Studies on the Membrane Protein Assembly in Escherichia Coli PDF Download
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Author: Guoqing Cao
Publisher:
ISBN:
Category :
Languages : en
Pages : 220
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Author: Guoqing Cao
Publisher:
ISBN:
Category :
Languages : en
Pages : 220
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Book Description
Author: Gunnar von Heijne
Publisher: R. G. Landes
ISBN:
Category : Science
Languages : en
Pages : 300
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Author: Michael Wilson Laird
Publisher:
ISBN:
Category : Cell membranes
Languages : en
Pages : 554
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Author: Gunnar von Heijne
Publisher: R. G. Landes
ISBN:
Category : Science
Languages : en
Pages : 298
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Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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A genetic system for directly expressing proteins in E. coli and assessing their ability to insert into the bacterial cytoplasmic membrane was developed, consisting of a direct expression vector, and a mature P-lactamase cassette plasmid for generating translational fusions of P-lactamase to the bacterially expressed proteins. This system was used to analyse the fate of three different eukaryotic plasma membrane proteins in E. coli. The ampicillin resistance/sensitivity phenotypes of the P-lactamase fusion proteins revealed that the P-subunit of the sheep-kidney Na,K-ATPase, a class II membrane protein, inserts into the cytoplasmic membrane of E. coli, with the same topology as in its native membrane. The Alzheimer's disease amyloid A4 protein precursor, a class I membrane protein, was also found to insert into the bacterial cytoplasmic membrane, and, although preliminary, the results were compatible with its correct topological insertion into the bacterial cytoplasmic membrane. In contrast, the P2-adrenergic receptor, a complex membrane protein that has an extracellular amino-terminal domain and seven transmembrane segments, was found to misinsert into the E. coli cytoplasmic membrane. A plasmid vector for cloning sequences able to function as cytoplasmic membrane translocators of P-lactamase in E. coli was also developed. Short DNA fragments of essentially random sequence were cloned, and recombinants with functional translocators were directly selected. The spectrum of random sequences able to function as bacterial cytoplasmic membrane translocators suggests that the hydrophobicity requirements for translocation across the bacterial cytoplasmic membrane and yeast endoplasmic reticulum membrane may be more similar than previously assumed.In the above studies new and widely applicable uses of P-lactamase fusion technology have been described. As applied in this thesis they highlight similarities and differences between the mechanisms by which proteins assemble into, or are translocated across, the bacterial cytoplasmic membrane and the eukaryotic endoplasmic reticulum membrane.
Author: Yianbiao Zhang
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Bruce Alberts
Publisher:
ISBN: 9780815332183
Category : Cytology
Languages : en
Pages : 0
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Author: R.E. Dalbey
Publisher: Elsevier
ISBN: 0080526098
Category : Science
Languages : en
Pages : 297
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Book Description
The incentive for putting together Volume 4 of this series was to review the wealth of new information that has become available in prokaryotic organisms in protein export and membrane biogenesis. Just in the last several years, protein translocation has now been efficiently reconstituted using defined components and the mechanism by which proteins are moved across membrane bilayers is now being examined at a higher resolution. In addition, because of a new technical breakthrough using osmolytes, it is now possible to reconstitute a number of channel proteins, ATPase, receptors, and transporters. In many cases, it is possible to successfully predict the membrane topology of these types of proteins using both "hydrophobicity analysis" and the "positive inside" rule. In this volume, two chapters focus on protein translocation across membranes (Biochemical Analyses of Components Comprising the Protein Translocation Machinery of E. Coli; Protein Translocation Genetics), while several others on how proteins assemble into the ineer membrane of E. Coli (Membrane Protein Assembly; Membrane Insertion of Small Proteins: Evolutionary and Functional Aspects; Pigment-Protein Complex Assembly in Rhodobacter sphaeroides and Rhodobacter Capsulatus). Other sections review recent progress on transporters (Identification and Reconstitution of Anion Exchange Mechanisms in Bacteria; Helic Packing in the C-Terminal Half of Lactose Permease) and signal transduction (Mechanism of Transmembrane Signaling in Osmoregulation) as well as the assembly of prints into the outer membrane (Export and Assembly of Outer Membrane Proteins in E. coli). Although the emphasis of the book is on proteins, the role of phospholipids in controlling various cell surface processes is reviewed (Role of Phospholipids in coli Cell Function). I should point out the reason for the rapid progress in bacteria research is because of the possibility to apply biochemistry and genetics in this organism.
Author: Konstantinos Mitsopoulos
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Jos A.F. Op den Kamp
Publisher: Springer Science & Business Media
ISBN: 3642731848
Category : Science
Languages : en
Pages : 474
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Book Description
Many individual aspects of the dynamics and assembly of biological membranes have been studied in great detail. Cell biological approaches, advanced genetics, biophysics and biochemistry have greatly contributed to an increase in our knowledge in this field.lt is obvious however, that the three major membrane constituents - lipids, proteins and carbohydrates- are studied, in most cases separately and that a coherent overview of the various aspects of membrane biogenesis is not readily available. The NATO Advanced Study Institute on "New Perspectives in the Dynamics of Assembly of Biomembranes" intended to provide such an overview: it was set up to teach students and specialists the achievements obtained in the various research areas and to try and integrate the numerous aspects of membrane assembly into a coherent framework. The articles in here reflect this. Statting with detailed contributions on phospholipid structure, dynamics, organization and biogenesis, an up to date overview of the basic, lipidic backbone of biomembranes is given. Extensive progress is made in the research on membrane protein biosynthesis. In particular the post- and co-translational modification processes of proteins, the mechanisms of protein translocation and the sorting mechanisms which are necessary to direct proteins to their final, intra - or extracellular destination have been characterized in detail. Modern genetic approaches were indispensable in this research area: gene cloning, hybrid protein construction, site directed mutagenesis and sequencing techniques elucidated many functional aspects of specific nucleic acid and amino acid sequences.
