Structural Studies on the Mechanism of Argyrin B and L12 - L11 Ribosomal Protein Interface PDF Download
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Author: Christopher Swanson
Publisher:
ISBN:
Category : Antibiotics
Languages : en
Pages : 0
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Book Description
The mechanism of action of argyrin B and the molecular interactions of L11 and L12 has been undetermined and underrepresented in the research of bacterial translation. This work seeks to examine the mechanism of action of argyrin B by performing in vitro structural studies of its interaction with its specific target protein elongation factor-G. We demonstrate that argyrin B inhibits translation, and allows GTPase activity to proceed, contrary to assumptions made in prior research. We determine that ribosome recycling is the likely step through which argyrin B acts as an antibiotic, since translocation is unaffected by argyrin B and association with post-termination complexes are increased in the presence of argyrin B. We propose that the mechanism of action involves the ratcheting of EF-G 45 ° from a normal binding conformation with the ribosome, which sterically hinders the positioning of loop II of domain IV of EF-G and the cooperativity of EF-G and RRF in interrupting Bridge 2a, the largest intersubunit bridge on the ribosome. Furthermore, we provide preliminary insight on the molecular interactions present between L11 and the L12 C Terminal Domain by performing alanine scanning mutations on select glutamic acid and aspartic acid residues around an electronegatively charged pocket. These mutations while not effecting L11 binding in a tangible way, strongly influenced select GTPase activity.
Author: Christopher Swanson
Publisher:
ISBN:
Category : Antibiotics
Languages : en
Pages : 0
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Book Description
The mechanism of action of argyrin B and the molecular interactions of L11 and L12 has been undetermined and underrepresented in the research of bacterial translation. This work seeks to examine the mechanism of action of argyrin B by performing in vitro structural studies of its interaction with its specific target protein elongation factor-G. We demonstrate that argyrin B inhibits translation, and allows GTPase activity to proceed, contrary to assumptions made in prior research. We determine that ribosome recycling is the likely step through which argyrin B acts as an antibiotic, since translocation is unaffected by argyrin B and association with post-termination complexes are increased in the presence of argyrin B. We propose that the mechanism of action involves the ratcheting of EF-G 45 ° from a normal binding conformation with the ribosome, which sterically hinders the positioning of loop II of domain IV of EF-G and the cooperativity of EF-G and RRF in interrupting Bridge 2a, the largest intersubunit bridge on the ribosome. Furthermore, we provide preliminary insight on the molecular interactions present between L11 and the L12 C Terminal Domain by performing alanine scanning mutations on select glutamic acid and aspartic acid residues around an electronegatively charged pocket. These mutations while not effecting L11 binding in a tangible way, strongly influenced select GTPase activity.
Author: Carl August Luer
Publisher:
ISBN:
Category : Proteins
Languages : en
Pages : 430
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Book Description
Author: Donald K. Alper
Publisher: Canadian-American Center University of Maine
ISBN:
Category : History
Languages : en
Pages : 48
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Book Description
Author: Candace Wellman
Publisher: Washington State University Press
ISBN: 0874223911
Category : History
Languages : en
Pages : 383
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Book Description
Throughout the mid-1800s, outsiders, including many Euro-Americans, arrived in what is now northwest Washington. As they interacted with Samish, Lummi, S’Klallam, Sto:lo, and other groups, some of the men sought relationships with young local women. Hoping to establish mutually beneficial ties, Coast and Interior Salish families arranged strategic cross-cultural marriages. Some pairs became lifelong partners while other unions were short. These were crucial alliances that played a critical role in regional settlement and spared Puget Sound’s upper corner from the tragic conflicts other regions experienced. Accounts of the men, who often held public positions--army officer, Territorial Supreme Court justice, school superintendent, sheriff--exist in a variety of records. Some, like the nephew of Confederate President Jefferson Davis, were from prominent eastern families. Yet across the West, the contributions of their native wives remain unacknowledged. The women’s lives were marked by hardships and heartbreaks common for the time, but the four profiled--Caroline Davis Kavanaugh, Mary Fitzhugh Lear Phillips, Clara Tennant Selhameten, and Nellie Carr Lane--exhibited exceptional endurance, strength, and adaptability. Far from helpless victims, they influenced their husbands and controlled their homes. Remembered as loving mothers and good neighbors, they ran farms, nursed and supported family, served as midwives, and operated businesses. They visited relatives and attended ancestral gatherings, often with their children. Each woman’s story is uniquely hers, but together they and other intermarried women helped found Puget Sound communities and left lasting legacies. They were peace weavers. Author Candace Wellman hopes to shatter stereotypes surrounding these relationships. Numerous collaborators across the United States and Canada--descendants, local historians, academics, and more--graciously participated in her seventeen-year effort.
Author: Aaron Sheehan-Dean
Publisher: Harvard University Press
ISBN: 067491631X
Category : History
Languages : en
Pages : 481
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Book Description
Winner of the Jefferson Davis Award Winner of the Johns Family Book Award Winner of the Army Historical Foundation Distinguished Writing Award “A work of deep intellectual seriousness, sweeping and yet also delicately measured, this book promises to resolve longstanding debates about the nature of the Civil War.” —Gregory P. Downs, author of After Appomattox Shiloh, Chancellorsville, Gettysburg—tens of thousands of soldiers died on these iconic Civil War battlefields, and throughout the South civilians suffered terrible cruelty. At least three-quarters of a million lives were lost during the American Civil War. Given its seemingly indiscriminate mass destruction, this conflict is often thought of as the first “total war.” But Aaron Sheehan-Dean argues for another interpretation. The Calculus of Violence demonstrates that this notoriously bloody war could have been much worse. Military forces on both sides sought to contain casualties inflicted on soldiers and civilians. In Congress, in church pews, and in letters home, Americans debated the conditions under which lethal violence was legitimate, and their arguments differentiated carefully among victims—women and men, black and white, enslaved and free. Sometimes, as Sheehan-Dean shows, these well-meaning restraints led to more carnage by implicitly justifying the killing of people who were not protected by the laws of war. As the Civil War raged on, the Union’s confrontations with guerrillas and the Confederacy’s confrontations with black soldiers forced a new reckoning with traditional categories of lawful combatants and raised legal disputes that still hang over military operations around the world today. In examining the agonizing debates about the meaning of a just war in the Civil War era, Sheehan-Dean discards conventional abstractions—total, soft, limited—as too tidy to contain what actually happened on the ground.
Author: Todd Miller
Publisher: City Lights Books
ISBN: 0872867161
Category : Political Science
Languages : en
Pages : 272
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Book Description
RECIPIENT OF THE 2018 IZZY AWARD FOR EXCELLENCE IN INVESTIGATIVE JOURNALISM "Every so often a book comes along that can dramatically change, or elevate, one's thinking about a global problem. Much like Naomi Klein's books, Todd Miller’s Storming the Wall is such a book and deserves far more attention and discussion."—Izzy Award Judges, Ithaca College *** "A galvanizing forecast of global warming's endgame and a powerful indictment of America's current stance."—Kirkus Reviews As global warming accelerates, droughts last longer, floods rise higher, and super-storms become more frequent. With increasing numbers of people on the move as a result, the business of containing them—border fortification—is booming. In Storming the Wall, Todd Miller travels around the world to connect the dots between climate-ravaged communities, the corporations cashing in on border militarization, and emerging movements for environmental justice and sustainability. Reporting from the flashpoints of climate clashes, and from likely sites of futures battles, Miller chronicles a growing system of militarized divisions between the rich and the poor, the environmentally secure and the environmentally exposed. Stories of crisis, greed and violence are juxtaposed with powerful examples of solidarity and hope in this urgent and timely message from the frontlines of the post-Paris Agreement era. Todd Miller's writings about the border have appeared in the New York Times, Tom Dispatch, and many other places. Praise for Storming the Wall "Nothing will test human institutions like climate change in this century—as this book makes crystal clear, people on the move from rising waters, spreading deserts, and endless storms could profoundly destabilize our civilizations unless we seize the chance to re-imagine our relationships to each other. This is no drill, but it is a test, and it will be graded pass-fail"—Bill McKibben, author Eaarth: Making a Life on a Tough New Planet "As Todd Miller shows in this important and harrowing book, climate-driven migration is set to become one of the defining issues of our time.... This is a must-read book."—Christian Parenti, John Jay College of Criminal Justice, author of Tropic of Chaos: Climate Change and the New Geography of Violence "Todd Miller reports from the cracks in the walls of the global climate security state—militarized zones designed to keep powerful elites safe from poor and uprooted peoples.... Miller finds hope—hope that may not survive in Trumpworld."—Molly Molloy, Research librarian for Latin America and the border at New Mexico State University and creator of "Frontera List" "Miller delivers a prescient and sober view of our increasingly dystopian planet as the impacts of human-caused climate disruption continue to intensify."—Dahr Jamail, award-winning independent journalist, author of The End of Ice "Todd Miller's important book chronicles how existing disparities in wealth and power, combined with the dramatic changes we are causing in this planet's ecosystems, mean either we come together around our common humanity or forfeit the right to call ourselves fully human."—Robert Jensen, author of The End of Patriarchy, Plain Radical, and Arguing for Our Lives
Author: Lauren Beck
Publisher:
ISBN: 9781604978544
Category : Art
Languages : en
Pages : 324
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Book Description
*Includes rare color images. This book demonstrates the evolution of Spain's conceptualisation of its enemies, from biblical and Roman times to the early modern period, and it also illustrates how this transformative discourse became exercised upon Spain by its own enemies in Europe. Each chapter contributes to the study of multiplicity as both a problem to be studied as well as a scholarly methodology that anticipates the structure of the problem. This book is divided into three thematic sections, the first of which establishes the medieval roots for representing Spain's early modern enemies. The two chapters that compose this section respectively explore the naming and visualization of an enemy that was almost entirely Muslim. The second section of this book contains two chapters that explore the textual and visual references to Islam in the Americas during the conquest and early in the period of colonization. The last section of this book contrasts the quality of information conveyed by archival and mass-produced texts. The first of two chapters notes that Muslims indeed did come to the Spanish Americas in the early modern period. The archival research prepared for this chapter contrasts with the mass-produced images and texts in the last chapter, and it is argued that different qualities of information are communicated by mass-produced, and therefore shaped, discourse, rather than by uncirculated, unarticulated texts. That is, out of the archives, a different picture of Islam in the Americas emerges. The final chapter examines how Spanish-authored chronicles became transformed through translation, and with the attachment of new illustrations, into propagandistic tools designed to undermine Spanish conquest and claims on land. This book identifies and illustrates the discourse imposed upon Spain's enemies, and demonstrates how other Europeans used that same discourse to de-Occidentalize, disparage and criticize Spanish activities in the early modern period. Each chapter explores the implications of textual and visual multiplicity while questioning the impact multiplicity has had on the conceptualization of the conquest in more modern times. Scholars of history and literature will appreciate different aspects of this book's arguments. The former will encounter in-depth and copious archival sources about the conquest and its related themes, whereas the latter will enjoy the text-image and literary analysis of those aforementioned sources.
Author: Merrill A. Peterson
Publisher:
ISBN: 9780914516187
Category : Nature
Languages : en
Pages : 0
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Book Description
This field guide sets a new standard for insect identification, making it an indispensable resource to naturalists, educators, gardeners, and others. Engaging and accessible, Pacific Northwest Insects features detailed species accounts, each with a vivid photograph of a living adult, along with information for distinguishing similar species, allowing the reader to identify more than 3,000 species found from southern British Columbia to northern California and as far east as Montana. The book features most of the commonly encountered insects, spiders, scorpions, millipedes, centipedes, and kin in the Pacific Northwest, as well as representatives of an amazing variety of unusual and interesting insects living in the area. After more than a decade of research, reviewing hundreds of thousands of museum specimens and scouring the technical entomological literature, Merrill Peterson has brought together for the first time in a single volume a wealth of information on the region's insect life. Detailed identifying information on over 3,000 species Complete description of 1,200 species Organized by insect group for easy identification Up-to-date taxonomy 1,725 color photos, 50 line drawings, and 2 maps
Author: Sara María Uribe Sánchez
Publisher:
ISBN: 9781934254646
Category : Disappeared persons
Languages : en
Pages : 0
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Book Description
"ANTÍGONA GONZÁLEZ is the story of the search for a body, a specific body, one of the thousands of bodies lost in the war against drug trafficking that began more than a decade ago in Mexico. A woman, Antígona González, attempts to narrate the disappearance of Tadeo, her elder brother. She searches for her brother among the dead. San Fernando, Tamaulipas, appears to be the end of her search."--Provided by publisher.
Author: James Waller
Publisher: Oxford University Press
ISBN: 0199300704
Category : Political Science
Languages : en
Pages : 425
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Book Description
This groundbreaking book from one of the foremost leaders in the field presents a fascinating continuum of research-informed strategies to prevent genocide from ever taking place; to avert further atrocities once mass murder occurs; and to prevent further turmoil once a society learns how to rebuild itself.
