X-ray Microanalysis in Biology

X-ray Microanalysis in Biology PDF Author: David C. Sigee
Publisher: Cambridge University Press
ISBN: 9780521415309
Category : Medical
Languages : en
Pages : 356

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Book Description
This book describes an integrated approach to the use of X-ray microanalysis in biology.

X-ray Microanalysis in Biology

X-ray Microanalysis in Biology PDF Author: David C. Sigee
Publisher: Cambridge University Press
ISBN: 9780521415309
Category : Medical
Languages : en
Pages : 356

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Book Description
This book describes an integrated approach to the use of X-ray microanalysis in biology.

X-Ray Optics and X-Ray Microanalysis

X-Ray Optics and X-Ray Microanalysis PDF Author: H. H. Pattee
Publisher: Elsevier
ISBN: 1483277038
Category : Science
Languages : en
Pages : 641

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Book Description
X-ray Optics and X-ray Microanalysis covers the proceedings of the Symposium on X-ray Optics and X-ray Microanalysis, held at Stanford University on August 22-24, 1962. The book focuses on X-ray microscopy, microradiography, radiation and irradiation, and X-ray microanalysis. The selection first offers information on the methods of X-ray microscopy and X-ray absorption microanalysis. Discussions focus on X-ray scanning microscopy, contact microradiography, point projection microscopy, and total dry-weight determinations. The text then takes a look at X-ray microanalysis in biology and medicine; electron microscopic enlargements of X-ray absorption micrographs; and automation in microradiography. The publication examines the production of Fresnel zone plates for extreme ultraviolet and soft X radiation; quantitative microradiographic studies of human epidermis; and irradiation effect on total organic nerve-cell material determined by integrating X-ray absorption. The manuscript then reviews the calculation of fluorescence excited by characteristic radiation in the X-ray microanalyzer and the method for calculating the absorption correction in electron-probe microanalysis. The selection is a valuable reference for readers interested in X-ray technology.

Basic Methods in Biological X-ray Microanalysis

Basic Methods in Biological X-ray Microanalysis PDF Author: Godfried M. Roomans
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 292

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Book Description


Microautoradiography and Electron Probe Analysis

Microautoradiography and Electron Probe Analysis PDF Author: U. Lüttge
Publisher: Springer Science & Business Media
ISBN: 3642874967
Category : Science
Languages : en
Pages : 245

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Book Description
In biological literature, several definitions of quantitative autoradio graphy are given. The term is defined as either the determination and com parison of the density of silver grains above various structures or under varying conditions, or the determination of absolute quantities of radio activity. In both these cases, photometric measurement serves for more rapid and more exact evaluation of grain densities than would be possible by visual counting of the grains. The equipment generally used for the photometric measurement of silver grains consists of a microscope, a photocell, an electronic amplifier system and a display unit. Grains can be made accessible to photometric evaluation by various kinds of microscopic illumination: 1. Substage bright-field illumination. 2. Substage dark-field illumination. 3. Incident dark-field illumination. 4. Vertical bright-field illumination. With all these types of illumination, the relationship between the luminous flux I absorbed by the film, scattered into the objective and reflected or diffracted, and the flux 10 which is not affected by the film is used as a measure of grain density. Since these are differential measurements, the light beam I transmitted by the film is in itself a measure of grain density if the luminous flux 10 incident on the grains is kept constant. This approach has been used in a large number of measuring setups.

X-ray Microanalysis in Biology and Medicine

X-ray Microanalysis in Biology and Medicine PDF Author: Arne Engström
Publisher:
ISBN:
Category : Microradiography
Languages : en
Pages : 108

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Book Description


Biomedical Applications of Microprobe Analysis

Biomedical Applications of Microprobe Analysis PDF Author: Peter Ingram
Publisher: Academic Press
ISBN: 0080524567
Category : Science
Languages : en
Pages : 573

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Book Description
Biomedical Applications of Microprobe Analysis is a combination reference/laboratory manual for the use of microprobe analysis in both clinical diagnostic and research settings. Also called microchemical microscopy, microprobe analysis uses high-energy bombardment of cells and tissue, in combination with high resolution EM or confocal microscopy to provide a profile of the ion, metal, and mineral concentrations present in a sample. This allows insight into the physiology and pathophysiology of a wide variety of cells and tissues.This book describes methods for obtaining detailed information about the identity and composition of particles too small to be seen with the naked eye and describes how this information can be useful in diagnostic and biomedical research. - Up-to-date review of electron microprobe analysis - Detailed descriptions of sample preparation techniques - Recent technologies including confocal microscopy, infrared microspectroscopy, and laser raman spectroscopy - Over 100 illustrations with numerous specific applications - Contributions by world-renowned experts in the field - Brief summary of highlights precedes each chapter

Electron Microscopy Methods and Protocols

Electron Microscopy Methods and Protocols PDF Author: M. A. Nasser Hajibagheri
Publisher: Springer Science & Business Media
ISBN: 1592592015
Category : Science
Languages : en
Pages : 292

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Book Description
Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).

Microprobe Analysis in Biology and Medicine

Microprobe Analysis in Biology and Medicine PDF Author: Patrick Echlin
Publisher:
ISBN:
Category : Biology
Languages : en
Pages : 400

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Book Description


Cells and Tissues

Cells and Tissues PDF Author: Arthur W. Pollister
Publisher: Academic Press
ISBN: 1483270777
Category : Science
Languages : en
Pages : 423

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Book Description
Physical Techniques in Biological Research, Volume III, Part A: Cells and Tissues focuses on improvements and innovations in physical techniques involved in biological research on cells and tissues. The selection first offers information on phase contrast and interference microscopy in cytology and birefringence and dichroism of cells and tissues. Topics include observational methods and results, quantitative techniques, nerve and muscle fibers, chloroplasts, cytoplasm, and nucleus. The book then examines X-ray microscopy and X-ray absorption analysis, including theoretical considerations of X-ray microscopy of biological materials and absorption and production of X-rays. The publication ponders on microtomy and manometric techniques for single cells and electron microscopy of microorganisms. Discussions focus on Cartesian diver respirometers, capillary respirometers, preliminary preparation of materials for drying, and techniques for studying dried specimens. The selection is highly recommended for students and readers interested in the physical techniques used in biological research.

Examining the Submicron World

Examining the Submicron World PDF Author: Ralph Feder
Publisher: Springer Science & Business Media
ISBN: 146132209X
Category : Social Science
Languages : en
Pages : 381

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Book Description
An Institute like ours cannot help but lend credence to the notion of the late Derek J. de Solla Price of Yale University that "the scientific revolution was largely the improvement, invention and use of a series of instruments . . . . that expanded the reach of science in innumerable directions". Most of science today and in years gone by depends on the experimental observation of struc ture on the small scale with microscopes, and on the large scale with telescopes. The first instruments to expand the observational range of the human eye were simple optical systems, designed in the case of microscopes and telescopes to magnify the image. The big breakthrough in the 17th century was not when Galileo first turned his telescope to the heavens, but when improvements in lens-grinding techniques allowed eyeglass makers to make the first telescope. Early microscopy revealed new and previously unsuspected microstruc tures in biological and non-biological materials and thus helped to enlarge on the understanding of the relationship between structure and properties. The natural inclination of all microscopists, the desire to observe ever smaller structures, was satisfied by the construction of higher quality optical systems which reduced the aberrations limiting the usable magnification. The modem optical microscope is the result of this evolution in design and construction, and it can be operated easily to achieve close to the theoretical resolution.