RNA Polymerase-binding Proteins from Sporulating Bacillus Subtilis PDF Download
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Author: Arno Lee Greenleaf
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Arno Lee Greenleaf
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
![Transcriptional Regulation of Genes Encoding Spore Coat Proteins by Mother-cell Specific O{u031B}[superscript K] RNA Polymerase During Bacillus Subtilis Sporulation PDF](https://books.google.com/books/content/images/frontcover/epoDr5G0AIwC?fife=w80-h100)
Author: Hiroshi Travis Ichikawa
Publisher:
ISBN:
Category : Bacillus subtilis
Languages : en
Pages : 232
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Author: David Dubnau
Publisher: Elsevier
ISBN: 032315252X
Category : Nature
Languages : en
Pages : 391
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Book Description
The Molecular Biology of the Bacilli, Volume I: Bacillus subtilis focuses on areas of research traditionally investigated in Bacillus subtilis, as well as topics in which outstanding progress has been made. It discusses the sporulation, defective bacteriophage, and transformation of Bacillus subtilis. Organized into 11 chapters, the book begins with the genetic map of Bacillus subtilis, followed by DNA replication and RNA polymerase of the said species. The book then describes the translational apparatus of Bacillus subtilis. It also explains the genetic transformation in Bacillus subtilis; the sporulation genes; the regulatory mechanisms in the development of lytic bacteriophages in this species; the temperate Bacillus subtilis phages; the specialized transduction in Bacillus subtilis; and molecular cloning in this organism. Lastly, the book considers the most economically important areas of the microbiological industry employing bacilli, including the production of enzymes, nucleosides, riboflavin, and preparations pathogenic to insects. This book will be useful to scientists who are concerned with the use of Bacillus subtilis as a tool for the study of molecular biology and to those who wish to increase the medical, veterinary, and industrial usefulness of this and related organisms.
Author: Uyen Nguyen
Publisher:
ISBN:
Category :
Languages : en
Pages :
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RNA polymerase (RNAP) is an essential enzyme that is responsible for transcription of genes in all organisms. A protein called HelD (YvgS) has been recently identified as a transcription factor of RNAP in Gram-positive Bacillus subtilis, which enhances transcription by increasing RNAP recycling. Based on sequence homology, HelD belongs to the Superfamily 1 (SF1) DNA helicase. However, the mechanism of HelD-dependent transcription activation is limited due to lack of the structures of HelD and also the RNAP and HelD complex. The goal of this project is to obtain a high-resolution three-dimensional structure of HelD in complex with B. subtilis RNAP to elucidate the structural basis of RNAP recycling by the DNA helicase HelD. Two techniques have been used toward the structure determinations in this project: one is X-ray crystallography to determine the structure of HelD, and single-particle cryo-electron microscopy (cryo-EM) to determine the structure of HelD-RNAP complex. Truncations of HelD were constructed for protein expression to enhance protein crystallibility. DNA binding and unwinding function were assayed with HelD using electrophoretic mobility shift assay to ensure functional HelD was purified for downstream structural determination experiments. Overall, this project provides a framework for purification protocol of HelD. In future experiments, structure determination of HelD and HelD- RNAP complex would enhance our knowledge of bacterial transcription.
Author: Jan Löwe
Publisher: Springer
ISBN: 331953047X
Category : Science
Languages : en
Pages : 457
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This book describes the structures and functions of active protein filaments, found in bacteria and archaea, and now known to perform crucial roles in cell division and intra-cellular motility, as well as being essential for controlling cell shape and growth. These roles are possible because the cytoskeletal and cytomotive filaments provide long range order from small subunits. Studies of these filaments are therefore of central importance to understanding prokaryotic cell biology. The wide variation in subunit and polymer structure and its relationship with the range of functions also provide important insights into cell evolution, including the emergence of eukaryotic cells. Individual chapters, written by leading researchers, review the great advances made in the past 20-25 years, and still ongoing, to discover the architectures, dynamics and roles of filaments found in relevant model organisms. Others describe one of the families of dynamic filaments found in many species. The most common types of filament are deeply related to eukaryotic cytoskeletal proteins, notably actin and tubulin that polymerise and depolymerise under the control of nucleotide hydrolysis. Related systems are found to perform a variety of roles, depending on the organisms. Surprisingly, prokaryotes all lack the molecular motors associated with eukaryotic F-actin and microtubules. Archaea, but not bacteria, also have active filaments related to the eukaryotic ESCRT system. Non-dynamic fibres, including intermediate filament-like structures, are known to occur in some bacteria.. Details of known filament structures are discussed and related to what has been established about their molecular mechanisms, including current controversies. The final chapter covers the use of some of these dynamic filaments in Systems Biology research. The level of information in all chapters is suitable both for active researchers and for advanced students in courses involving bacterial or archaeal physiology, molecular microbiology, structural cell biology, molecular motility or evolution. Chapter 3 of this book is open access under a CC BY 4.0 license.
Author: Phillip Kent Freese
Publisher:
ISBN:
Category :
Languages : en
Pages : 298
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Author: Richard Brott Halberg
Publisher:
ISBN:
Category : Bacillus subtilis
Languages : en
Pages : 474
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Author: Kurt Lawrence Fredrick
Publisher:
ISBN:
Category :
Languages : en
Pages : 270
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Author: Cynthia Dianne Dickel
Publisher:
ISBN:
Category :
Languages : en
Pages : 324
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Author: Ronald Wayne Davis
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 272
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