Author: Oliver Lowry
Publisher: Elsevier
ISBN: 0323156711
Category : Science
Languages : en
Pages : 304
Get Book Here
Book Description
A Flexible System of Enzymatic Analysis is a multipurpose manual of laboratory methods that offers a systematic scheme for the analysis of biological materials from the level of the wholeorgan down to the single cell and beyond. It intends to guide the development of methods, the refinement of old ones, and the adaptation in general of methods to almost any scale of sensitivity. This manual is organized into three parts: a general section, one on quantitative histochemistry, and an appendix containing information that may be useful to have at the bench. The general section is comprised of nine chapters that focus on properties of the pyridine nucleotides, kinetics, and glassware, as well as on improvement, modification, adaptation, trouble shooting, and development of methods. This part also describes the preparation of tissues for analysis, the enzymatic cycling methods, and a compendium of 36 metabolite assays. The quantitative histochemistry section is comprised of four chapters that include information on the preparation of frozen-dried material and dissection of samples for analysis; the fishpole balance for weighing samples; and the generalities of analysis with emphasis on the ""oil well technique."" This book will be useful to novices as well as experts who are familiar with other analytical styles.
Author: Janet V. Passonneau
Publisher: Springer Science & Business Media
ISBN: 1603274073
Category : Science
Languages : en
Pages : 403
Get Book Here
Book Description
Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity—all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step.
Author: V. M. Andreoli
Publisher: Springer Science & Business Media
ISBN: 3642885160
Category : Medical
Languages : en
Pages : 193
Get Book Here
Book Description
V M. ANDREOLl, A. AGNOLI, and C. FAZIO The transfer of a methyl group from a donor to an acceptor compound is a fundamental biochemie al process long since known to biologists; the process is involved, for instance, in the metabolism of porphyrins, nuc1eic acids, and fatty acids. Only recently, however, did transmethylation processes reveal their decisive role in CNS biochemistry - namely with the discovery that such processes are linked with the biogenie amines that have been conc1usively identified as the chemical mediators of neu ronal transmission and, more broadly, of behavior. The first suggestion that transmethylation processes might be involved in the origina tion of certain mental diseases came from Harley-Mason (1952), who noticed that many of the hallucinogenic substances known at that time contained methyl radicals, and par ticularly that mescaline represented the product of O-methylation of dopamine in posi tions 3, 4, and 5. This hypothesis was put forward when the O-methylation of catechol amines by catechol-O-methyltransferases had not yet been described. Harley-Mason further proposed that in the living organism the process of O-methyla tion might follow a "deviant" metabolie pathway, and that the accumulation of abnor mal methylated metabolites endowed with hallucinogenic properties might be responsi ble for the implementation of some mental diseases, notably schizophrenia. He also called attention to a substance, 3,4-dimethoxyphenylethylamine (3,4-DMPEA) as a possible psychotogenic moleeule, on the strength of its capacity for producing cata tonia in experimental animals
Author: Janet R. Stein-Taylor
Publisher: Cambridge University Press
ISBN: 9780521200493
Category : Algae
Languages : en
Pages : 536
Get Book Here
Book Description
Isolation and purification; General equipment and methods; Special culture methods; Growth measurements; Bioassay.
Author: Leo M.L. Nollet
Publisher: CRC Press
ISBN: 142004530X
Category : Technology & Engineering
Languages : en
Pages : 990
Get Book Here
Book Description
In today's nutrition-conscious society, there is a growing awareness among meat scientists and consumers about the importance of the essential amino acids, vitamins, and minerals found in muscle foods. Handbook of Muscle Foods Analysis provides a comprehensive overview and description of the analytical techniques and application methodologies for t
Author: R. W. Frei
Publisher: Springer Science & Business Media
ISBN: 1468442023
Category : Science
Languages : en
Pages : 309
Get Book Here
Book Description
With the second volume in this senes we have continued the theme of Volume 1 and expanded more generally into separation and continuous flow techniques. The first chapter gives an account on flow injection analysis. Thi~ technique has gained considerable interest in the recent past and offers many facets of the use of chemistry in automated analysis procedures. One of these facets is certainly also the use of ion-pairing reactions, and we have been fortunate to get two well-known experts to treat this subject. The use of ion-pairing techniques is being discussed from a batch extraction (sample handling) point of view as well as for flow injection and chromato graphic purposes. Immobilized enzymes are another area of major attention and their multidirectional use in analytical chemistry is illuminated in Chapter 3 with special emphasis on their use in connection with liquid chromatography. Needless to say, similar techniques have also found their way into con tinuous-flow methodology (Auto-Analyzers) and many of the aspects dis cussed in Chapter 3 are adaptable to this end. The need and recent urgency for separation of optical isomers in many areas, particularly pharmaceutical and clinical analysis, has spurred a flurry of activity in this special section of chromatography. The current status of the field of optical isomer separation is competently reviewed in Chapter 4. Again this is a typical area wherein it is the proper use of chemistry that finally delivers a solution. The last two chapters deal with precolumn or prechromatographic derivatization techniques.
Author: Edward T. Maggio
Publisher: CRC Press
ISBN: 1351080296
Category : Health & Fitness
Languages : en
Pages : 306
Get Book Here
Book Description
The purpose of this book is to focus attention on some of these ideas and concepts. In doing so, it has captured a glimpse of the past and it attempts a projection of the future, but mostly it reveals an overview of the field as it exists as the present time. It aims to serve to spawn further growth in ideas and encourage applications to increasingly broader segments of both clinical and general analytical chemistry fields.
Author: Thomas C. Jones
Publisher: Springer Science & Business Media
ISBN: 3642969100
Category : Medical
Languages : en
Pages : 896
Get Book Here
Book Description
The International Life Sciences Institute (ILSI) was creat ed to promote cooperative efforts toward solving critical health and safety questions involving foods, drugs, cosmet ics, chemicals, and other aspects of the environment. The Officers and Trustees believe that questions regarding health and safety are best resolved when government and industry rely on scientific investigations, analyses, and reviews by independent experts. Further, the scientific aspects of an issue should be examined and discussed on an international basis, separate from the political concerns of individual companies. ILSI is pleased to sponsor this set of monographs on the pathology of laboratory animals. This project will be use ful in improving the scientific basis for the application of pathologic techniques to health and safety evaluation of substances in our environment. The world wide distribu tion of the authors, editors, and Editorial Board who are creating these monographs strengthens the expectation that international communication and cooperation will al so be strengthened.
Author: K.-O. Habermehl
Publisher: Springer Science & Business Media
ISBN: 364269943X
Category : Medical
Languages : en
Pages : 745
Get Book Here
Book Description
Rapid progress in molecular biology, genetic engineering, and basic research in immunology has opened up new possibilities for application to diagnostic procedures and to clinical research. In a short period a new era of diagnosis dawned, covering nearly all fields of microbiology, immunology, and food technology. In consequence of this rapid development, scientists of many disciplines are involved studying infections of humans, animals, and plants or working in technical microbiology. The application of the newest findings of basic research to diagnostic work and to clinical research covers nearly all fields of microbiology and immunology. Moreover, it underlines the close relationship between diagnosis, therapy, and epidemiology. An outstanding example of these connections is given by the recent development of hepatitis B vaccine. The discovery and identification of a non cultivable agent by physicochemical and immunological methods were the heralds of a new era in the prevention of infectious diseases. This book provides an up-to-date, comprehensive review of developments and future aspects in various fields. I am convinced that the authors have succeeded in furnishing a large variety of new ideas and possibilities. K.-O. HABERMEHL Contents Time Realities in the Evaluation of Vaccines for Safety and Efficacy The Evaluation of Vaccines M. R. HILLEMAN . . . . .
Author: David Evered
Publisher: John Wiley & Sons
ISBN: 0470718242
Category : Science
Languages : en
Pages : 192
Get Book Here
Book Description
The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.
