Author: Hans Christian Walter-Peterson
Publisher:
ISBN:
Category :
Languages : en
Pages : 146
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Book Description
Author: Laila Fayyaz
Publisher:
ISBN: 9781658412230
Category :
Languages : en
Pages :
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Book Description
The growth of the common grapevine (Vitis vinifera L.) has been attempted for about 500 years in the United States. One of the major limitations to its culture is powdery mildew (PM) fungus, Erysiphe necator Schwein, which was first described in eastern North America. Identification and utilization of sources of PM resistance are important for grapevine breeders, which has led to numerous PM resistance studies and evaluations of Vitis germplasm. Our first objective was a wide-scale germplasm screen for foliar resistance to PM focused on accessions of Vitis species from the southwestern United States and northern Mexico. A controlled inoculation method was done with a vacuum-operated settling tower. A total of 147 accessions of 14 Vitis species were evaluated with a broadly virulent isolate of E. necator, the C-strain. Disease symptoms on Vitis vinifera cv. Carignane, a susceptible control, were used to determine the inoculum effect at 14 days post-inoculation. Resistant accessions (nine), moderately susceptible accessions (39), and highly susceptible accessions (99) were observed. Forty-eight accessions were propagated for more intensive study with another fungal isolate, e1-101. The resistant accessions showed consistent results with the e1-101 strain. Geographical differences in susceptibility to PM in species associated with latitudes were not found; across longitudes, western species were generally more susceptible relative to the Midwest and East. No within-species variation was observed in V. monticola and V. treleasei while all other species had intraspecific variation. For interspecific variation, the species V. acerifolia, V. candicans, V. cinerea, and V. X doaniana, possessed significantly more accessions with resistant to moderate susceptibility as compared to V. arizonica, V. berlandieri, V. californica, V. X champinii, V. girdiana, V. riparia, and V. rupestris. This research identified new sources of PM resistance in Vitis from the southwestern US that should be incorporated into PM resistance breeding programs in the US and other grape-growing regions in the world. Our second objective sought to confirm Erysiphe necator (Schw.) resistance in muscadine grapes using in-vitro inoculation. These grapes are believed to be highly resistant to powdery mildew (PM) disease. This study reports on the first comprehensive PM resistance screen in wild and cultivated Muscadinia rotundifolia Michx. Small grapevines (2n=40). A total of 45 M. rotundifolia genotypes (11 cultivated and 34 wild) from the grape collection at the University of California, Davis were inoculated with uniform amounts of PM fungal spores on the young leaves. The sporulating capacity of the two fungal isolates C-strain and the e1-101 strain was observed on each genotype under a microscope. Of the 45 muscadine accessions, 35 showed a consistent disease resistance response to e1-101 strain and C-strain. However, the other 10 accessions, although also resistant, had lower PM resistance scores for the e1-101 strain as compared to C-strain. Flower type of all the accessions was also determined. Seventeen of the 34 wild accessions were male flowered, and 16 were female flowered, one was a hermaphrodite. Five of the cultivated genotypes had female flowers, and 6 were hermaphrodites. The purpose of this screening was to identify new sources of PM resistance, for introgression into V. vinifera. The flower type will help develop the foundation for future studies on the development of hermaphroditic plants, developing genetic markers for plant sex, clarifying the inheritance of sex, and maintaining the muscadine germplasm collection. The third objective was to develop a physical map of the Ren6 PM resistance locus, which was previously identified in the Chinese species, V. piasezkii DVIT2027. The Ren6 resistance locus was found to provide resistance to a broader array of fungal isolates. A map-based cloning approach using bacterial artificial chromosome (BAC) libraries were used to characterize this locus. The physical map helped us identify 13 putative resistance genes at the Ren6 locus between SSR marker PN9-66.01 and CS-07 in susceptible contig 24h16 using Cabernet Sauvignon (CS) reference genome browser (ORF1263bp, ORF1827bp, ORF2910bp, ORF3774bp, ORF3897bp, ORF1212bp, ORF2865bp, ORF1002bp, ORF2982bp, ORF1449bp, ORF2031bp, ORF2790bp, ORF2808bp). Seven of the 13 ORFs were annotated in the CS reference genome. These include ORF1263bp, ORF1827bp, ORF2910bp, ORF1212bp, ORF2865bp, ORF2790bp, ORF2808bp). Evidently, the majority of the annotated ORFs, including ORF2910bp, ORF2808bp, ORF1212bp and ORF2865bp are predicted to code for disease resistance proteins NBS-LRR. Six of the 13 identified ORFs did not have specific annotations on the CS genome or within NCBI following searches against a non-redundant NCBI database - ORF3774bp, ORF3897bp, ORF1002bp, ORF2982bp, ORF1449bp, and ORF2031bp. However, the resistant contig contained three ORFs (ORF2874bp, ORF2700bp, ORF2790bp) between probe Ren6-n2 and SSR marker CS-03, which stretches approximately 30kb in BAC 51e12. This suggested that there are gaps within the resistant contig. Confirming the role of Ren6 in PM resistance requires further investigation involving screening large segregating populations for E. necator resistance, sequencing more positive BAC clones, annotation of candidate resistance gene, validation of putative gene(s) function via transformation into susceptible hosts, and comparisons with other known PM resistance genes both from North America and Asia.
Author: Hans Christian Walter-Peterson
Author: 
Author: Michael Andrew Walker
Author: Michaeleen Doucleff
Author: Michael Andrew Walker
Author: E. P. Botos
Author: Michaeleen Doucleff
Author: 
Author: E. P. Botos