Structural Studies of DNA Polymerases PDF Download
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Author: Karl. E. Zahn
Publisher:
ISBN:
Category :
Languages : en
Pages : 336
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Book Description
Chapter 5 represents a work in progress, providing an overview of our continuing efforts to model human DNA polymerase [theta] (hPOLQ), a specialized polymerase overexpressed in breast cancer cells, among others. By solving a 3.9Å crystal structure, we have already obtained an atomic resolution glimpse of the polymerase active site. This dissertation concludes in chapter 6 with the strategies we will be applying in the near future to extend the resolution of our polymerase [theta] model and compose the next round of questions in the area of research.
Author: Karl. E. Zahn
Publisher:
ISBN:
Category :
Languages : en
Pages : 336
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Book Description
Chapter 5 represents a work in progress, providing an overview of our continuing efforts to model human DNA polymerase [theta] (hPOLQ), a specialized polymerase overexpressed in breast cancer cells, among others. By solving a 3.9Å crystal structure, we have already obtained an atomic resolution glimpse of the polymerase active site. This dissertation concludes in chapter 6 with the strategies we will be applying in the near future to extend the resolution of our polymerase [theta] model and compose the next round of questions in the area of research.
Author: Ulrich Hubscher
Publisher: World Scientific
ISBN: 9814465453
Category : Medical
Languages : en
Pages : 338
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Book Description
Maintenance of the information embedded in the genomic DNA sequence is essential for life. DNA polymerases play pivotal roles in the complex processes that maintain genetic integrity. Besides their tasks in vivo, DNA polymerases are the workhorses in numerous biotechnology applications such as the polymerase chain reaction (PCR), cDNA cloning, genome sequencing, nucleic acids-based diagnostics and in techniques to analyze ancient and otherwise damaged DNA. Moreover, some diseases are related to DNA polymerase defects, and chemotherapy through inhibition of DNA polymerases is used to fight HIV, Herpes and Hepatitis B and C infections. We have recently witnessed the discovery of an abundance of novel DNA polymerases in viruses, bacteria, archaea and eukaryotes with specialized properties whose physiological functions are only beginning to be understood. This book summarizes the current knowledge of these fascinating enzymes. It is intended for a wide audience from basic scientists, to diagnostic laboratories and to clinicians who seek a better understanding of these fascinating enzymes.
Author: Thomas A. Steitz
Publisher: CUP Archive
ISBN: 9780521414890
Category : Science
Languages : en
Pages : 108
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Book Description
In this 1993 text, Nobel Prize winner Professor Steitz reviews the wide-ranging research in structural studies of DNA-binding proteins and their complexes with DNA. The author clearly and concisely describes the uses of techniques in molecular genetics, DNA synthesis, protein crystallography and nuclear magnetic response.
Author: Fang Li
Publisher:
ISBN:
Category :
Languages : en
Pages : 260
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Book Description
Author: Zvi Kelman
Publisher: Frontiers Media SA
ISBN: 2889194558
Category : Biotechnology
Languages : en
Pages : 147
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Book Description
DNA polymerases are core tools for molecular biology including PCR, whole genome amplification, DNA sequencing and genotyping. Research has focused on discovery of novel DNA polymerases, characterization of DNA polymerase biochemistry and development of new replication assays. These studies have accelerated DNA polymerase engineering for biotechnology. For example, DNA polymerases have been engineered for increased speed and fidelity in PCR while lowering amplification sequence bias. Inhibitor resistant DNA polymerase variants enable PCR directly from tissue (i.e. blood). Design of DNA polymerases that efficiently incorporate modified nucleotide have been critical for development of next generation DNA sequencing, synthetic biology and other labeling and detection technologies. The Frontiers in Microbiology Research Topic on DNA polymerases in Biotechnology aims to capture current research on DNA polymerases and their use in emerging technologies.
Author: 林良興
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Author: Andrea Jeanne Berman
Publisher:
ISBN: 9780549064886
Category :
Languages : en
Pages : 166
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Book Description
Sequence-independent DNA binding and the mechanism of translocation in B-family polymerases were examined in DNA substrate complexes of phi29 DNA polymerase. These complexes included the translocated binary complex and the post-insertion pre-translocation ternary complex. Comparison of these structures revealed a mechanism of translocation, different from the mechanism for A-family polymerases, based on the coordinated movement of two conserved tyrosine residues.
Author: Ying Li
Publisher:
ISBN:
Category :
Languages : en
Pages : 214
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Book Description
Author: Timothy David Silverstein
Publisher:
ISBN:
Category :
Languages : en
Pages : 169
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Book Description
