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Author: Anne Straube
Publisher: Humana Press
ISBN: 9781493961856
Category : Science
Languages : en
Pages : 319
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Book Description
Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range of well-established and newly-emerging techniques in this vital field.
Author: Anne Straube
Publisher: Humana Press
ISBN: 9781493961856
Category : Science
Languages : en
Pages : 319
Get Book Here
Book Description
Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range of well-established and newly-emerging techniques in this vital field.
Author: Roberto de Virgilio
Publisher: Springer Science & Business Media
ISBN: 3642043291
Category : Computers
Languages : en
Pages : 554
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Book Description
Databases have been designed to store large volumes of data and to provide efficient query interfaces. Semantic Web formats are geared towards capturing domain knowledge, interlinking annotations, and offering a high-level, machine-processable view of information. However, the gigantic amount of such useful information makes efficient management of it increasingly difficult, undermining the possibility of transforming it into useful knowledge. The research presented by De Virgilio, Giunchiglia and Tanca tries to bridge the two worlds in order to leverage the efficiency and scalability of database-oriented technologies to support an ontological high-level view of data and metadata. The contributions present and analyze techniques for semantic information management, by taking advantage of the synergies between the logical basis of the Semantic Web and the logical foundations of data management. The book’s leitmotif is to propose models and methods especially tailored to represent and manage data that is appropriately structured for easier machine processing on the Web. After two introductory chapters on data management and the Semantic Web in general, the remaining contributions are grouped into five parts on Semantic Web Data Storage, Reasoning in the Semantic Web, Semantic Web Data Querying, Semantic Web Applications, and Engineering Semantic Web Systems. The handbook-like presentation makes this volume an important reference on current work and a source of inspiration for future development, targeting academic and industrial researchers as well as graduate students in Semantic Web technologies or database design.
Author: George Lipscomb
Publisher:
ISBN:
Category : Buckingham (England)
Languages : en
Pages : 694
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Book Description
Author: Eric S. Raymond
Publisher: Createspace Independent Publishing Platform
ISBN: 9781548201579
Category :
Languages : en
Pages : 508
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Book Description
This document is a collection of slang terms used by various subcultures of computer hackers. Though some technical material is included for background and flavor, it is not a technical dictionary; what we describe here is the language hackers use among themselves for fun, social communication, and technical debate.
Author: Millar Burrows
Publisher:
ISBN:
Category : Manuscripts, Hebrew
Languages : iw
Pages : 32
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Book Description
Author: John Arkas Hawgood
Publisher:
ISBN:
Category : West (U.S.)
Languages : en
Pages : 490
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Book Description
Includes information on the discovery of gold in California, the Pony Express, missions and missionaries in California, stagecoaches, transcontinental railroad, etc.
Author: Al Stohlman
Publisher:
ISBN: 9781892214997
Category :
Languages : en
Pages :
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Book Description
Shows and explains figure and western floral leather carving (including lots of animals). Features over 60 patterns for billfolds, belts and more. 48 pages.
Author:
Publisher:
ISBN: 9780201658910
Category : Computers
Languages : en
Pages : 376
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Book Description
Explains how to use the multimedia animation program to manage layer speed, transition, create templates, animate backgrounds, and import movies.
Author: Karen Katz
Publisher: Little Simon
ISBN: 9781481442091
Category : Juvenile Fiction
Languages : en
Pages : 0
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Book Description
Vroom vroom! Here come the construction trucks in this new lift-the-flap book from Karen Katz featuring the cutest trucks you’ve ever seen. Rumble! Rumble! Here come the big trucks! Look for bulldozers, dump trucks, and more in this adventurous lift-the-flap book from Karen Katz sure to appeal to any preschooler who loves things that go and things that build. With Karen Katz’s signature bright, striking art, this board book with its sturdy, easy-to-lift flaps is sure to vroom onto bookshelves everywhere.
Author: DK
Publisher: National Geographic Books
ISBN: 146546526X
Category : Juvenile Fiction
Languages : en
Pages : 0
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Book Description
Pop-Up Peekaboo! brings you a wonderfully engaging and all-encompassing baby book, ideal for 0-3 year olds, full of love, learning and so much more! Introducing Pop-Up Peekaboo! I Love You - a wonderfully engaging and interactive pop-up book, complete with simple read-aloud text to catch your toddler’s eye and shape their learning experience like never before. Discover who’s sending Smiley Sheep presents in this adorable baby board book, complete with lift-the-flaps, pop-ups and more! Encompassing fun first words and enchanting adventures, this beautiful baby book encourages literacy in young learners, gently introducing key terms and read-aloud rhymes within its pages of highly-interactive pop-up play! Dive straight in to discover: • Five lift-the-flaps and five interactive pop-ups revealing hidden surprises • Rhyming text and important vocabulary words to encourage early-learning in 0-3 year olds • Eye-catching illustrations bringing the key characters to life • Safe, sturdy board book with rounded edges ideal for little hands Celebrate your child’s curiosity with this chunky and easy-to-hold book, sure to positively influence their memory and motor skills, developing literacy and early-learning, whilst they stay thoroughly entertained, one pop-up peekaboo at a time! The ideal baby book for preschool school learning, Pop-up Peekaboo! I Love You is certain to keep little ones fascinated and engaged time and time again, and proves a must-have volume for parents and children to read aloud together. Why not make your baby’s first Valentine’s Day extra special, with their very own pop-up learning book, complete with cuddly characters and an excellent array of fun first words! Pop-Up Peekaboo I Love You proves the perfect Valentine’s Day gift to treat your toddler this year. Peekaboo! I see you’re looking for more fun-filled adventures! Well, look no further! Pop-Up Peekaboo! is back with a complete collection of over 20 interactive books, so you can enjoy endless hours of pop-up partying. Shortlisted for the Best Preschool Reading Range in The Progressive Preschool Awards 2018, the peekaboo books truly do have something for every young reader to enjoy! Discover all the fascinating stages of a butterfly’s life cycle in Pop Up Peekaboo! Butterfly or travel to a galaxy far away in Pop-Up Peekaboo! Space. Join the peekaboo club today, your next adventure is only one pop-up away!
