Translation Initiation: Reconstituted Systems and Biophysical Methods

Translation Initiation: Reconstituted Systems and Biophysical Methods PDF Author:
Publisher: Elsevier
ISBN: 0080553184
Category : Science
Languages : en
Pages : 510

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Book Description
For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the second of three on the topic of Translation Initiation includes articles written by leaders in the field.

Translation Initiation: Reconstituted Systems and Biophysical Methods

Translation Initiation: Reconstituted Systems and Biophysical Methods PDF Author:
Publisher: Elsevier
ISBN: 0080553184
Category : Science
Languages : en
Pages : 510

Get Book Here

Book Description
For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the second of three on the topic of Translation Initiation includes articles written by leaders in the field.

Guide to Techniques in Mouse Development, Part B

Guide to Techniques in Mouse Development, Part B PDF Author: Paul Wassarman
Publisher: Academic Press
ISBN: 0123848814
Category : Science
Languages : en
Pages : 637

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Book Description
This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development - Update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993 - Comprehensively covers: - new techniques for the cryopreservation of gametes and embryos - production of transgenic and null (knockout) animals (use of ES cells) - generation of conditional/inducible mutant animals, use of gene-trap mutagenesis - analysis of allele-specific expression, use of new reporter constructs - humanizing of transgenic animals - transcript profiling of mouse development - imaging of mouse development - rederivation of animals and use of mouse genomics

Guide to Techniques in Mouse Development, Part A

Guide to Techniques in Mouse Development, Part A PDF Author:
Publisher: Academic Press
ISBN: 0080887783
Category : Science
Languages : en
Pages : 539

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Book Description
Guide to Techniques in Mouse Development, Part A comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. - Update of volume 225 of Methods in Enzymology, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993 - Covers new technologies and methodologies, including: - new techniques for the cryopreservation of gametes and embryos - production of transgenic and null (knockout) animals (use of ES cells) - generation of conditional/inducible mutant animals - use of gene-trap mutagenesis - analysis of allele-specific expresion - use of new reporter constructs - humanizing of transgenic animals - transcript profiling of mouse development - imaging of mouse development - rederivation of animals and use of mouse genomics

MicroRNA Methods

MicroRNA Methods PDF Author: John J. Rossi
Publisher: Academic Press
ISBN: 0080551394
Category : Science
Languages : en
Pages : 302

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Book Description
MicroRNAs (miRNA) are tiny bits of genetic material that were unknown nearly 10 years ago but now represent an exciting field of study in biology. Upon their discovery, researchers revealed for the first time a new mechanism by which microRNA can stop the function of messenger RNA (mRNA) by literally cutting it in half, interfering with the normal function of specific messenger RNAs in gene expression. This "expression" of genes that code for essential proteins is essentially what controls whether a cell turns into a liver, lung, or brain cell, for example. Understanding what activates this process – or stops it – is a key to understanding the biological process and builds a foundation for advances in medicine and other fields. This volume in Methods in Enzymology presents valuable methods for studying MicroRNA, with three sections covering identification of MicroRNAs and their targets; MicroRNA expression, maturation and functional analysis; and MicroRNAs and disease.

Synthetic Biology, Part A

Synthetic Biology, Part A PDF Author: Chris Voigt
Publisher: Academic Press
ISBN: 0123850762
Category : Science
Languages : en
Pages : 733

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Book Description
Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation. - Encompasses a variety of different approaches, methodologies and disciplines - Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation

Synthetic Biology

Synthetic Biology PDF Author: Christopher A. Voigt
Publisher: Academic Press
ISBN: 0123850754
Category : Bioengineering
Languages : en
Pages : 734

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Book Description
Synthetic biology encompasses a variety of different approaches, methodologies and disciplines and many different definitions exist. This volume covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation.

Lipidomics and Bioactive Lipids: Specialized Analytical Methods and Lipids in Disease

Lipidomics and Bioactive Lipids: Specialized Analytical Methods and Lipids in Disease PDF Author:
Publisher: Elsevier
ISBN: 0080560652
Category : Science
Languages : en
Pages : 292

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Book Description
This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Liquid chromatography mass spectrometry for quantifying plasma lysophospholipids: potential biomarkers for cancer diagnosis; Measurement of eicosanoids in cancer tissues; Noninvasive Assessment of the Role of Cyclooxygenases in Cardiovascular HealthA Detailed HPLC/MS/MS Method; Lipidomics in Diabetes and the Metabolic Syndrome; LC-MS-MS Analysis of Neutral Eicosanoids; Quantification Of F2-Isoprostanes In Biological Fluids And Tissues As A Measure Of Oxidant Stress; Measurement of Products of Docosahexaenoic Acid Peroxidation, Neuroprostanes, and Neurofurans; Enantiomeric separation of hydroxy and hydroperoxy eicosanoids by chiral column chromatography; Targeted Chiral Lipidomics Analysis by Liquid Chromatography Electron Capture Atmospheric Pressure Chemical Ionization Mass Spectrometry (LC-ECAPCI/MS); Shotgun Lipidomics by Tandem Mass Spectrometry under Data-Dependent Acquisition Control; Identification of Intact Lipid Peroxides by Ag+ Coordination Ionspray Mass Spectrometry (CIS-MS); Quantification of Cardiolipin by Liquid Chromatography Electrospray Ionization Mass Spectrometry.

Oxygen Biology and Hypoxia

Oxygen Biology and Hypoxia PDF Author:
Publisher: Academic Press
ISBN: 0080554865
Category : Science
Languages : en
Pages : 592

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Book Description
For over fifty years the Methods in Enzymology series has been the critically acclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume features articles on the topic of oxygen biology and hypoxia.

Synthetic Biology, Part B

Synthetic Biology, Part B PDF Author: Chris Voigt
Publisher: Academic Press
ISBN: 0123851211
Category : Science
Languages : en
Pages : 556

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Book Description
Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation. - Encompasses a variety of different approaches, methodologies and disciplines - Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation

Biology of Serpins

Biology of Serpins PDF Author: James Whisstock
Publisher: Academic Press
ISBN: 0123864720
Category : Science
Languages : en
Pages : 461

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Book Description
Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. The acronym serpin was originally coined because many serpins inhibit chymotrypsin-like serine proteases. This volume of Methods in Ezymology is split into 2 parts and comprehensively covers the subject.