The Initiation of DNA Replication in Escherichia Coli K-12 PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download The Initiation of DNA Replication in Escherichia Coli K-12 PDF full book. Access full book title The Initiation of DNA Replication in Escherichia Coli K-12 by Nancy Ann Schaus. Download full books in PDF and EPUB format.
Author: Nancy Ann Schaus
Publisher:
ISBN:
Category : DNA
Languages : en
Pages : 342
Get Book Here
Book Description
Author: Nancy Ann Schaus
Publisher:
ISBN:
Category : DNA
Languages : en
Pages : 342
Get Book Here
Book Description
Author: Dan S Ray
Publisher: Elsevier
ISBN: 0323139000
Category : Science
Languages : en
Pages : 649
Get Book Here
Book Description
The Initiation of DNA Replication contains the proceedings of the 1981 ICN-UCLA Symposia on Structure and DNA-Protein Interactions of Replication Origins, held in Salt Lake City, Utah on March 8-13, 1981. The papers explore the initiation of DNA replication and address relevant topics such as whether there are specific protein recognition sites within an origin; how many proteins interact at an origin and whether they interact in a specific temporal sequence; or whether origins can be subdivided into distinct functional domains. The specific biochemical steps in DNA chain initiation and how they are catalyzed are also discussed. This book is organized into six sections and comprised of 41 chapters. The discussion begins by analyzing the replication origin region of the Escherichia coli chromosome and the precise location of the region carrying autonomous replicating function. A genetic map of the replication and incompatibility regions of the resistance plasmids R100 and R1 is described, and several gene products produced in vivo or in vitro from the replication region are considered. The sections that follow focus on the DNA initiation determinants of bacteriophage M13 and of chimeric derivatives carrying foreign replication determinants; suppressor loci in E. coli; and enzymes and proteins involved in initiation of phage and bacterial chromosomes. The final chapters examine the origins of eukaryotic replication. This book will be of interest to scientists, students, and researchers in fields ranging from microbiology and molecular biology to biochemistry, molecular genetics, and physiology.
Author: Michael Dalbey
Publisher:
ISBN:
Category : DNA.
Languages : en
Pages : 378
Get Book Here
Book Description
Author: Anne Eliane Chiaramello
Publisher:
ISBN:
Category : DNA
Languages : en
Pages : 300
Get Book Here
Book Description
The initiation of DNA synthesis at the chromosomal replication origin, oriC, in Escherichia coli involves an RNA polymerase-mediated step. The level of synthesis of transcripts moving counterclockwise toward oriC is controlled at two promoters, P1 (asnC) and P2 (mioC), and at two transcription terminator regions, T1 and T2. As shown by S1 mapping, termination at the T2 region occurs to the right of oriC at nucleotides 297-299 and 306-310, while major termination events in the T1 region occur in and near the mioC promoter. The majority of transcripts entering oriC originates from the mioC promoter. Transcription from the mioC promoter has been shown to enhance the frequency of initiation of DNA replication of oriC containing plasmids, and to stabilize these plasmids in the host cells. The mioC promoter, which is stringently controlled, is also growth rate regulated. The amount of mioC transcripts relative to the amount of total RNA was inversely correlated with growth rate. This transcript is characterized by a short half-life (1.5 min). The mioC promoter, which contains a DnaA protein binding site, was much less susceptible to repression by DnaA protein when located in the chromosome, than when located in a plasmid. Only a very high concentration of DnaA protein repressed the mioC promoter. The DnaA protein, which is required for initiation of DNA replication from oriC, is growth rate regulated. As shown by RNase protection, this regulation is exerted at the transcriptional level, affecting both promoters, dnaAp1 and dnaAp2. Transcription from these two promoters is also stringently controlled. The amount of DnaA protein in spoT mutants, which are deficient in ppGpp pyrophosphorylase activity, decreases as the severity in the mutation increases. Thus, the intracellular concentration of ppGpp influences the expression of the dnaA gene. In conclusion, the growth rate regulation and stringent control of the dnaA gene suggest that one way in which DNA replication is coordinated with the growth rate is via ppGpp synthesis at the ribosome.
Author: Thomas David Daws
Publisher:
ISBN:
Category :
Languages : en
Pages : 272
Get Book Here
Book Description
Author: C. W. Lycett
Publisher:
ISBN:
Category :
Languages : en
Pages :
Get Book Here
Book Description
Author: Gretchen Herpel Stein
Publisher:
ISBN:
Category : DNA.
Languages : en
Pages : 240
Get Book Here
Book Description
Author: Hisao Masai
Publisher: Springer
ISBN: 9811069557
Category : Science
Languages : en
Pages : 581
Get Book Here
Book Description
This book reviews the latest trends and future directions of DNA replication research. The contents reflect upon the principles that have been established through the genetic and enzymatic studies of bacterial, viral, and cellular replication during the past decades. The book begins with a historical overview of the studies on eukaryotic DNA replication by Professor Thomas Kelly, a pioneer of the field. The following chapters include genome-wide studies of replication origins and initiation factor binding, as well as the timing of DNA replications, mechanisms of initiation, DNA chain elongation and termination of DNA replication, the structural basis of functions of protein complexes responsible for execution of DNA replication, cell cycle-dependent regulation of DNA replication, the nature of replication stress and cells’ strategy to deal with the stress, and finally how all these phenomena are interconnected to genome instability and development of various diseases. By reviewing the existing concepts ranging from the old principles to the newest ideas, the book gives readers an opportunity to learn how the classical replication principles are now being modified and new concepts are being generated to explain how genome DNA replication is achieved with such high adaptability and plasticity. With the development of new methods including cryoelectron microscopy analyses of huge protein complexes, single molecular analyses of initiation and elongation of DNA replication, and total reconstitution of eukaryotic DNA replication with purified factors, the field is enjoying one of its most exciting moments, and this highly timely book conveys that excitement to all interested readers.
Author: Raymond G. Fenwick
Publisher:
ISBN:
Category :
Languages : en
Pages : 126
Get Book Here
Book Description
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
Get Book Here
Book Description
