Studies on the Development of Instrumental Insemination of Queen Honey Bees (Apis Mellifera L.): Semen Collection, Storage and Their Effects on the Onset of Oviposition PDF Download
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Author: Osman Kaftanoglu
Publisher:
ISBN:
Category : Bees
Languages : en
Pages : 174
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Author: Osman Kaftanoglu
Publisher:
ISBN:
Category : Bees
Languages : en
Pages : 174
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Author: Otto Mackensen
Publisher:
ISBN:
Category : Bee culture
Languages : en
Pages : 32
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Author: Marilène Paillard
Publisher:
ISBN:
Category :
Languages : en
Pages : 79
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Honey bees (Apis mellifera Linnaeus) are critical players in the agricultural industry for food production as they account for the vast majority of insect pollination. In the last decades, however, there have been dramatic losses of honey bee colonies worldwide. Coupled with instrumental insemination, conservation of honey bee sperm is an effective strategy to protect the species and their genetic diversity. Sperm storage is possible at room temperature, but for many mammal species, cryopreservation is the preferred method for the long-term storage of gametes. However, cryopreservation of honey bee drone semen is not optimized. Our overall objective is to develop a method of drone semen cryopreservation, therefore, two experiments were conducted. Hypothesis #1 was that cryopreservation of drone semen is more effective for long-term storage than at above-freezing temperatures. We therefore compared the efficacy based on sperm viability, of two honey bee semen preservation temperatures: frozen (-196°C) and 16°C. After 1 year of storage, frozen sperm viability was higher than at 16°C (76% ± 5% vs. 0%; p 0.05), showing that cryopreservation is necessary to conserve semen in vitro. However, the cryoprotectant used for drone sperm freezing, DMSO (dimethyl sulfoxide), is toxic to queens after instrumental insemination. Hypothesis #2, therefore, was that centrifugation of cryopreserved semen to remove DMSO prior to insemination improves queen fertility. Our results indicate that centrifuging semen does not affect sperm viability (78% ± 3% vs 75% ± 4% viable sperm; p 0.05). After queen insemination, both spermathecae and brood production were evaluated, but the results varied greatly, possibly due to the undesirable mucus present in the semen. Therefore, we cannot yet confirm that centrifugation improves queen health after insemination. Nonetheless, our study confirms that cryopreservation of honey bee sperm is necessary and possible for long-term conservation.
Author: Jan Tyl
Publisher: Výzkumný ústav včelařský, s.r.o.
ISBN: 8087196260
Category :
Languages : en
Pages : 65
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Contents 1. Introduction 2. Breeding drones 3. Breeding of queen bees 4. Assembly and maintenance of an insemination set 4.1. Preparation of the insemination device 4.2. Description of the insemination device and function of its parts 4.3. Narcotization equipment 5. Sperm collection from drones 6. Instrumental insemination of a queen bee
Author:
Publisher:
ISBN:
Category : Bee culture
Languages : en
Pages : 918
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Author: Harry Hyde Laidlaw
Publisher: Dadant & Sons
ISBN:
Category : Nature
Languages : en
Pages : 148
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Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 576
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Author: William G. Eberhard
Publisher: Princeton University Press
ISBN: 9780691010854
Category : Family & Relationships
Languages : en
Pages : 532
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Book Description
Evidence from various fields indicates that such selectivity by females may be the norm rather than the exception. Because most postcopulatory competition among males for paternity is played out within the bodies of females, female behavior, morphology, and physiology probably often influence male success in these contests, Eberhard draws examples from a diversity of organisms, ranging from ctenophores to scorpions, nematodes to frogs, and crickets to humans.
Author: Carla Mucignat-Caretta
Publisher: CRC Press
ISBN: 1466553413
Category : Medical
Languages : en
Pages : 614
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Book Description
Intraspecific communication involves the activation of chemoreceptors and subsequent activation of different central areas that coordinate the responses of the entire organism—ranging from behavioral modification to modulation of hormones release. Animals emit intraspecific chemical signals, often referred to as pheromones, to advertise their presence to members of the same species and to regulate interactions aimed at establishing and regulating social and reproductive bonds. In the last two decades, scientists have developed a greater understanding of the neural processing of these chemical signals. Neurobiology of Chemical Communication explores the role of the chemical senses in mediating intraspecific communication. Providing an up-to-date outline of the most recent advances in the field, it presents data from laboratory and wild species, ranging from invertebrates to vertebrates, from insects to humans. The book examines the structure, anatomy, electrophysiology, and molecular biology of pheromones. It discusses how chemical signals work on different mammalian and non-mammalian species and includes chapters on insects, Drosophila, honey bees, amphibians, mice, tigers, and cattle. It also explores the controversial topic of human pheromones. An essential reference for students and researchers in the field of pheromones, this is also an ideal resource for those working on behavioral phenotyping of animal models and persons interested in the biology/ecology of wild and domestic species.
Author:
Publisher:
ISBN:
Category : Bee breeding
Languages : en
Pages : 180
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Book Description
