Author: Elena Jimenez-XarriePublisher:
ISBN:
Category :
Languages : en
Pages :
Book Description
Background and Aims:Cardioembolic and large-artery atherothrombosis (LAAT) ischemic stroke are two of the most frequent ischemic stroke etiologies. In some patients, it is not straightforward to find the stroke cause and it remains undetermined. In this context, biomarkers would be a useful diagnostic tool. MicroRNAs (miRNA) have demonstrated potential as biomarkers as they can be detected in blood, are stable, and change dynamically with pathologic processes. The aim of this study was to identify a distinctive miRNA expression profile between cardioembolic and LAAT stroke patients.Methods:A non-targeted screening analysis was performed analyzing 179 miRNAs in plasma from 3 groups: cardioembolic patients (n=8), LAAT patients (n=8) and healthy controls (n=8) with Serum/Plasma Focus microRNA Panel (Exiqon). Afterwards, with the selected miRNAs in the screening phase, a validation analysis was performed with 17 patients in each group and 8 in control group. The 2-u0394Ct relative expression levels were calculated. MiR-29a-3p was used as endogenous control for normalization. Non-parametric Kruskall-Wallis test was used for statistical analysis.Results:In the screening phase, 7 candidate miRNAs (miR-378a-3p, miR-421, miR-127-3p, miR-136-3p, miR-28-3p, miR-629-5p and miR-7-5p) with p
