3' Processing of Messenger RNA in the Yeast Saccharomyces Cerevisiae PDF Download
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Author: Christoph Mathias Egli
Publisher:
ISBN:
Category : Genetic transcription
Languages : en
Pages : 124
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Book Description
Author: Christoph Mathias Egli
Publisher:
ISBN:
Category : Genetic transcription
Languages : en
Pages : 124
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Book Description
Author: Stefan Irniger
Publisher:
ISBN:
Category :
Languages : en
Pages : 144
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Author: Christine Elizabeth Brown
Publisher:
ISBN:
Category :
Languages : en
Pages : 376
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Author: Susan Campbell
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Steven Bruce Baim
Publisher:
ISBN:
Category :
Languages : en
Pages : 200
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Author: Tyson A. Clark
Publisher:
ISBN:
Category : Eukaryotic cells
Languages : en
Pages : 422
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Book Description
Author: Jeffrey N. Strathern
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 776
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Author:
Publisher: Academic Press
ISBN: 0128235748
Category : Science
Languages : en
Pages : 502
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Book Description
mRNA 3’ End Processing and Stability, Volume 655 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics. Each chapter is written by an international board of authors. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Updated release includes the latest information on mRNA 3' End Processing and Stability
Author: Wei Hou
Publisher:
ISBN:
Category :
Languages : en
Pages : 344
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Author: Weifei Luo
Publisher:
ISBN: 9781109838848
Category :
Languages : en
Pages : 145
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Book Description
Transcription termination by RNA polymerase II (pol II) is a process that involves the release of polymerase and the RNA from the transcription elongation complex. In eukaryotes, transcription termination is tightly coupled to mRNA 3' end processing (cleavage/polyadenylation) largely via the C-terminal domain (CTD) of pol II. The torpedo model for pol II transcription termination proposes that a 5'-3' RNA exonuclease enters at the poly (A) cleavage site, degrades the 3' nascent RNA and eventually displaces polymerase from the DNA. We directly demonstrated that two exonucleases, Rat1 and Xrn1, both contribute to co-transcriptional degradation of nascent RNA but this degradation is not sufficient to cause polymerase release. Instead, Rat1 functions in both 3' end processing and termination by enhancing recruitment of 3' end processing factors. In addition, the cleavage factor Pcf11 reciprocally aids in recruitment of Rat1 to the elongation complex. How 3' end cleavage of pre-mRNA is involved in termination is still not well understood. We introduced a variant of hepatitis delta ribozyme either in the coding region or 3' flanking region of ADI-4 gene and investigated whether severing the nascent by the self-cleaving ribozyme could affect transcription termination. We showed that ribozyme cleavage within the coding region of ADH4 stimulates premature termination and dramatically enhances the recruitment of Rat1, Pcf11 and Rna15 near the ribozyme sequence although the ribozyme inserted downstream of the ADH4 poly (A) site did not affect normal termination and replacement of the ADH4 3' UTR by the ribozyme did not restore termination in the region immediately downstream. We also demonstrated that Ctk1, Ess1 and Spt5 are required for transcription termination; and Ess1 is essential for proper recruitment of Spt5 and Pcf11 of the CFIA complex at the 3' end of a gene. These observations imply that both the CTD phosphorylation and conformational modification are important to stimulate efficient termination. In summary, the results presented in this thesis suggest a unified model for pol II transcription termination: multiple contacts among the polymerase, Rat1, CFIA complex and the RNA function together to ensure efficient termination.
