Handbook of Microscopy

Handbook of Microscopy PDF Author: Marcel Locquin
Publisher: Butterworth-Heinemann
ISBN: 148316487X
Category : Science
Languages : en
Pages : 335

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Book Description
Handbook of Microscopy is a manual that deals mainly with the basic instruments and techniques used in light microscopy and its biological applications. A large section is devoted to the study of organic matter in microfossils preserved in rocks, in view of its stratigraphic importance in mining and oil prospecting. This text is comprised of six chapters; the first of which introduces the reader to the basic principles as well as to the instruments and techniques used in light microscopy. This book also discusses the microscopes and electronic flashlights for photomicrography, along with the use of monochromatic light, stereological and physicochemical microanalysis, microanalysis by electron microscopy, and microdetermination of physical values. Attention then turns to staining and impregnation and methods of fixation, examination, cutting, and mounting. The remaining chapters focus on the microscopy of topological stains and non-specific cytological stains, with emphasis on special methods used in animal and plant histology and protistology and mycological methods in pathology. This book is written specifically for microscopists.

Handbook of Microscopy

Handbook of Microscopy PDF Author: Marcel Locquin
Publisher: Butterworth-Heinemann
ISBN: 148316487X
Category : Science
Languages : en
Pages : 335

Get Book Here

Book Description
Handbook of Microscopy is a manual that deals mainly with the basic instruments and techniques used in light microscopy and its biological applications. A large section is devoted to the study of organic matter in microfossils preserved in rocks, in view of its stratigraphic importance in mining and oil prospecting. This text is comprised of six chapters; the first of which introduces the reader to the basic principles as well as to the instruments and techniques used in light microscopy. This book also discusses the microscopes and electronic flashlights for photomicrography, along with the use of monochromatic light, stereological and physicochemical microanalysis, microanalysis by electron microscopy, and microdetermination of physical values. Attention then turns to staining and impregnation and methods of fixation, examination, cutting, and mounting. The remaining chapters focus on the microscopy of topological stains and non-specific cytological stains, with emphasis on special methods used in animal and plant histology and protistology and mycological methods in pathology. This book is written specifically for microscopists.

Handbook of Biomedical Nonlinear Optical Microscopy

Handbook of Biomedical Nonlinear Optical Microscopy PDF Author: Barry R. Masters
Publisher: Oxford University Press
ISBN: 0198036825
Category : Science
Languages : en
Pages : 895

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Book Description
The Handbook of Biomedical Nonlinear Optical Microscopy provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections. The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing.

Handbook of Medical Imaging

Handbook of Medical Imaging PDF Author:
Publisher: Academic Press
ISBN: 0080533108
Category : Science
Languages : en
Pages : 983

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Book Description
In recent years, the remarkable advances in medical imaging instruments have increased their use considerably for diagnostics as well as planning and follow-up of treatment. Emerging from the fields of radiology, medical physics and engineering, medical imaging no longer simply deals with the technology and interpretation of radiographic images. The limitless possibilities presented by computer science and technology, coupled with engineering advances in signal processing, optics and nuclear medicine have created the vastly expanded field of medical imaging. The Handbook of Medical Imaging is the first comprehensive compilation of the concepts and techniques used to analyze and manipulate medical images after they have been generated or digitized. The Handbook is organized in six sections that relate to the main functions needed for processing: enhancement, segmentation, quantification, registration, visualization as well as compression storage and telemedicine. * Internationally renowned authors(Johns Hopkins, Harvard, UCLA, Yale, Columbia, UCSF) * Includes imaging and visualization * Contains over 60 pages of stunning, four-color images

Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis

Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis PDF Author: Patrick Echlin
Publisher: Springer Science & Business Media
ISBN: 0387857311
Category : Technology & Engineering
Languages : en
Pages : 329

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Book Description
Scanning electr on microscopy (SEM) and x-ray microanalysis can produce magnified images and in situ chemical information from virtually any type of specimen. The two instruments generally operate in a high vacuum and a very dry environment in order to produce the high energy beam of electrons needed for imaging and analysis. With a few notable exceptions, most specimens destined for study in the SEM are poor conductors and composed of beam sensitive light elements containing variable amounts of water. In the SEM, the imaging system depends on the specimen being sufficiently electrically conductive to ensure that the bulk of the incoming electrons go to ground. The formation of the image depends on collecting the different signals that are scattered as a consequence of the high energy beam interacting with the sample. Backscattered electrons and secondary electrons are generated within the primary beam-sample interactive volume and are the two principal signals used to form images. The backscattered electron coefficient ( ? ) increases with increasing atomic number of the specimen, whereas the secondary electron coefficient ( ? ) is relatively insensitive to atomic number. This fundamental diff- ence in the two signals can have an important effect on the way samples may need to be prepared. The analytical system depends on collecting the x-ray photons that are generated within the sample as a consequence of interaction with the same high energy beam of primary electrons used to produce images.

Handbook of Full-Field Optical Coherence Microscopy

Handbook of Full-Field Optical Coherence Microscopy PDF Author: Arnaud Dubois
Publisher: CRC Press
ISBN: 9814669172
Category : Medical
Languages : en
Pages : 790

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Book Description
Full-field optical coherence microscopy (FF-OCM) is an imaging technique that provides cross-sectional views of the subsurface microstructure of semitransparent objects. The technology is based on low-coherence interference microscopy, which uses an area camera for en face imaging of the full-field illuminated object. FF-OCM benefits from the lateral imaging resolution of optical microscopy along with the capacity of optical axial sectioning at micrometer-scale resolution. The technique can be employed in diverse applications, in particular for non-invasive examination of biological tissues. This handbook is the first to be entirely devoted to FF-OCM. It is organized into four parts with a total of 21 chapters written by recognized experts and major contributors to the field. After a general introduction to FF-OCM, the fundamental characteristics of the technology are analyzed and discussed theoretically. The main technological developments of FF-OCM for improving the image acquisition speed and for endoscopic imaging are presented in part II. Extensions of FF-OCM for image contrast enhancement or functional imaging are reported in part III. The last part of the book provides an overview of possible applications of FF-OCM in medicine, biology, and materials science. A comprehensive compilation of self-contained chapters written by leading experts, this handbook is a definitive guide to the theoretical analyses, technological developments, and applications of FF-OCM. Using the rich information the book is replete with, a wide range of readers, from scientists and physicists to engineers as well as clinicians and biomedical researchers, can get a handle on the latest major advances in FF-OCM.

Handbook of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy PDF Author: James Pawley
Publisher: Springer Science & Business Media
ISBN: 1475753489
Category : Science
Languages : en
Pages : 639

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Book Description
This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.

A Handbook of Medical Microscopy

A Handbook of Medical Microscopy PDF Author: Joseph Gibbons Richardson
Publisher:
ISBN: 9783337708436
Category :
Languages : en
Pages : 340

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Book Description


Handbook of Medical Image Computing and Computer Assisted Intervention

Handbook of Medical Image Computing and Computer Assisted Intervention PDF Author: S. Kevin Zhou
Publisher: Academic Press
ISBN: 0128165863
Category : Computers
Languages : en
Pages : 1074

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Book Description
Handbook of Medical Image Computing and Computer Assisted Intervention presents important advanced methods and state-of-the art research in medical image computing and computer assisted intervention, providing a comprehensive reference on current technical approaches and solutions, while also offering proven algorithms for a variety of essential medical imaging applications. This book is written primarily for university researchers, graduate students and professional practitioners (assuming an elementary level of linear algebra, probability and statistics, and signal processing) working on medical image computing and computer assisted intervention. - Presents the key research challenges in medical image computing and computer-assisted intervention - Written by leading authorities of the Medical Image Computing and Computer Assisted Intervention (MICCAI) Society - Contains state-of-the-art technical approaches to key challenges - Demonstrates proven algorithms for a whole range of essential medical imaging applications - Includes source codes for use in a plug-and-play manner - Embraces future directions in the fields of medical image computing and computer-assisted intervention

Fundamentals of Light Microscopy and Electronic Imaging

Fundamentals of Light Microscopy and Electronic Imaging PDF Author: Douglas B. Murphy
Publisher:
ISBN:
Category :
Languages : en
Pages : 538

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Book Description


Handbook of Cryo-Preparation Methods for Electron Microscopy

Handbook of Cryo-Preparation Methods for Electron Microscopy PDF Author: Annie Cavalier
Publisher: CRC Press
ISBN: 9780849372278
Category : Science
Languages : en
Pages : 0

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Book Description
While new discoveries have led to much dramatic growth in cryo-electron microscopy, researchers will never be able to take full advantage if they lack access to the details that make these techniques understandable and applicable. The Handbook of Cryo-Preparation Methods for Electron Microscopy provides researchers with a complete reference that will show them how to equip their labs with the right materials and methods to take full advantage of the latest advances. This pioneering work brings together a group of internationally renowned researchers, some the very inventors of the methods they describe, to share their knowledge and recipes. Taking care to explain the history behind the techniques and to demonstrate their use, this book presents the latest theory, principles, and protocols supplemented by hundreds of illustrations. Contributions to this handbook – Ö Describe cryo-methods aimed at perfect preservation for fine structural analysis Ö Teach how to arrest physiological processes by cryo-fixation Ö Reveal the secrets for high-resolution snapshots of life by CEMOVIS Ö Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography Ö Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and molecules Ö Present hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geography Ö Illustrate freeze-fracture, cryo-ultramicrotomy, and resin embedding Ö Highlight the Tokuyasu method and the new rehydration technique for highly efficient immunolabeling Ö Include summary tables to compare and obtain appropriate criteria Includes a wealth of immediate reference material for daily use With clear, step-by-step recipes and lists of tools, ingredients, and suppliers, this handbookprovides researchers with the knowledge and techniques to adopt the latest cryo-methods to their current research.