Molecular Biology of the Cell PDF Download
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Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Book Description
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Book Description
Author: Susan Buchanan
Publisher: Humana
ISBN: 9781493928705
Category : Science
Languages : en
Pages : 0
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Book Description
This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.
Author: Raffaele Ieva
Publisher: Springer Nature
ISBN: 1071637347
Category :
Languages : en
Pages : 388
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Book Description
Author: Marco Fioroni
Publisher: Springer Science & Business Media
ISBN: 940077429X
Category : Science
Languages : en
Pages : 178
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Book Description
ß-barrel outer membrane channel proteins (OMP) are useful as robust and flexible models or components in nanotechnology. Over the last decade biotechnological techniques allowed to expand the natural characteristics of OMPs by modifying their geometry and properties. The present book is oriented towards a broad group of readers including graduate students and advanced researchers. It gives a general introduction to the field of OMP based nano-component development as well as the state of the art of the involved research. On the example of the E. coli FhuA the transformation of an OMP into a tailored nano-channel will be outlined. An exhaustive description of the scientific strategy, including protein selection, analytical methods and “in-silico” tools to support the planning of protein modifications for a targeted application, consideration on the production of a custom made OMP, and an overview on technological applications including membrane/polymersome technology, will be provided.
Author: Jos A.F. Op den Kamp
Publisher: Springer Science & Business Media
ISBN: 3642731848
Category : Science
Languages : en
Pages : 474
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Book Description
Many individual aspects of the dynamics and assembly of biological membranes have been studied in great detail. Cell biological approaches, advanced genetics, biophysics and biochemistry have greatly contributed to an increase in our knowledge in this field.lt is obvious however, that the three major membrane constituents - lipids, proteins and carbohydrates- are studied, in most cases separately and that a coherent overview of the various aspects of membrane biogenesis is not readily available. The NATO Advanced Study Institute on "New Perspectives in the Dynamics of Assembly of Biomembranes" intended to provide such an overview: it was set up to teach students and specialists the achievements obtained in the various research areas and to try and integrate the numerous aspects of membrane assembly into a coherent framework. The articles in here reflect this. Statting with detailed contributions on phospholipid structure, dynamics, organization and biogenesis, an up to date overview of the basic, lipidic backbone of biomembranes is given. Extensive progress is made in the research on membrane protein biosynthesis. In particular the post- and co-translational modification processes of proteins, the mechanisms of protein translocation and the sorting mechanisms which are necessary to direct proteins to their final, intra - or extracellular destination have been characterized in detail. Modern genetic approaches were indispensable in this research area: gene cloning, hybrid protein construction, site directed mutagenesis and sequencing techniques elucidated many functional aspects of specific nucleic acid and amino acid sequences.
Author: Gunnar von Heijne
Publisher: R. G. Landes
ISBN:
Category : Science
Languages : en
Pages : 300
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Book Description
Author: Laszlo Patthy
Publisher: John Wiley & Sons
ISBN: 1444308882
Category : Science
Languages : en
Pages : 392
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Book Description
This book provides an up-to-date summary of the principles of protein evolution and discusses both the methods available to analyze the evolutionary history of proteins as well as those for predicting their structure-function relationships. Includes a significantly expanded chapter on genome evolution to cover genomes of model organisms sequenced since the completion of the first edition, and organelle genome evolution Retains its reader-friendly, accessible style and organization Contains an updated glossary and new references, including a list of online reference sites
Author: Daniel Hsieh
Publisher:
ISBN:
Category : Bacterial proteins
Languages : en
Pages : 172
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Book Description
Outer membrane proteins (OMPs) perform a range of important functions in the cell biology of Gram-negative bacteria, mitochondria and chloroplasts. These functions include biogenesis, virulence, signal transduction, nutrient transport and apoptosis. In contrast to their inner membrane counterparts, OMPs have been more difficult to study due to the relative paucity of crystal structures. Although outer membrane proteins have been characterized and studied extensively by various structural and biophysical methods, our understanding of their folding, insertion and oligomerization is far behind that of inner membrane proteins. The goal of this study is to elucidate the folding and insertion mechanism of these transmembrane [beta]-barrel proteins (TMBs) and ultimately to provide guidelines for computationally designing OMP sequences that fold and insert efficiently. Using a subset of amino acids from thirty-five outer membrane proteins from Gram-negative bacteria and mitochondria, a propensity vs depth in membrane profile for each residue was derived. Although results indicate similar trends between amino acids of inner and outer membrane proteins, there are also differences that can be explained by differences in factors such as environment, secondary structural preferences, and folding/insertion pathway. The propensity profiles were converted into energies of insertion as a function of depth from the center of the membrane. This thesis explores the many ways of using the statistical potential to answer questions about OMP folding, insertion and oligomerization that could not have been framed due to the experimental limitations. We conclude with a discussion of ways to improve our potential, including the assumption of asymmetry of the lipid bilayer as well as incorporating homology model building.
Author: Vivien Yeh
Publisher: Springer
ISBN: 9811312389
Category : Science
Languages : en
Pages : 151
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Book Description
This book focuses on the study of how the properties of nanodiscs, such as lipid composition and size, influence the function of the embedding integral membrane protein, bacteriorhodopsin. The author performed systematic studies to show that the lipid composition and the charge of the hydrophobic head and the structure of hydrophilic tails affect the photocycle pathway of bacteriorhodopsin, which is closely associated with its proton-pumping activity. Furthermore, the author demonstrated a highly efficient method for extracting membrane proteins directly from the biological membrane, preserving protein conformation, function and essential native lipids. This book demonstrates optimization and sample preparation, and presents practical methods of preparing membrane protein-embedded nanodisc samples for biophysical studies, which benefit structural and functional studies in the field of membrane protein characterization, both.
Author: J.-M. Ghuysen
Publisher: Elsevier
ISBN: 0080860877
Category : Science
Languages : en
Pages : 607
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Book Description
Studies of the bacterial cell wall emerged as a new field of research in the early 1950s, and has flourished in a multitude of directions. This excellent book provides an integrated collection of contributions forming a fundamental reference for researchers and of general use to teachers, advanced students in the life sciences, and all scientists in bacterial cell wall research. Chapters include topics such as: Peptidoglycan, an essential constituent of bacterial endospores; Teichoic and teichuronic acids, lipoteichoic acids, lipoglycans, neural complex polysaccharides and several specialized proteins are frequently unique wall-associated components of Gram-positive bacteria; Bacterial cells evolving signal transduction pathways; Underlying mechanisms of bacterial resistance to antibiotics.
