Author: Kelly Komachi
Publisher:
ISBN:
Category : Genetic expression
Languages : en
Pages : 404

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Author: Sarah C. R. Elgin
Publisher: Frontiers in Molecular Biology
ISBN: 9780199638901
Category : Chromatin
Languages : en
Pages : 372

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Book Description
Since publication of the first edition in 1995, there have been significant advances and understanding of chromatin structure and its relation to gene expression. These include a high-resolution structure of the nucleosome core, discovery of the enzymes and complexes that mediate histone acetylation and deacetylation, discovery of novel ATP-dependent chromatin remodeling complexes, new insights into nuclear organization and epigenetic silencing mechanisms. In light of these advances, Chromatin Structure and Gene Expression (2ed.) includes updated chapters and additional material that introduce new concepts in the process of gene regulation in chromatin.

Author: Sarah R. Green
Publisher:
ISBN:
Category :
Languages : en
Pages : 308

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Book Description
Eukaryotic transcription is a highly regulated cellular process that represents the balance of positive and negative factors acting on the promoter of a given gene. In Saccharomyces cerevisiae , the Tup1-Sn6 repressor complex negatively influences the expression of approximately five percent of all genes. Functional homologs of this complex exist in other organisms, and a better understanding of the functions of Tup1 in yeast will provide us with insight into the broader questions of how transcriptional repression is regulated across species and the consequences of its misregulation. Most of the targets of Tup1-mediated repression have been identified, but the means by which Tup1 inhibits transcription of these target genes is not entirely clear. To explore this question, we focused on two proteins know to be involved in Tup1-mediated repression--Hda1, a histone deacetylase, and Srb10, a cyclin-dependent kinase associated with the Mediator complex. We disrupted each of these genes separately and in combination and compared the effects of the disruptions on Tup1-regulated genes using a statistical analysis of microarray data. We saw a strong overlap between the genes derepressed in an hda1DELTA strain and the set of Tup1-regulated genes and a smaller but still significant intersection between the mutant srb10 and tup1DELTA datasets. Tup1-regulated genes can be divided into subclasses based on their requirements for Hda1 and/or Srb10 function for full repression. However, the magnitudes of the derepression defect in these mechanistic disruptions are rarely as severe as that of a tup1DELTA strain. We also showed that there was not a strict correlation between the loss of Hda1 deacetylation function and a loss transcriptional repression. These data imply that there are multiple and overlapping mechanisms that contribute to full Tup1-mediated repression and that often several of these mechanisms are acting at a given promoter to repress transcription. We also tried to better understand the mechanisms of Tup1-mediated repression by analyzing the functions of Tup1 itself. We mutagenized a surface of Tup1 conserved among metazoan homologs and measured the effects of the mutants on Tup1-mediated repression by microarray analysis. The mutant alleles represented a range of deficiencies in repression, with the strongest mutant affecting about half of Tup1-regulated genes. For one set of Tup1-regulated genes, some of the point mutants disrupted the recruitment of Tup1 to regulated promoters; however, for the majority of Tup1-repressed genes, the mutant proteins are properly recruited but cannot repress transcription. These point mutants of Tup1 demonstrate that the conserved surface of Tup1 is important for two different aspects of Tup1-mediated repression--recruitment to repressed promoters and the active repression of transcription.

Author: José Ramos
Publisher: Springer
ISBN: 3319253042
Category : Science
Languages : en
Pages : 381

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Book Description
This contributed volume reviews the recent progress in our understanding of membrane transport in yeast including both Saccharomyces cerevisiae and non-conventional yeasts. The articles provide a summary of the key transport processes and put these in a systems biology context of cellular regulation, signal reception and homeostasis. After a general introduction, readers will find review articles covering the mechanisms and regulation of transport for various substrates ranging from diverse nutrients to cations, water and protons. These articles are complemented by a chapter on extremophilic yeast, a chapter on the mathematical modelling of ion transport and two chapters on the role of transport in pathogenic yeasts and antifungal drug resistance. Each article provides both a general overview of the main transport characteristics of a specific substrate or group of substrates and the unique details that only an expert working in the field is able to transmit to the reader. Researchers and students of the topic will find this book to be a useful resource for membrane transport in yeast collecting information in one complete volume, which is otherwise scattered across many papers. This might also be interesting for scientists investigating other species in order to compare transport mechanisms with known functions in yeast with the cells on which they work.

Author: Horst Feldmann
Publisher: John Wiley & Sons
ISBN: 3527644865
Category : Science
Languages : en
Pages : 349

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Book Description
Yeast is one of the oldest domesticated organisms and has both industrial and domestic applications. In addition, it is very widely used as a eukaryotic model organism in biological research and has offered valuable knowledge of genetics and basic cellular processes. In fact, studies in yeast have offered insight in mechanisms underlying ageing and diseases such as Alzheimers, Parkinsons and cancer. Yeast is also widely used in the lab as a tool for many technologies such as two-hybrid analysis, high throughput protein purification and localization and gene expression profiling. The broad range of uses and applications of this organism undoubtedly shows that it is invalubale in research, technology and industry. Written by one of the world's experts in yeast, this book offers insight in yeast biology and its use in studying cellular mechanisms.

Author:
Publisher:
ISBN:
Category : Cells
Languages : en
Pages : 504

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Author: Rebecca Laureen Smith
Publisher:
ISBN:
Category :
Languages : en
Pages : 360

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Author: Christine Guthrie
Publisher:
ISBN: 9780123106704
Category : Molecular biology
Languages : en
Pages : 933

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Book Description
Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Basic Methods in Yeast Genetics**Physical and genetic mapping**Making and recovering mutants**Cloning and Recombinant DNA Methods**High-efficiency transformation**Preparation of yeast artificial chromosome vectors**Basic Methods of Cell Biology**Immunomicroscopy**Protein targeting assays**Biochemistry of Gene Expression**Vectors for regulated expression**Isolation of labeled and unlabeled DNA, RNA, and protein

Author: Michael Redd
Publisher:
ISBN:
Category : Genetic regulation
Languages : en
Pages : 230

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Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1844

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