Plant Tissue Culture PDF Download
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Author: Roberta H. Smith
Publisher: Gulf Professional Publishing
ISBN: 9780126503425
Category : Nature
Languages : en
Pages : 260
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Book Description
This manual provides laboratory exercises in plant tissue culture which demonstrate major educational concepts. It includes sections on scheduling and interrelationships of exercises, tissue culture setup, supplies and media.
Author: Roberta H. Smith
Publisher: Gulf Professional Publishing
ISBN: 9780126503425
Category : Nature
Languages : en
Pages : 260
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Book Description
This manual provides laboratory exercises in plant tissue culture which demonstrate major educational concepts. It includes sections on scheduling and interrelationships of exercises, tissue culture setup, supplies and media.
Author: M. K. Razdan
Publisher: Science Publishers
ISBN: 9781578082377
Category : Science
Languages : en
Pages : 394
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Book Description
Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid production; In vitro pollination and fertilization; Zygotic embryo culture; Somatic hybridisation and cybridisation; Genetic transformation; Somaclonal and gametoclonal variant selection; Application to horticulture and forestry; Production of disease-free plants; clonal propagation; General applications; Industrial applications: secondary metabolite production; Germplasm conservation.
Author: Paul F. Jr. Kruse
Publisher: Elsevier
ISBN: 0323142079
Category : Science
Languages : en
Pages : 897
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Book Description
Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.
Author: John H. Dodds
Publisher: International Potato Center
ISBN: 9780521315166
Category : Science
Languages : en
Pages : 260
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Book Description
The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.
Author: Robert N. Trigiano
Publisher: CRC Press
ISBN: 1439896143
Category : Medical
Languages : en
Pages : 604
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Book Description
Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnol
Author: C Vago
Publisher: Academic Press
ISBN: 1483266923
Category : Science
Languages : en
Pages : 430
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Book Description
Invertebrate Tissue Culture, Volume II presents the organ cultures of various invertebrates. This book discusses the use of cell and organ cultures in genetics, physiology, and pathology. Organized into three parts encompassing 11 chapters, this volume begins with an overview of the factors necessary for the normal development of embryonic or imaginal rudiments. This text then examines the organ culture technique, which is a means of studying the correlations that may exist between various organs as they can be made to act on one another in the same medium independently of any other factor. Other chapters consider a detailed analysis of the utilization of organ culture in pathology. This book discusses as well the three major successive aspects of mollusk organ cultures. The final chapter provides information on cell lines from 35 species of insects from various orders and from one species of mollusk. This book is a valuable resource for biologists, pathologists, and physiologists.
Author: Kenneth C. Torres
Publisher: Springer Science & Business Media
ISBN: 1461597560
Category : Technology & Engineering
Languages : en
Pages : 282
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Book Description
This book was written for those individuals who are concerned about the techniques and practices of plant cell cultures for horticultural crops. It was designed to serve as a text and reference for students and professionals in ornamental horticulture, fruit and vegetable crop pro duction, botany, forestry, and other areas of plant science. Research during the last twenty-five years in the area of plant tissue culture has led to many developments and changes in this field. Al though the techniques involved in the manipulation of plant tissue culture are now relatively straightforward, the presentation of these techniques in a short volume for the beginner in the field is generally unavailable. In addition to describing the techniques for establishment and manipulation of specific species, several chapters in this book also provide a brief, general review of important cultural parameters. Spe cific protocols and laboratory procedures may also be found in the appendix. I hope that this presentation of information will be helpful to those individuals wanting to apply plant tissue culture techniques for horticultural crops.
Author: Jennie P. Mather
Publisher: Springer Science & Business Media
ISBN: 0585275718
Category : Science
Languages : en
Pages : 247
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Book Description
It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.
Author: Jun Mitsuhashi
Publisher: Springer Science & Business Media
ISBN: 4431678751
Category : Science
Languages : en
Pages : 435
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Book Description
I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow ing cells from pupal ovaries of the emperor gum moth, Antheraea euca lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul tured. The vertebrate tissue culture methods gave me some ideas for pre paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec ond, I had to examine various culture media, especially growth-promot ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials? How could I remove tissues from a tiny insect? How many tissues should I pool in order to set up one culture? I had to find out the answers. Naturally, it took a lot of time.
Author: E. N. Willmer
Publisher: Elsevier
ISBN: 1483223000
Category : Science
Languages : en
Pages : 805
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Book Description
Cells and Tissues in Culture: Methods, Biology and Physiology, Volume 1 covers the general fields of tissue culture, including an evaluation of its technique, effects, and contributions to biology. This book focuses on the three methods of culture—tissue culture, cell culture, and organ culture.Other topics include the design of complete synthetic media, possible evolution of the cell types, and energy relationships in growing and stationary cells. The RNA synthesis in cell cultures, culture of amphibian embryonic anlage, action of corticosteroids and adrenaline, and effects of parathyroid hormone on bone are also elaborated. This volume is recommended for biologists and specialists interested in the culture of cells and tissues.
