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Author: Jerzy Kulski
Publisher: BoD – Books on Demand
ISBN: 9535122401
Category : Medical
Languages : en
Pages : 466
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Book Description
Next generation sequencing (NGS) has surpassed the traditional Sanger sequencing method to become the main choice for large-scale, genome-wide sequencing studies with ultra-high-throughput production and a huge reduction in costs. The NGS technologies have had enormous impact on the studies of structural and functional genomics in all the life sciences. In this book, Next Generation Sequencing Advances, Applications and Challenges, the sixteen chapters written by experts cover various aspects of NGS including genomics, transcriptomics and methylomics, the sequencing platforms, and the bioinformatics challenges in processing and analysing huge amounts of sequencing data. Following an overview of the evolution of NGS in the brave new world of omics, the book examines the advances and challenges of NGS applications in basic and applied research on microorganisms, agricultural plants and humans. This book is of value to all who are interested in DNA sequencing and bioinformatics across all fields of the life sciences.
Author: Jerzy Kulski
Publisher: BoD – Books on Demand
ISBN: 9535122401
Category : Medical
Languages : en
Pages : 466
Get Book Here
Book Description
Next generation sequencing (NGS) has surpassed the traditional Sanger sequencing method to become the main choice for large-scale, genome-wide sequencing studies with ultra-high-throughput production and a huge reduction in costs. The NGS technologies have had enormous impact on the studies of structural and functional genomics in all the life sciences. In this book, Next Generation Sequencing Advances, Applications and Challenges, the sixteen chapters written by experts cover various aspects of NGS including genomics, transcriptomics and methylomics, the sequencing platforms, and the bioinformatics challenges in processing and analysing huge amounts of sequencing data. Following an overview of the evolution of NGS in the brave new world of omics, the book examines the advances and challenges of NGS applications in basic and applied research on microorganisms, agricultural plants and humans. This book is of value to all who are interested in DNA sequencing and bioinformatics across all fields of the life sciences.
Author: Applied Research Press
Publisher: Createspace Independent Publishing Platform
ISBN: 9781515216209
Category :
Languages : en
Pages : 42
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Book Description
Next generation sequencing (NGS) technology has revolutionized genomic and genetic research. The pace of change in this area is rapid with three major new sequencing platforms having been released in 2011: Ion Torrent's PGM, Pacific Biosciences' RS and the Illumina MiSeq. Here we compare the results obtained with those platforms to the performance of the Illumina HiSeq, the current market leader. In order to compare these platforms, and get sufficient coverage depth to allow meaningful analysis, we have sequenced a set of 4 microbial genomes with mean GC content ranging from 19.3 to 67.7%. Together, these represent a comprehensive range of genome content. Here we report our analysis of that sequence data in terms of coverage distribution, bias, GC distribution, variant detection and accuracy. All three fast turnaround sequencers evaluated here were able to generate usable sequence. However there are key differences between the quality of that data and the applications it will support. Proceeds from the sale of this book go to the support of an elderly disabled person.
Author: Ion Mandoiu
Publisher: John Wiley & Sons
ISBN: 1118169484
Category : Computers
Languages : en
Pages : 460
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Book Description
Introduces readers to core algorithmic techniques for next-generation sequencing (NGS) data analysis and discusses a wide range of computational techniques and applications This book provides an in-depth survey of some of the recent developments in NGS and discusses mathematical and computational challenges in various application areas of NGS technologies. The 18 chapters featured in this book have been authored by bioinformatics experts and represent the latest work in leading labs actively contributing to the fast-growing field of NGS. The book is divided into four parts: Part I focuses on computing and experimental infrastructure for NGS analysis, including chapters on cloud computing, modular pipelines for metabolic pathway reconstruction, pooling strategies for massive viral sequencing, and high-fidelity sequencing protocols. Part II concentrates on analysis of DNA sequencing data, covering the classic scaffolding problem, detection of genomic variants, including insertions and deletions, and analysis of DNA methylation sequencing data. Part III is devoted to analysis of RNA-seq data. This part discusses algorithms and compares software tools for transcriptome assembly along with methods for detection of alternative splicing and tools for transcriptome quantification and differential expression analysis. Part IV explores computational tools for NGS applications in microbiomics, including a discussion on error correction of NGS reads from viral populations, methods for viral quasispecies reconstruction, and a survey of state-of-the-art methods and future trends in microbiome analysis. Computational Methods for Next Generation Sequencing Data Analysis: Reviews computational techniques such as new combinatorial optimization methods, data structures, high performance computing, machine learning, and inference algorithms Discusses the mathematical and computational challenges in NGS technologies Covers NGS error correction, de novo genome transcriptome assembly, variant detection from NGS reads, and more This text is a reference for biomedical professionals interested in expanding their knowledge of computational techniques for NGS data analysis. The book is also useful for graduate and post-graduate students in bioinformatics.
Author: Mourad Elloumi
Publisher: Springer
ISBN: 3319598260
Category : Computers
Languages : en
Pages : 356
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Book Description
The 14 contributed chapters in this book survey the most recent developments in high-performance algorithms for NGS data, offering fundamental insights and technical information specifically on indexing, compression and storage; error correction; alignment; and assembly. The book will be of value to researchers, practitioners and students engaged with bioinformatics, computer science, mathematics, statistics and life sciences.
Author: Amit Chaudhary
Publisher: Springer Nature
ISBN: 9819771234
Category :
Languages : en
Pages : 440
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Book Description
Author: Sara El-Metwally
Publisher: Springer Science & Business
ISBN: 1493907158
Category : Science
Languages : en
Pages : 123
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Book Description
The introduction of Next Generation Sequencing (NGS) technologies resulted in a major transformation in the way scientists extract genetic information from biological systems, revealing limitless insight about the genome, transcriptome and epigenome of any species. However, with NGS, came its own challenges that require continuous development in the sequencing technologies and bioinformatics analysis of the resultant raw data and assembly of the full length genome and transcriptome. Such developments lead to outstanding improvements of the performance and coverage of sequencing and improved quality for the assembled sequences, nevertheless, challenges such as sequencing errors, expensive processing and memory usage for assembly and sequencer specific errors remains major challenges in the field. This book aims to provide brief overviews the NGS field with special focus on the challenges facing the NGS field, including information on different experimental platforms, assembly algorithms and software tools, assembly error correction approaches and the correlated challenges.
Author: Khalid Rehman Hakeem
Publisher: Springer
ISBN: 3319317032
Category : Science
Languages : en
Pages : 522
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Book Description
To comprehend the organizational principle of cellular functions at diff erent levels, an integrative approach with large-scale experiments, the so-called ‘omics’ data including genomics, transcriptomics, proteomics, and metabolomics, is needed. Omics aims at the collective characterization and quantifi cation of pools of biological molecules that translate into the structure, function, and dynamics of an organism or organisms. Currently, omics is an essential tool to understand the molecular systems that underlie various plant functions. Furthermore, in several plant species, the development of omicsresources has progressed to address the particular biological properties of individual species. Integration of knowledge from omics-based research is an emerging issue as researchers seek to identify significance, gain biological insights and promote translational research. From these perspectives, we intend to provide the emerging aspects of plant systems research based on omics and bioinformatics analyses together with their associated resources and technological advances. Th e present book covers a wide range of omics topics, and discusses the latest trends and application area of plant sciences. In this volume, we have highlighted the working solutions as well as open problems and future challenges in plant omics studies. We believe that this book will initiate and introduce readers to state-of-the-art developments and trends in omics-driven research.
Author: Vijai Singh
Publisher: Springer Nature
ISBN: 9819984017
Category :
Languages : en
Pages : 365
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Book Description
Author: Pankaj Kumar Arora
Publisher: Springer Nature
ISBN: 981138844X
Category : Science
Languages : en
Pages : 343
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Book Description
This book describes various aspects of modern microbiology including microbial enzymes, secondary metabolites, next-generation sequencing, microbial-based biopesticides, microbial-based cancer therapies, biodiesel, and microbial products from fermentation, biodegradation, bioremediation and wastewater treatment. Further, it explains how and why microbes play an important role in preserving the welfare of living beings and the environment. Many bacteria play a significant part in cleaning our environment by detoxifying various xenobiotic compounds, while several microbes produce secondary metabolites that are useful to human beings. The book is divided into 15 chapters that cover various aspects of microorganism-based biotechnology, including recent methodologies such as advanced molecular techniques, as well developments in classical microbiological techniques. The authors also explain how the latest and classical techniques are being used in modern-day microbial biotechnology. All chapters were written by experts from prominent universities, research laboratories, and institutes around the globe. Above all, they focus on recent advances in microbial technology that promote the welfare of living beings and the environment.
Author: Hirak Ranjan Dash
Publisher: Elsevier
ISBN: 032399380X
Category : Science
Languages : en
Pages : 614
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Book Description
Next Generation Sequencing (NGS) Technology in DNA Analysis explains and summarizes next generation sequencing (NGS) technological applications in the field of forensic DNA analysis. The book covers the transition from capillary electrophoresis (CE)-based technique to NGS platforms and the fundamentals of NGS technologies, applications, and advances. Sections provide an overview of NGS technology and forensic science, including information on processing biological samples for forensic analysis, sequence analysis, and data analysis software as well as the analysis of NGS data. The book explores the valuable applications of NGS-based forensic DNA analysis and covers the validations and interpretation guidelines of NGS workflows. With chapter contributions from an international array of experts and the inclusion of practical case studies, this book is a useful reference for academicians and researchers in genetics, biotechnology, bioinformatics, biology, and medicine as well as forensic DNA scientists and practitioners who aim to learn, use, apply, and validate NGS-based technologies. - Describes the fundamentals of NGS and its advances for forensic applications - Explains the transition from CE-based technique to NGS technology - Includes case studies related to NGS and DNA fingerprinting - Explores the future use and applications of NGS technologies
