RNA Binding to RNA Polymerase II Purified from the Yeast Saccharomyces Cerevisiae PDF Download
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Author: Tracy Lanise Johnson
Publisher:
ISBN:
Category :
Languages : en
Pages : 400
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Author: Tracy Lanise Johnson
Publisher:
ISBN:
Category :
Languages : en
Pages : 400
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Author: Rodney Gerard Weilbaecher
Publisher:
ISBN:
Category :
Languages : en
Pages : 536
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Author: Rachel Imoberdorf
Publisher:
ISBN:
Category :
Languages : en
Pages : 130
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Transcription occurs in a repressive chromatin context. Chromatin modifications are therefore often required to allow transcriptional activation. Here, we addressed the question whether global histone acetylation is involved in the regulation of transcriptional activation of class II promoters. We show that global histone acetylation has a role in activation of RNA polymerase II dependent transcription by modulating the inherent inhibitory effects of chromatin on the formation of the pre-initiation complex. In a second project we have developed a chromatin immunoprecipitation based assay that allows the study of transcriptional reinitiation "in vivo". Our results suggest the formation of a reinitiation scaffold on the GAL1, but not the ACT1 promoter. In a third project assessed whether TFIIB reaches promoters in the form of the holoenzyme "in vivo". Our data indicate that TFIIB does not assemble in a holoenzyme but associates with another, yet unidentified, factor(s) prior to promoter binding.
Author: Gavin David Meredith
Publisher:
ISBN:
Category :
Languages : en
Pages : 310
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Author: Lei Zhang
Publisher:
ISBN:
Category : Messenger RNA.
Languages : en
Pages : 288
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Author: John Jun Kang
Publisher:
ISBN:
Category :
Languages : en
Pages : 444
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Author: Susanna Tronnersjö
Publisher:
ISBN: 9789157672582
Category :
Languages : en
Pages : 68
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Author: Karen Renee Christie
Publisher:
ISBN:
Category :
Languages : en
Pages : 600
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Book Description
Regulation of the process of transcriptional elongation is an important control mechanism in the expression of some genes. To fully understand this form of regulation will require better understanding of the functions of transcription elongation factors. The goal of this work was to characterize the transcription elongation factor TFIIS from Saccharomyces cerevisiae, originally called P37. I demonstrated that, like the mammalian TFIIS proteins, the yeast protein stimulates RNA polymerase II to cleave the nascent RNA transcript and to read-through an intrinsic block to elongation. Investigation of the protein-protein contacts between TFIIS and RNA polymerase II indicated that the carboxyl-terminal domain of the largest subunit, subunit four, and subunit seven of the polymerase are not required for TFIIS to promote cleavage and read-through by the polymerase. In addition the carboxyl-terminal half of the yeast TFIIS protein is sufficient for both of these in vitro activities. This result is consistent with the previous results demonstrating the carboxyl-terminus of mouse TFIIS was sufficient to activate RNA polymerase in vitro.
Author: Jason Nicholas Kuehner
Publisher:
ISBN:
Category :
Languages : en
Pages : 188
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Author: David C. Amberg
Publisher: CSHL Press
ISBN: 0879697288
Category : Genetics
Languages : en
Pages : 250
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Book Description
"Methods in Yeast Genetics" is a course that has been offered annually at Cold Spring Harbor for the last 30 years. This provides a set of teaching experiments along with the protocols and recipes for the standard techniques and reagents used in the study of yeast biology.
