposing, context-dependent roles in tumors; accordingly, strategies based on inhibiting or stimulating autophagy could offer as potential cancer therapies. The book elucidates the physiological role of autophagy in modulating cancer metastasis, which is the primary cause of cancer-associated mortality. Further, it reviews its role in the differentiation, development, and activation of multiple immune cells, and its potential applications in tumor immunotherapy. In addition, it examines the effect of epigenetic modifications of autophagy-associated genes in regulating tumor growth and therapeutic response and summarizes autophagy’s role in the development of resistance to a variety of anti-cancer drugs in cancer cells. In closing, it assesses autophagy as a potential therapeutic target for cancer treatment. Given its scope, the book offers a valuable asset for all oncologists and researchers who wish to understand the potential role of autophagy in tumor biology.
