Structural Templates for Protein Design: the Assembly of Peptidyl Motifs with Defined Supersecondary Structure PDF Download
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Author: Mary Denise Struthers
Publisher:
ISBN:
Category :
Languages : en
Pages : 608
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Book Description
Author: Mary Denise Struthers
Publisher:
ISBN:
Category :
Languages : en
Pages : 608
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Book Description
Author: Raphael Guerois
Publisher: Springer Science & Business Media
ISBN: 1597451169
Category : Science
Languages : en
Pages : 303
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Book Description
Protein Design: Methods and Applications presents the most up-to-date protein design and engineering strategies so that readers can undertake their own projects with a maximum chance of success. The authors present integrated computational approaches that require various degrees of computational complexity, and the major accomplishments that have been achieved in the design and structural characterization of helical peptides and proteins.
Author: Norbert Sewald
Publisher: John Wiley & Sons
ISBN: 3527802657
Category : Science
Languages : en
Pages : 771
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Book Description
Der "Sewald/Jakubke" ist und bleibt das einzige moderne, auf dem neuesten wissenschaftlichen Stand gehaltene fortgeschrittene Lehrbuch der Peptid-Biochemie! Auch für diese zweite, um ein Viertel erweitere Auflage haben die Autoren Hunderte von Publikationen gelesen und zu einem informativen und vollständigen Überblick über das Fachgebiet zusammengefasst. Nach einer Einführung in die Grundlagen der Peptidstruktur und -synthese werden die wichtigsten Familien biologisch aktiver Peptide systematisch abgehandelt. Mit interdisziplinärem Anspruch werden dann wichtige Anwendungen aus der Biotechnologie, Pharmazie und Biomedizin besprochen. Selbstverständlich werden auch Themen der Spitzenforschung berücksichtigt, zum Beispiel Pseudopeptide, Peptidmimetika und Aspekte der kombinatorischen Synthese. -- Neu in dieser Auflage sind Fragen und Übungsaufgaben, die das Nacharbeiten von Vorlesungen oder das Selbststudium erleichtern.
Author: Owen Lancaster
Publisher:
ISBN:
Category :
Languages : en
Pages : 224
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Author: Dan Ethan McNamara
Publisher:
ISBN:
Category :
Languages : en
Pages : 355
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Book Description
All aspects of life are controlled by proteins which often assemble into complex arrangements. Research on natural and unnatural protein assemblies enhances our understanding of biological science. The research described here provides structural and mechanistic insights into how protein assemblies could be utilized to improve or develop novel protein functions. Bacterial protein assemblies, microtubule-targeting compounds, and designed protein assemblies were investigated with X-ray crystallography and our results may further the understanding of these targets in pursuit of therapeutics for cancer, obesity, and other conditions. The first study revealed an unexpectedly large structure and putative mechanism for the enzyme DmrB that may help in the design of anti-obesity drugs targeting gut bacteria. A second effort helped to explain how the outer shells of huge bacterial microcompartments may assemble together. The research carried out may help better implement efforts underway to engineer more stable microcompartments for new biotechnology approaches. Structural studies of the interactions which mediate enzyme encapsulation in bacterial microcompartments were also pursued. Our results establish methods for the isolation and subsequent characterization of binary and potentially ternary complexes of microcompartment shell proteins and luminal enzymes. Current tubulin-targeting cancer therapeutics suffer from many drawbacks underscoring the need to further understand the structure of eukaryotic protein assemblies such as microtubules. We describe the structural characterization of two recently-identified compounds that potently kill cancer cell lines. High-throughput screening identified MI-181 and C2 in contexts of mitotic inhibition and cancer cell-death. X-ray crystal structures of MI-181 and C2 bound to a complex containing -tubulin, tubulin tyrosine ligase, and the tubulin-binding protein stathmin revealed details of how these compounds interact with -tubulin subunits. These structures clarify the unknown binding properties and may guide the improvement of these compounds as candidates for therapeutics or chemical probes for better understanding cell division. In addition to natural protein assemblies, unnatural symmetric protein materials can be achieved through protein design. We have solved crystal structures of four computationally-designed protein assemblies. Additional structural studies of designed proteins revealed structures of sequences engineered to form cyclic oligomers and three-dimensional lattice symmetries. These structures emphasize the design accuracy that can be achieved using computational methods in favorable cases. These most recent designed assemblies are built from two distinct components, a strategy that lends itself to versatile construction of functional nanomaterials for a variety of applications. Furthermore, we have developed an additional new method to design two-component protein assemblies which may be engineered for new functions. Fusion of a self-associating heterologous pair of helical elements to symmetric oligomers can be implemented in a way that generates three-dimensional closed systems or extended arrays of protein. Protein engineering applications are proposed and intial designs are described for several biotechnology purposes. Self-assembling protein designs will contain short peptide ligands for cancer-related growth receptors. Designs in which symmetric oligomers self-assemble into extended rod-like filaments were tested for this application. Proper assembly was investigated with electron microscopy after purification from bacteria. The coiled-coil fusion design approach identified candidates for novel nanomaterials which may induce cellular responses related to cell growth and cancer in cultured endothelial cells. Finally, improving the properties of key enzymes can overcome limitations of in vitro metabolic engineering systems. Enzymes for synthesizing isoprene - a starting compound for generating a variety of value-added products - are a useful test-bed. We incorporated a dimeric plant enzyme for isoprene synthesis into the design of a single-layer array of self-assembling symmetric oligomeric enzymes. Two designs are described which enable the isolation of two components separately which may self-assemble upon mixing to form a two-dimensional layer.
Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 712
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Author: Alexander E. Kister
Publisher: Humana Press
ISBN: 9781493991600
Category : Science
Languages : en
Pages : 438
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Book Description
This second edition volume expands on the previous edition with an update on the latest developments in the field and new techniques used to study secondary and supersecondary structures (SSS) in proteins. Chapters in this book discuss topics such as sequence and structural features of different SSS elements; software used for the automatic annotation of secondary structure elements in proteins; the new developments in secondary and SSS prediction and the modern approaches for energy landscape calculation; protein misfolding and amyloid structural formation; analysis of ‘transformer proteins’; discovery of the structure of the hydrophobic nucleus; and discussions of the main principles of protein structures formation. The contributing authors of the volume are the eminent experts in the field of protein research and bioinformatics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Protein Supersecondary Structures: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about the relationship between amino acids sequences and protein structures, the evolution of proteins and the dynamics of protein formation.
Author:
Publisher:
ISBN:
Category : Dissertation abstracts
Languages : en
Pages : 896
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Book Description
Author: Rafael Trindade Maia
Publisher: BoD – Books on Demand
ISBN: 1839628057
Category : Science
Languages : en
Pages : 147
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Book Description
Homology modeling is an extremely useful and versatile technique that is gaining more and more space and demand in research in computational and theoretical biology. This book, “Homology Molecular Modeling - Perspectives and Applications”, brings together unpublished chapters on this technique. In this book, 7 chapters are intimately related to the theme of molecular modeling, carefully selected and edited for academic and scientific readers. It is an indispensable read for anyone interested in the areas of bioinformatics and computational biology. Divided into 4 sections, the reader will have a didactic and comprehensive view of the theme, with updated and relevant concepts on the subject. This book was organized from researchers to researchers with the aim of spreading the fascinating area of molecular modeling by homology.
Author: Cynthia Gibas
Publisher: "O'Reilly Media, Inc."
ISBN: 9781565926646
Category : Computers
Languages : en
Pages : 452
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Book Description
This practical, hands-on guide shows how to develop a structured approach to biological data and the tools needed to analyze it. It's aimed at scientists and students learning computational approaches to biological data, as well as experienced biology researchers starting to use computers to handle data.
