Synthesis and Characterization of Methacrylated Hyaluronan-based Hydrogels for Tissue Engineering PDF Download
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Author: Keegan Bradley Compton
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Keegan Bradley Compton
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Pietro Matricardi
Publisher: CRC Press
ISBN: 9814613622
Category : Medical
Languages : en
Pages : 527
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Book Description
Hydrogels are an emerging area of interest in medicine as well as pharmaceutics, and their physico-chemical characterization is fundamental to their practical applications. Compared with synthetic polymers, polysaccharides that are widely present in living organisms and come from renewable sources are extremely advantageous for hydrogel formation.
Author: Sougata Jana
Publisher: Springer Nature
ISBN: 9819988268
Category :
Languages : en
Pages : 427
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Author: Michael Jury
Publisher: Linköping University Electronic Press
ISBN: 9179293433
Category :
Languages : en
Pages : 128
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Three-dimensional (3D) cell culture facilitates development of biological relevant assays for drug screening and toxicity testing. Compared to conventional 2D cell culture, cells cultured in 3D can more accurately mimic human tissues and organs and thus provide ex vivo data with potentially better predictive value for cancer research, pharmacology, and toxicology, reducing the need for animal models, improving experimental reproducibility, and reducing time and costs in drug development. The most widely used options for scaffold-based 3D cell culture are, however, based on poorly defined biologically derived extracellular matrix (ECM) with limited possibilities to tailor material properties and that are difficult to combine with state-of-the art biofabrication techniques. The overall aim this thesis was to design and explore modular hyaluronan (HA) based ECM-mimicking hydrogels with tuneable physiochemical properties and biofunctionalities, for development of advanced 3D cell models and biofabrication. The thesis work is presented in five papers. In paper I, we used copper free click chemistry for both hydrogel cross-linking and functionalization with fibronectin derived peptide sequences for culture of human induced pluripotent-derived hepatocytes in a perfused microfluidic system. The tuneable and bioorthogonal cross-linking enabled both retention of high cell viabilities and fabrication of a functional liver-on-chip solution. In paper II, we combined the developed HA-based hydrogel system with homo- and heterodimerizing helix-loop-helix peptides for modulation of both cross-linking density and biofunctionalization. We further demonstrated the possibilities to use these hydrogels as bioinks for 3D bioprinting where both the molecular composition and the physical properties of the printed structures could be dynamically altered, providing new avenues for four-dimensional (4D) bioprinting. In paper III we investigated the possibilities to chemically conjugate full size recombinant human laminin-521 (LN521) in the HA-based hydrogels system using copper-free click chemistry, with the aim to enable 3D culture and 3D bioprinting of neurons. We quantified the impact of using different linkers to tether LN521 and the influence of LN-functionalization on the structural and mechanical properties of the hydrogels. We show that both differentiated and non-differentiated neuroblastoma cells and long-term self-renewing neuroepithelial stem cells (lt-NES) remained viable in the hydrogels. The hydrogels also had a protected effect on lt-NES during syringe ejection and bioprinting. In paper IV, we used HA-based hydrogels modified with peptides sequences derived from fibronectin and laminin for culture of fetal primary astrocytes (FPA). We explored both the interactions between the hydrogels and FPA and possibilities to 3D bioprint FPAs. Finally, in paper V, we developed HA-nanocellulose composite hydrogels with the aim to increase printing fidelity and enable fabrication of multi-layered bioprinted structures without the use of a support bath. In addition to HA, we used wood-fibre derived nanocellulose (NC) to increase the viscosity of the bioink during the printing process. The developed biorthogonal and modular hydrogel systems provide a large degree of flexibility that allows for encapsulation and culture of different cell types and processing using different techniques, which can contribute to further exploration of fabrication of biologically relevant tissue and disease models.
Author: Thakur Raghu Raj Singh
Publisher: CRC Press
ISBN: 1498748627
Category : Medical
Languages : en
Pages : 353
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Book Description
Hydrogels are crosslinked, macromolecular polymeric materials arranged in a three-dimensional network, which can absorb and retain large amounts of water. Hydrogels are commonly used in clinical practice and experimental medicine for a wide range of applications, including drug delivery, tissue engineering and regenerative medicine, diagnostics, cellular immobilization, separation of biomolecules or cells, and barrier materials to regulate biological adhesions. This book elucidates the underlying concepts and emerging applications of hydrogels and will provide key case studies and critical analysis of the existing research.
Author: Kimberly A. McEwan
Publisher:
ISBN:
Category : University of Ottawa theses
Languages : en
Pages :
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The expanding field of tissue engineering provides a new approach to regenerative medicine for common ailments such as cardiovascular disease and type-I diabetes. Biomaterials can be administered as a delivery vehicle to introduce therapeutic cells to sites of damaged or diseased tissue. A specific class of biomaterials, termed hydrogels, is suitable for this application as they can provide a biocompatible, biodegradable scaffold that mimics the physical properties of the native soft tissue. Injectable hydrogels are increasingly being developed for biomedical applications due to their ability to be delivered in a minimally invasive manner. One potential use for such materials is in the delivery of therapeutics such as cells or growth factor-releasing particles. In this study, the first aim was to determine the interactive effects between collagen-based hydrogels and additives (cells and microspheres) for cardiac regeneration. The results demonstrated that the addition of either cells or microspheres to a collagen-based hydrogel decreased its gelation time and increased its viscosity. Increased cross-linker concentrations resulted in lower cell viability. However, this cell loss could be minimized by delivering cells with the cross-linker neutralizing agent, glycine. As a potential application of these materials, the second aim of this study was to develop a hydrogel for use as an ectopic islet transplant site. Specifically, collagen-chitosan hydrogels were synthesized and characterized, with and without laminin, and tested for their ability to support angiogenic and islet cell survival and function. Matrices synthesized with lower chitosan content (20:1 collagen:chitosan) displayed greater cell compatibility for both angiogenic cells and for islets and weaker mechanical properties, while matrices with higher chitosan content (10:1 collagen:chitosan) had the opposite effect. Laminin did not affect the physical properties of the matrices, but did improve angiogenic cell and islet survival and function. Overall the proposed collagen-based hydrogels can be tailored to meet the physical property requirements for cardiac and islet tissue engineering applications and demonstrated promising cell support capabilities.
Author: Shakeel Ahmed
Publisher: Elsevier
ISBN: 0323993427
Category : Technology & Engineering
Languages : en
Pages : 675
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Book Description
Polysaccharide-Based Hydrogels: Synthesis, Characterization and Applications looks at the synthesis, characterization and application of polysaccharide-based materials in a broad array of fields. The book discusses the role of polysaccharides in the preparation of hydrogels, the use of hydrogel-based green materials, and their applications in biomedical applications, drug delivery, water purification techniques, food industries, agricultural fields, and pharmaceuticals applications. Written by leading experts in this field, this book will be a valuable reference for scientists, academicians, researchers, technologists, consultants and policymakers. - Explains origin, extraction, processing, structural analysis and applications of polysaccharides-based hydrogels - Includes chapters that specifically focus on a particular hydrogel - Provides specific applications of polysaccharide hydrogels
Author: Kyriacos A. Athanasiou
Publisher: Morgan & Claypool Publishers
ISBN: 1598298755
Category : Medical
Languages : en
Pages : 183
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Book Description
Cartilage injuries in children and adolescents are increasingly observed, with roughly 20% of knee injuries in adolescents requiring surgery. In the US alone, costs of osteoarthritis (OA) are in excess of $65 billion per year (both medical costs and lost wages). Comorbidities are common with OA and are also costly to manage. Articular cartilage's low friction and high capacity to bear load makes it critical in the movement of one bone against another, and its lack of a sustained natural healing response has necessitated a plethora of therapies. Tissue engineering is an emerging technology at the threshold of translation to clinical use. Replacement cartilage can be constructed in the laboratory to recapitulate the functional requirements of native tissues. This book outlines the biomechanical and biochemical characteristics of articular cartilage in both normal and pathological states, through development and aging. It also provides a historical perspective of past and current cartilage treatments and previous tissue engineering efforts. Methods and standards for evaluating the function of engineered tissues are discussed, and current cartilage products are presented with an analysis on the United States Food and Drug Administration regulatory pathways that products must follow to market. This book was written to serve as a reference for researchers seeking to learn about articular cartilage, for undergraduate and graduate level courses, and as a compendium of articular cartilage tissue engineering design criteria. Table of Contents: Hyaline Articular Cartilage / Cartilage Aging and Pathology / In Vitro / Bioreactors / Future Directions
Author: Md. Ibrahim H. Mondal
Publisher: Springer
ISBN: 9783319778297
Category : Technology & Engineering
Languages : en
Pages : 0
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Book Description
With the prospect of revolutionizing specific technologies, this book highlights the most exciting and impactful current research in the fields of cellulose-based superabsorbent hydrogels with their smart applications. The book assembles the newest synthetic routes, characterization methods, and applications in the emergent area. Leading experts in the field have contributed chapters representative of their most recent research results, shedding light on the enormous potential of this field and thoroughly presenting cellulose-based hydrogel functioning materials. The book is intended for the polymer chemists, academic and industrial scientists and engineers, pharmaceutical and biomedical scientists and agricultural engineers engaged in research and development on absorbency, absorbent products and superabsorbent hydrogels. It can also be supportive for undergraduate and graduate students.
Author: Weifeng Zhao
Publisher: Frontiers Media SA
ISBN: 2889638960
Category :
Languages : en
Pages : 130
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Book Description
