Synthesis and characterization of silver nanoparticles by "Rhizopus stolonifer" and its activity against multidrug resistant "Escherichia coli" and "Staphylococcus aureus" PDF Download
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Author: Afreen Banu
Publisher: GRIN Verlag
ISBN: 3656211299
Category : Science
Languages : en
Pages : 24
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Book Description
Research Paper (undergraduate) from the year 2011 in the subject Chemistry - Other, Gulbarga University (Microbiology Dept), course: Ph.D, language: English, abstract: This study reports the extracellular synthesis of silver nanoparticles by Rhizopus stolonifer and its efficacy against multidrug resistant (MDR) E.coli and S.aureus isolated from Khwaja Bande Nawas Hospital, Gulbarga, Karnataka. Synthesis of silver nanoparticles (AgNPs) was carried out by using fungal filtrate of R.stolonifer and an aqueous solution of AgNO3. The characterization of AgNPs was made by UV-Visible absorption Spectroscope, Scanning Electron Microscope and Energy Dispersed Spectroscope (SEM-EDS), Transmission Electron Microscope (TEM), Fourier Transform Infrared (FTIR) spectroscopy and Atomic Force Microscope (AFM). TEM micrograph revealed the formation of spherical nanoparticles with size ranging between 3 to 20 nm. Atomic force microscopy gives the three dimensional structure of the particles. The presence of proteins was detected by FTIR spectroscopy. Three dimensional structure of AgNPs was studied by AFM. AgNPs produced by R.stolonifer gave good antibacterial activity against clinical isolates which were multidrug resistant. Here we report the efficacy of mycogenic metal nanosilver against MDR strains which is difficult through conventional chemotherapy.
Author: Afreen Banu
Publisher: GRIN Verlag
ISBN: 3656211299
Category : Science
Languages : en
Pages : 24
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Book Description
Research Paper (undergraduate) from the year 2011 in the subject Chemistry - Other, Gulbarga University (Microbiology Dept), course: Ph.D, language: English, abstract: This study reports the extracellular synthesis of silver nanoparticles by Rhizopus stolonifer and its efficacy against multidrug resistant (MDR) E.coli and S.aureus isolated from Khwaja Bande Nawas Hospital, Gulbarga, Karnataka. Synthesis of silver nanoparticles (AgNPs) was carried out by using fungal filtrate of R.stolonifer and an aqueous solution of AgNO3. The characterization of AgNPs was made by UV-Visible absorption Spectroscope, Scanning Electron Microscope and Energy Dispersed Spectroscope (SEM-EDS), Transmission Electron Microscope (TEM), Fourier Transform Infrared (FTIR) spectroscopy and Atomic Force Microscope (AFM). TEM micrograph revealed the formation of spherical nanoparticles with size ranging between 3 to 20 nm. Atomic force microscopy gives the three dimensional structure of the particles. The presence of proteins was detected by FTIR spectroscopy. Three dimensional structure of AgNPs was studied by AFM. AgNPs produced by R.stolonifer gave good antibacterial activity against clinical isolates which were multidrug resistant. Here we report the efficacy of mycogenic metal nanosilver against MDR strains which is difficult through conventional chemotherapy.
Author: Afreen Banu
Publisher:
ISBN: 9783656211815
Category :
Languages : en
Pages : 28
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Book Description
Scholarly Research Paper from the year 2011 in the subject Chemistry - Other, grade: -, Gulbarga University (Microbiology Dept), course: Ph.D, language: English, abstract: This study reports the extracellular synthesis of silver nanoparticles by Rhizopus stolonifer and its efficacy against multidrug resistant (MDR) E.coli and S.aureus isolated from Khwaja Bande Nawas Hospital, Gulbarga, Karnataka. Synthesis of silver nanoparticles (AgNPs) was carried out by using fungal filtrate of R.stolonifer and an aqueous solution of AgNO3. The characterization of AgNPs was made by UV-Visible absorption Spectroscope, Scanning Electron Microscope and Energy Dispersed Spectroscope (SEM-EDS), Transmission Electron Microscope (TEM), Fourier Transform Infrared (FTIR) spectroscopy and Atomic Force Microscope (AFM). TEM micrograph revealed the formation of spherical nanoparticles with size ranging between 3 to 20 nm. Atomic force microscopy gives the three dimensional structure of the particles. The presence of proteins was detected by FTIR spectroscopy. Three dimensional structure of AgNPs was studied by AFM. AgNPs produced by R.stolonifer gave good antibacterial activity against clinical isolates which were multidrug resistant. Here we report the efficacy of mycogenic metal nanosilver against MDR strains which is difficult through conventional chemotherapy.
Author: Rathi C.R
Publisher: Prem Jose
ISBN:
Category : Education
Languages : en
Pages :
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Book Description
Mirabilis jalapa is one of the medicinally important shrubs used in traditional medicine, belongs to the family Nyctaginaceae and commonly called as 4’O clock plant. M. jalapa plant extract contains biologically active substances that exhibit microbicidal or microstatic effects against various diseases causing microbes. The plant has high medicinal properties such that the indigenous people of Mexico use it to cure many infirmities including dysentery, diarrhoea, muscular pain and abdominal colic. It is used for the treatment of various infections. Parts of the plant are used to combat dyspepsia and other digestive disorders. The extract of yellow, red color flower variety and green leaf of M. jalapa can be used as natural food color instead of artificial color. The main advantage of using these plant extracts are they have high antimicrobial and have high antioxidant activity. It has the capacity to resist the bacteria like Bacillus, Salmonella, Klebsiella, E coli, Staphylococcus with the help of Silver nanoparticles. The plant has also proved to possess antibacterial, antiviral, and antioxidant activity. Nanoparticles are defined as particulate dispersions or solid particles with a size in the range of 10-1000nm. The drug is dissolved, entrapped, encapsulated or attached to a nanoparticle matrix. Depending upon the method of preparation, nanoparticles, nanospheres or nanocapsules can be obtained. Nanocapsules are systems in which the drug is confined to a cavity surrounded by a unique polymer membrane, while nanospheres are matrix systems in which the drug is physically and uniformly dispersed. Antioxidants are used as food additives to help guard against food deterioration. Exposure to oxygen and sunlight are the two main factors in the oxidation of food, so food is preserved by keeping in the dark and sealing it in containers or even coating it in wax, as with cucumbers. However, as oxygen is also important for plant respiration, storing plant materials in anaerobic conditions produces unpleasant flavours and unappealing colors. Consequently, packaging of fresh fruits and vegetables contains an ~8% oxygen atmosphere.
Author: Prashant Kesharwani
Publisher: Elsevier
ISBN: 0443153442
Category : Medical
Languages : en
Pages : 501
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Book Description
Silver Nanoparticles for Drug Delivery is a complete reference that provides comprehensive coverage of the principle behind the formation, characterization, application, regulation, toxicity, and challenges and future perspectives of silver nanoparticles in drug delivery for the treatment of various diseases. Sections cover the synthesis and characterization of silver nanoparticles (AgNPs), historical introductions to developments, methods and characterization parameters, green and biological synthesis methods, biofate and cellular interactions of AgNPs, drug delivery for the treatment of several diseases and conditions, targeting strategies, applications in cancer diagnosis and treatment, and much more. This is a complete reference for graduate students in nanotechnology drug delivery. Delivers the complete journey of silver nanoparticles in drug delivery, from the fundamentals to the most recent applications Provides an in-depth novel application of silver nanoparticles in biological milieu Presents a vital source for researchers in silver nanoparticles based targeted drug delivery, nanomedicine and engineering
Author: Humaira Khatoon
Publisher: Anchor Academic Publishing
ISBN: 3960670885
Category : Science
Languages : en
Pages : 49
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Book Description
In modern days, Nanotechnology is in wide use in various domains of science. It deals with the Nanoparticles having a size of 1-100 nm in one dimension. It is often used in medical chemistry, atomic physics and other scientific disciplines. The synthesis of nanomaterials is of current interest due to their wide variety of applications in fields such as electronics, photonics, catalysis, medicine etc. The applications of nanotechnology are growing because matter at the nanometer scale has different properties compared with the bulk state. For this reason, many research groups around the world are testing new methods of synthesis of different materials at the nanoscale. Silver nanoparticles (AgNPs) have been the subject matter of researchers due to their unique properties (e.g. size and shape depending on optical, antimicrobial and electrical properties).
Author: G. Ali Mansoori
Publisher: World Scientific
ISBN: 9812561544
Category : Technology & Engineering
Languages : en
Pages : 358
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Book Description
- Gives unified presentation of many of the major principles in nanotechnology: molecular-based study of condensed matter in small systems. - Authored by an expert in the molecular-based study of matter - Accessible to students, yet of interest to experts - Emphasizes the intrinsic beauty of methods of bottom-up nanotechnology - Includes many full-color figures
Author: Kamel A. Abd-Elsalam
Publisher: Elsevier
ISBN: 0128245093
Category : Technology & Engineering
Languages : en
Pages : 798
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Book Description
Green Synthesis of Silver Nanomaterials illustrates how to biologically scale up silver nanoparticle synthesis. This book covers green synthesis of silver nanomaterials, via plants, agricultural waste, fungi, and microorganisms. Sections cover the synthesis and characterization of chemical and green synthesis, various types of silver nanomaterialism, the ability of different fungal species, such as filamentous fungi, to produce silver nanoparticles, the microbial synthesis of silver NMs, biosynthesis mechanisms, toxicity, fate and commercialization. As examples, greener pathways and mechanisms, toxicity of silver nanoparticles in aquatic life and in natural eco-systems, and strategies for the scaling up of green-synthesized nanomaterials are discussed. With the extended work in enhancing nanomaterials synthesis performance, and discovering their biomedical, environmental, and agricultural applications, it is hoped that the execution of these methods on a large scale and their industrial applications in different fields will take place in the near future. - Assesses the impact of a large variety of silver-based nanostructures in the biomedical, environmental and agri-food sectors - Discusses the major synthesis methods used for effectively processing plant-based silver nanoparticles - Outlines the potential and major challenges for adopting green synthesis methods on a mass scale
Author: Ram Prasad
Publisher: Springer
ISBN: 3319684248
Category : Science
Languages : en
Pages : 299
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Book Description
Fungal nanotechnology has great prospects for developing new products with industrial, agricultural, medicinal, and consumer applications in a wide range of sectors. The fields of chemical engineering, agri-food, biochemistry, pharmaceuticals, diagnostics, and medical device development all employ fungal products, with fungal nanomaterials currently used in applications ranging from drug development to the food industry and agricultural biotechnology. Fungal agents are an environmentally friendly, clean, non‐toxic agent for the synthesis of metal nanoparticles and employ both intracellular and extracellular methods. The simplicity of scaling up and downstream processing and the presence of fungal mycelia which afford an increased surface area provide key advantages. In addition, the large spectrum of synthesized nanoparticle morphologies and the substantially faster biosynthesis rate in cell-free filtrate (due to the higher amount of proteins secreted in fungi) make this a particularly enticing route. Understanding the diversity of fungi in assorted ecosystems, as well as their interactions with other microorganisms, animals, and plants, underpins real and innovative technological developments and the applications of metal nanoparticles in many disciplines including agriculture, catalysis, and biomedical biosensors. Importantly, biogenic fungal nanoparticles show significant synergistic characteristics when combined with antibiotics and fungicides to offer substantially greater resistance to microbial growth and applications in nanomedicine ranging from topical ointments and bandages for wound healing to coated stents.
Author: Simasiku Sinvula
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
It is of great concern that bacteria are forming resistance to common antibiotics meant to prevent or cure them. For this reason, researchers are engaged in synthesis of nanoparticles using medicinal plants to solve the problem of antibiotic resistance in bacteria. The aim of the study was to synthesize silver nanoparticles (AgNPs) using M. oleifera extracts and compare antibacterial activities of AgNPs prepared in M. oleifera extracts with AgNPs of common antibiotics (penicillin G and streptomycin) against antibiotic resistant strains of Escherichia coli and Staphylococcus aureus. Synthesized AgNPs were characterized using UV-vis spectroscopy, fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Disc diffusion method was used for initial screening of antibacterial activities and their minimum inhibitory concentration (MIC) was determined. Formation of AgNPs was confirmed by colour change from pale yellow to dark brown-black. UV-vis spectra analysis showed maximum peak of 400-440 nm. FTIR analysis revealed the presence of hydroxyl and carbonyl functional groups, as well as C-N and C-O bonds that might be responsible for capping, stabilizing and reducing AgNPs. SEM analysis revealed crystalline AgNPs with mean average size of 46.49 ± 0.73 nm to 60.96 ± 1.17 nm. The combination of AgNPs with antibiotics showed better antibacterial activities as compared to AgNPs alone and streptomycin alone. Lowest MIC values were 12.5 mg/mL, 1.56 mg/mL and 3.13 mg/mL for Mo-AgNPs, Mo-AgNPs-Penicillin G and Mo-AgNPs-streptomycin combination respectively. It can then be concluded that M. oleifera has strong potential for synthesis of AgNPs, and combination of AgNPs with common antibiotics sheds light in the direction of decreasing odds of antibiotic resistance development of these pathogens.
Author: Daniel L. Fedlheim
Publisher: CRC Press
ISBN: 9780585404394
Category : Science
Languages : en
Pages : 348
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Book Description
A state-of-the-art reference, Metal Nanoparticles offers the latest research on the synthesis, characterization, and applications of nanoparticles. Following an introduction of structural, optical, electronic, and electrochemical properties of nanoparticles, the book elaborates on nanoclusters, hyper-Raleigh scattering, nanoarrays, and several applications including single electron devices, chemical sensors, biomolecule sensors, and DNA detection. The text emphasizes how size, shape, and surface chemistry affect particle performance throughout. Topics include synthesis and formation of nanoclusters, nanosphere lithography, modeling of nanoparticle optical properties, and biomolecule sensors.
