Studies on the Roles of DNA Polymerases Delta and Epsilon in DNA Replication and Repair, and the Interaction of the Small Subunit of DNA Polymerase Delta with Proliferating Cell Nuclear Antigen PDF Download
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Author: Min You
Publisher:
ISBN:
Category : DNA polymerases
Languages : en
Pages : 186
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Author: Min You
Publisher:
ISBN:
Category : DNA polymerases
Languages : en
Pages : 186
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Book Description
Author: Giovanni Maga
Publisher: World Scientific
ISBN: 9813226420
Category : Science
Languages : en
Pages : 398
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Book Description
Maintenance of the information embedded in the genomic DNA sequence is essential for life. DNA polymerases play pivotal roles in the complex processes that maintain genetic integrity. Besides their tasks in vivo, DNA polymerases are the workhorses in numerous biotechnology applications such as the polymerase chain reaction (PCR), cDNA cloning, next generation sequencing, nucleic acids based diagnostics and in techniques to analyze ancient and otherwise damaged DNA (e.g. for forensic applications). Moreover, some diseases are related to DNA polymerase defects and chemotherapy through inhibition of DNA polymerases is used to fight HIV, Herpes and Hepatitis B and C infections. This book focuses on (i) biology of DNA polymerases, (ii) medical aspects of DNA polymerases and (iii) biotechnological applications of DNA polymerases. It is intended for a wide audience from basic scientists, to diagnostic laboratories, to companies and to clinicians, who seek a better understanding and the practical use of these fascinating enzymes.
Author: Ulrich Hbscher
Publisher: World Scientific
ISBN: 9814299162
Category : Science
Languages : en
Pages : 338
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Book Description
Maintenance of the information embedded in the genomic DNA sequence is essential for life. DNA polymerases play pivotal roles in the complex physiological processes of DNA replication and repair. Besides the tasks in vivo, DNA polymerases are the workhorses in numerous biotechniques such as polymerase chain reaction (PCR), cDNA cloning, genome sequencing, nucleic acids–based diagnostics, as well as techniques to analyze ancient and otherwise damaged DNA. The authors have recently witnessed the discovery of a plethora of novel DNA polymerases with specialized properties whose physiological functions are only just beginning to be understood. This book summarizes the current knowledge of these fascinating enzymes in viruses, bacteria, archaea and eukaryotes. Moreover, some diseases are related to DNA polymerase defects, and chemotherapy through inhibition of DNA polymerases is used to fight HIV, Herpes, as well as Hepatitis B and C infections. This book will appeal to a broad audience including basic scientists, diagnostic laboratories, and clinicians who will gain an invaluable understanding of these fascinating enzymes.
Author: Joseph L. Stodola
Publisher:
ISBN:
Category : Electronic dissertations
Languages : en
Pages : 178
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Book Description
The work described in this dissertation focuses on several aspects of DNA replication in the model organism Saccharomyces cerevisiae, with particular attention paid to the function of the replicative DNA polymerase [delta] (Pol [delta]), and its functions in Okazaki fragment synthesis and maturation. The first major theme of this dissertation is investigating the role that metal binding motifs play in the structure and function of Pol [delta] and other budding yeast polymerases. First, I discuss the role that two metal binding motifs within the catalytic subunit of Pol [delta] play in creating the multi-subunit polymerase complex and in promoting crucial interactions with the replication sliding clamp, proliferating cell nuclear antigen (PCNA). Next, I describe work defining the importance of similar metal binding motifs in the translesion DNA polymerase [zeta] (Pol [zeta]). This yielded the observation that the two accessory subunits of Pol [delta], Pol31 and Pol32, are also constitutive members of a four-subunit Pol [zeta] complex. Finally, I describe the creation of a chimeric DNA polymerase comprising the bacteriophage RB69 DNA polymerase fused to the metal binding domain of the Pol [delta] catalytic subunit. We show that this chimeric polymerase can form a multimeric complex containing the Pol [delta] accessory subunits, interact with PCNA, and support DNA replication in vivo. This data provided insight into the structural requirements of the lagging strand replication machinery. The second major theme is Pol [delta]'s crucial role in synthesizing Okazaki fragments and participating in the removal of initiator RNA, called Okazaki fragment maturation. I first describe my work developing a system to study the activity of Pol [delta] in higher kinetic detail than previous studies, using rapid-quench techniques. This work yielded insights into how Pol [delta] performs DNA synthesis and strand displacement synthesis, as well as accomplishes nick translation, requiring collaboration between Pol [delta] and the flap-endonuclease FEN1. The next chapter describes the production and characterization of engineered PCNA heterotrimers. These proteins were produced to test the 'toolbelt' model, which is the hypothesis that PCNA binds multiple enzymes simultaneously to increase the efficiency of DNA metabolism processes involving multiple enzymes. Finally, there has been a growing interest among those studying lagging strand synthesis into how potential impediments to the Okazaki fragment maturation machinery are resolved; we show that although the transcription factor Rap1 can block strand displacement synthesis by Pol [delta] when it is bound to DNA, this block can be resolved through the action of the helicase Pif1. In sum, these studies provide insight into how Pol [delta]'s structure dictates its function, as well as addresses larger mechanistic questions concerning how lagging strand DNA replication is accomplished.
![Roles of DNA Polymerase [delta] and PCNA in DNA Replication and Repair PDF](https://books.google.com/books/content/images/frontcover/cbDcNwAACAAJ?fife=w80-h100)
Author: Parie Garg
Publisher:
ISBN:
Category :
Languages : en
Pages : 242
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Book Description
Author: David C. Amberg
Publisher: CSHL Press
ISBN: 0879697288
Category : Genetics
Languages : en
Pages : 250
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Book Description
"Methods in Yeast Genetics" is a course that has been offered annually at Cold Spring Harbor for the last 30 years. This provides a set of teaching experiments along with the protocols and recipes for the standard techniques and reagents used in the study of yeast biology.
Author: Zvi Kelman
Publisher: Frontiers Media SA
ISBN: 2889194558
Category : Biotechnology
Languages : en
Pages : 147
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Book Description
DNA polymerases are core tools for molecular biology including PCR, whole genome amplification, DNA sequencing and genotyping. Research has focused on discovery of novel DNA polymerases, characterization of DNA polymerase biochemistry and development of new replication assays. These studies have accelerated DNA polymerase engineering for biotechnology. For example, DNA polymerases have been engineered for increased speed and fidelity in PCR while lowering amplification sequence bias. Inhibitor resistant DNA polymerase variants enable PCR directly from tissue (i.e. blood). Design of DNA polymerases that efficiently incorporate modified nucleotide have been critical for development of next generation DNA sequencing, synthetic biology and other labeling and detection technologies. The Frontiers in Microbiology Research Topic on DNA polymerases in Biotechnology aims to capture current research on DNA polymerases and their use in emerging technologies.
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1828
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Author: Xiu-Qing Li
Publisher: John Wiley & Sons
ISBN: 1118647122
Category : Science
Languages : en
Pages : 512
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Book Description
Written by an international team of experts, Somatic Genome Variation presents a timely summary of the latest understanding of somatic genome development and variation in plants, animals, and microorganisms. Wide-ranging in coverage, the authors provide an updated view of somatic genomes and genetic theories while also offering interpretations of somatic genome variation. The text provides geneticists, bioinformaticians, biologist, plant scientists, crop scientists, and microbiologists with a valuable overview of this fascinating field of research.
Author: Zachary Forest Pursell
Publisher:
ISBN:
Category :
Languages : en
Pages : 294
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Book Description
