Structural Studies of DNA Conformation and Protein-DNA Interactions Using UV Laser Crosslinking and Raman Spectroscopy PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Structural Studies of DNA Conformation and Protein-DNA Interactions Using UV Laser Crosslinking and Raman Spectroscopy PDF full book. Access full book title Structural Studies of DNA Conformation and Protein-DNA Interactions Using UV Laser Crosslinking and Raman Spectroscopy by Elisabeth Maria Evertsz. Download full books in PDF and EPUB format.
Author: Elisabeth Maria Evertsz
Publisher:
ISBN:
Category : Conformational analysis
Languages : en
Pages : 344
Get Book Here
Book Description
Author: Elisabeth Maria Evertsz
Publisher:
ISBN:
Category : Conformational analysis
Languages : en
Pages : 344
Get Book Here
Book Description
Author: Tom Moss
Publisher: Springer Science & Business Media
ISBN: 1592592082
Category : Science
Languages : en
Pages : 633
Get Book Here
Book Description
Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA-protein interactions.
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 776
Get Book Here
Book Description
Author: Yogambigai Velmurugu
Publisher: Springer
ISBN: 3319451294
Category : Science
Languages : en
Pages : 218
Get Book Here
Book Description
Using a novel approach that combines high temporal resolution of the laser T-jump technique with unique sets of fluorescent probes, this study unveils previously unresolved DNA dynamics during search and recognition by an architectural DNA bending protein and two DNA damage recognition proteins. Many cellular processes involve special proteins that bind to specific DNA sites with high affinity. How these proteins recognize their sites while rapidly searching amidst ~3 billion nonspecific sites in genomic DNA remains an outstanding puzzle. Structural studies show that proteins severely deform DNA at specific sites and indicate that DNA deformability is a key factor in site-specific recognition. However, the dynamics of DNA deformations have been difficult to capture, thus obscuring our understanding of recognition mechanisms. The experiments presented in this thesis uncover, for the first time, rapid (~100-500 microseconds) DNA unwinding/bending attributed to nonspecific interrogation, prior to slower (~5-50 milliseconds) DNA kinking/bending/nucleotide-flipping during recognition. These results help illuminate how a searching protein interrogates DNA deformability and eventually “stumbles” upon its target site. Submillisecond interrogation may promote preferential stalling of the rapidly scanning protein at cognate sites, thus enabling site-recognition. Such multi-step search-interrogation-recognition processes through dynamic conformational changes may well be common to the recognition mechanisms for diverse DNA-binding proteins.
Author: Andrew Arthur Travers
Publisher: Oxford University Press, USA
ISBN: 9780199636914
Category : Medical
Languages : en
Pages : 416
Get Book Here
Book Description
DNA-Protein Interactions is a novel compilation of methods for studying the interactions of proteins with DNA. It is a rapidly advancing research area in which multidisciplinary approaches are especially valuable for solving problems and obtaining a detailed understanding of the molecular regulatory interactions involved. This book covers all the major tools that are required for the study of the large macromolecular enzymatic machines that manipulate DNA, with particular emphasis on biophysical techniques applied to the analysis of transcription and its relation to chromatin structure. Knowledge of basic techniques is assumed, although advances in fundamental fields are covered.
Author:
Publisher:
ISBN:
Category : Dissertation abstracts
Languages : en
Pages : 796
Get Book Here
Book Description
Author: Harald Seitz
Publisher: Springer Science & Business Media
ISBN: 3540481478
Category : Medical
Languages : en
Pages : 212
Get Book Here
Book Description
With contributions by numerous experts
Author: Heinz Staffelbach
Publisher:
ISBN:
Category :
Languages : en
Pages : 284
Get Book Here
Book Description
Author: Marcos Simoes-Costa
Publisher: Springer Nature
ISBN: 107162847X
Category : Science
Languages : en
Pages : 288
Get Book Here
Book Description
This volume details protocols emphasizing systems-level approaches that can be applied to genomic analyses. Chapters detail techniques for optimized application in in vivo systems, spatial, physiological, environmental contexts, imaging-based techniques, single-molecule approaches, CRISPR systems, new genomic approaches, and measurements of kinetics governing. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, DNA-Protein Interactions: Methods and Protocols aims to present genome-wide techniques that will complement the biochemistry-based protocols to aid researchers in their studies.
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 524
Get Book Here
Book Description
