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Author: J. Colin Murrell
Publisher: American Society for Microbiology Press
ISBN: 1555815375
Category : Science
Languages : en
Pages : 508
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Book Description
The ideal starting point for investigating, developing, and implementing stable isotope technologies. • Guides researchers through basic, tested, and proven protocols including DNA, RNA, protein, and phospholipid fatty acid (PLFA) SIP, from concept and history through detailed methodology, troubleshooting, and interpretation to optimal and future uses. • Explores important and emerging applications of SIP in environmental microbiology, ranging from bioremediation and gene mining to carbon tracking and gut microflora function. • Examines explorations of further elegant isotope labeling technologies such as Raman-FISH, NanoSIMS, and isotope arrays. • Serves as a valuable resource for environmental microbiology students and researchers and genomics, biotechnology, and medical microbiology professionals.
Author: J. Colin Murrell
Publisher: American Society for Microbiology Press
ISBN: 1555815375
Category : Science
Languages : en
Pages : 508
Get Book Here
Book Description
The ideal starting point for investigating, developing, and implementing stable isotope technologies. • Guides researchers through basic, tested, and proven protocols including DNA, RNA, protein, and phospholipid fatty acid (PLFA) SIP, from concept and history through detailed methodology, troubleshooting, and interpretation to optimal and future uses. • Explores important and emerging applications of SIP in environmental microbiology, ranging from bioremediation and gene mining to carbon tracking and gut microflora function. • Examines explorations of further elegant isotope labeling technologies such as Raman-FISH, NanoSIMS, and isotope arrays. • Serves as a valuable resource for environmental microbiology students and researchers and genomics, biotechnology, and medical microbiology professionals.
Author: Marc G. Dumont
Publisher: Humana
ISBN: 9781493997237
Category : Science
Languages : en
Pages : 247
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Book Description
This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.
Author: Eugene L. Madsen
Publisher: John Wiley & Sons
ISBN: 1118439589
Category : Science
Languages : en
Pages : 592
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Book Description
New and expanded for its second edition, Environmental Microbiology: From Genomes to Biogeochemistry¸ Second Edition, is a timely update to a classic text filled with ideas, connections, and concepts that advance an in-depth understanding of this growing segment of microbiology. Core principles are highlighted with an emphasis on the logic of the science and new methods-driven discoveries. Numerous up-to-date examples and applications boxes provide tangible reinforcement of material covered. Study questions at the end of each chapter require students to utilize analytical and quantitative approaches, to define and defend arguments, and to apply microbiological paradigms to their personal interests. Essay assignments and related readings stimulate student inquiry and serve as focal points for teachers to launch classroom discussions. A companion website with downloadable artwork and answers to study questions is also available. Environmental Microbiology: From Genomes to Biogeochemistry, Second Edition, offers a coherent and comprehensive treatment of this dynamic, emerging field, building bridges between basic biology, evolution, genomics, ecology, biotechnology, climate change, and the environmental sciences.
Author: Harry Gilbert
Publisher: Academic Press
ISBN: 0124159311
Category : Medical
Languages : en
Pages : 564
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Book Description
Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyse cellulolysis. This volume covers subjects such as 'the DNSA reducing assay for measuring cellulases', 'measuring processivity' and 'in situ cellulose detection with carbohydrate-binding modules'.
Author: Jonathan P. Zehr
Publisher: Springer Nature
ISBN: 303067746X
Category : Science
Languages : en
Pages : 191
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Book Description
This book aims to serve as a centralized reference document for students and researchers interested in aspects of marine nitrogen fixation. Although nitrogen is a critical element in both terrestrial and aquatic productivity, and nitrogen fixation is a key process that balances losses due to denitrification in both environments, most resources on the subject focuses on the biochemistry and microbiology of such processes and the organisms involved in the terrestrial environment on symbiosis in terrestrial systems, or on largely ecological aspects in the marine environment. This book is intended to provide an overview of N2 fixation research for marine researchers, while providing a reference on marine research for researchers in other fields, including terrestrial N2 fixation. This book bridges this knowledge gap for both specialists and non-experts, and provides an in-depth overview of the important aspects of nitrogen fixation as it relates to the marine environment. This resource will be useful for researchers in the specialized field, but also useful for scientists in other disciplines who are interested in the topic. It would provide a possible text for upper division classes or graduate seminars.
Author: Javid Ahmad Parray
Publisher: Springer Nature
ISBN: 3031210794
Category : Science
Languages : en
Pages : 381
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Book Description
The book provides an overview relevant to various biological mechanisms that regulate carbon exchanges between the major components and their response to climate change. Climate change has a significant impact on people's lives, energy demand, food security, etc. The soil microbial ecology is vital for assessing terrestrial and aquatic carbon cycles and climate feedback. However, the primary concern is the complexity of the soil microbial community and its severely affected functions due to the climate and other global changes. Global warming comprises an assessment of the dynamic interactions and feedback between microbes, plants, and their physical environment due to climate change. The book will address the need to use a multifactor experimental approach to understand how soil microorganisms and their activities adapt to climate change and the implications of carbon cycle feedback. The most pressing concern is a clearer understanding of the biological factors that regulate carbon exchanges between land, oceans, and the atmosphere and how these exchanges will respond to climate change via climate–ecosystem feedbacks, which could augment or quell regional and global climate change. Terrestrial ecosystems play an important role in climate feedback as they produce and absorb greenhouse gases like carbon dioxide, methane, and nitrous oxides. They also strongly contribute to storing enormous amounts of carbon in living vegetation and soils, rendering them a significant global carbon sink. If climate change projections are realistic, such a rapid increase in carbon loss from soil could exacerbate the soil carbon cycle feedback. The book will determine the role of microbial feedback in regulating soil-land-atmosphere carbon exchange under changing climatic conditions at the regional and global levels. The current book will also focus on recent research designed to use beneficial microbes such as plant growth-promoting microorganisms, fungi, endophytic microbes, and others to improve understanding of the interaction and their potential role in promoting advanced management for sustainable agricultural solutions. Understanding the influence on the native microbiome, such as the distribution of methanogens and methanotrophs, nutritional content, microbial biomass, and other factors, is becoming increasingly crucial to establishing climate-resilient agriculture.
Author: Wolfgang R. Streit
Publisher: Methods in Molecular Biology
ISBN: 9781493982745
Category : Medical
Languages : en
Pages : 320
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Book Description
Author: H. Griffiths
Publisher: Garland Science
ISBN: 1000144631
Category : Science
Languages : en
Pages : 532
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Book Description
In this authoritative review, leading international researchers explore the growing range of applications of stable isotope techniques for probing and integrating biological processes and palaeoclimatic cycles. The interdisciplinary approach covers a wide range of issues, opportunities and developments, setting interactions with plants in the context of water and nutrient cycles, exchanges with the atmosphere and modelling past and present climate change. This important book will appeal to those requiring an overview of the use of stable isotopes in aquatic, terrestrial and climatic processes and is in tune with current global concerns. In addition postgraduates and research scientists will find an extensive guide to more specialist disciplines, including developing mass spectrometer technologies, compound-specific and cellular-discrimination processes or whole organism and ecosystem responses.
Author: G.D. Birnie
Publisher: Butterworth-Heinemann
ISBN: 1483278417
Category : Science
Languages : en
Pages : 340
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Book Description
Centrifugal Separations in Molecular and Cell Biology focuses on the application of modern centrifugation technology in molecular and cell biology, including the separation and fractionation of biological particles by centrifugation on the preparative and analytical scales. The selection first covers the principles and practices of centrifugation and the bases of centrifugal separations. Discussions focus on the basic concepts of sedimentation theory, centrifugation methods, designing centrifugation experiments, care of centrifuges and rotors, and statistical estimation of molecular parameters. The book also ponders on the practical aspects of rate-zonal centrifugation, including gradient materials, density and viscosity of glycerol solutions, and resolution and gradient shape. The publication examines fractionations in zonal rotors and the quantitative aspects of rate-zonal centrifugation. The text then reviews isopycnic centrifugation in ionic media and analytical centrifugation. Topics include separation by isopycnic banding, large-scale preparative procedures, and density-gradient solutes. The selection is a valuable reference for readers interested in centrifugation technology.
Author: Duu-Jong Lee
Publisher: Elsevier
ISBN: 0444636765
Category : Science
Languages : en
Pages : 526
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Book Description
Current Developments in Biotechnology and Bioengineering: Biological Treatment of Industrial Effluents provides extensive coverage of new developments, state-of-the-art technologies, and potential future trends in data-based scientific knowledge and advanced information on the role and application of environmental biotechnology and engineering in the treatment of industrial effluents. These treatment processes have been broadly classified under aerobic and anaerobic processes which determines the scope and level of pollutant removal. Chapters in this volume review the most recent developments and perspectives at different environmental cleanup operation scales. - Outlines available biochemical processes for the treatment of solid industrial waste - Covers aerobic and anaerobic treatments, their mechanisms, and selection criteria - Highlights specific industrial applications, such as anammox processes
