Reconstitution of Mammalian Ion Channels in Droplet Bilayers PDF Download
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Author: Abha Jeurkar
Publisher:
ISBN:
Category :
Languages : en
Pages : 61
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Book Description
Ion channel studies are important for scientific characterization of cellular processes and for the purpose of drug discovery. The sessile droplet bilayer platform allows for simple, fast, inexpensive and high-yield formation of artificial lipid bilayers and reconstitution of ion channels. We validated this bilayer formation platform with mammalian ion channels like Chloride Intracellular Channel 1 (CLIC1) and Voltage-gated potassium channel 1.2 (Kv1.2). Ion channel synthesis using traditional bacterial expression systems is a complex and time consuming process and it limits the number of eukaryotic proteins that can be expressed therein. We explored the use of in vitro protein synthesis and plasma membrane fractionation of commercially available mammalian cells for expressing and reconstituting ion channel proteins in planar lipid bilayers.
Author: Abha Jeurkar
Publisher:
ISBN:
Category :
Languages : en
Pages : 61
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Book Description
Ion channel studies are important for scientific characterization of cellular processes and for the purpose of drug discovery. The sessile droplet bilayer platform allows for simple, fast, inexpensive and high-yield formation of artificial lipid bilayers and reconstitution of ion channels. We validated this bilayer formation platform with mammalian ion channels like Chloride Intracellular Channel 1 (CLIC1) and Voltage-gated potassium channel 1.2 (Kv1.2). Ion channel synthesis using traditional bacterial expression systems is a complex and time consuming process and it limits the number of eukaryotic proteins that can be expressed therein. We explored the use of in vitro protein synthesis and plasma membrane fractionation of commercially available mammalian cells for expressing and reconstituting ion channel proteins in planar lipid bilayers.
Author: C. Miller
Publisher: Springer Science & Business Media
ISBN: 1475713614
Category : Science
Languages : en
Pages : 579
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Book Description
It is now over 30 years since the idea of ion-conducting pores burst on the elec trophysiological scene, 15 years since these were generalIy realized to be mem brane-spanning proteins, and 10 years since the first observations of single ion channels from higher organisms were made. During the past 5 years, several integral membrane channel proteins have been purified in a functionalIy competent state: the nicotinic acetylcholine receptor, the Na + channel, mitochondrial "VDAC," and a variety of porins. The stage is thus set to attack ion channels in the same ways that biochemists have been attacking enzymes for decades: isolation folIowed by functional analysis in as simple a system as possible, with a view towards understanding the molecular mechanisms ofthe protein's behavior and how this is related to the underlying molecular structure. This is always a daunting task, alI the more so with ion channels because of our still primitive and scanty understanding of channel structures and because of the difficulty in iso lating functionally active channel proteins. In this volume, which can be considered a biochemically slanted companion to Sakmann and Neher's Single-Channel Recording, I have tried to present a view of the current landscape of ion-channel reconstitution. These chapters illustrate not only the different approaches and techniques of the major practitioners of ion channel reconstitution but, as importantly, the varied motivations for doing this kind of work.
Author:
Publisher: Academic Press
ISBN: 0323853757
Category : Science
Languages : en
Pages : 358
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Book Description
Ion Channels Part A, Volume 651 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of new developments on the topic. Each chapter is written by an international board of authors. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series
Author: Masahito Yamagata
Publisher: Frontiers Media SA
ISBN: 2889711498
Category : Science
Languages : en
Pages : 294
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Book Description
Author: Ruth Eatock
Publisher: Springer Science & Business Media
ISBN: 0387317066
Category : Science
Languages : en
Pages : 465
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Book Description
The Springer Handbook of Auditory Research presents a series of compreh- sive and synthetic reviews of the fundamental topics in modern auditory - search. The volumes are aimed at all individuals with interests in hearing research including advanced graduate students, postdoctoral researchers, and clinical investigators. The volumes are intended to introduce new investigators to important aspects of hearing science and to help established investigators to better understand the fundamental theories and data in ?elds of hearing that they may not normally follow closely. Each volume presents a particular topic comprehensively, and each serves as a synthetic overview and guide to the literature. As such, the chapters present neither exhaustive data reviews nor original research that has not yet appeared in peer-reviewed journals. The volumes focus on topics that have developed a solid data and conceptual foundation rather than on those for which a literature is only beginning to develop. New research areas will be covered on a timely basis in the series as they begin to mature.
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Book Description
Author:
Publisher: Academic Press
ISBN: 0128096217
Category : Science
Languages : en
Pages : 350
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Book Description
Piezo Channels, Volume 79, the latest volume in the Current Topics in Membranes series provides the necessary membrane research to assist readers in discovering the current state of a particular field and future directions. New chapters in the updated volume include A Tour de Force: The Discovery, Properties, and Function of Piezo Channels, Piezo1 Channels in Vascular Development and the Sensing of Shear Stress, the Origin of the Force: The Force-From-Lipids Principle Applied to Piezo Channels, Genetic Diseases of PIEZO1 and PIEZO2 Dysfunction, and The Structural Basis for Sensing by the Piezo1 Protein. Users of this series will find an up-to-date presentation of the current knowledge in the field of Piezo Channels. Written by leading experts in the field Contains original material, both textual and illustrative, that make it a very relevant reference Presented in a very comprehensive manner Ideal reference for both researchers in the field and general readers who will find this book to be relevant and up-to-date
Author: Richard M. Epand
Publisher: Springer
ISBN: 9811062447
Category : Science
Languages : en
Pages : 224
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Book Description
This volume focuses on the modulation of biological membranes by specific biophysical properties. The readers are introduced to emerging biophysical approaches that mimick specific states (like membrane lipid asymmetry, membrane curvature, lipid flip-flop, lipid phase separation) that are relevant to the functioning of biological membranes. The first chapter describes innovative methods to mimic the prevailing asymmetry in biological membranes by forming asymmetrical membranes made of monolayers with different compositions. One of the chapters illustrates how physical parameters, like curvature and elasticity, can affect and modulate the interactions between lipids and proteins. This volume also describes the sensitivity of certain ion channels to mechanical forces and it presents an analysis of how cell shape is determined by both the cytoskeleton and the lipid domains in the membrane. The last chapter provides evidence that liposomes can be used as a minimal cellular model to reconstitute processes related to the origin of life. Each topic covered in this volume is presented by leading experts in the field who are able to present clear, authoritative and up-to-date reviews. The novelty of the methods proposed and their potential for a deeper molecular description of membrane functioning are particularly relevant experts in the areas of biochemistry, biophysics and cell biology, while also presenting clear and thorough introductions, making the material suitable for students in these fields as well.
Author: Yasunobu Okada
Publisher: Springer
ISBN: 9784431539926
Category : Medical
Languages : en
Pages : 0
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Book Description
Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.
Author: Ales Iglic
Publisher: Academic Press
ISBN: 0123965349
Category : Medical
Languages : en
Pages : 358
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Book Description
Advances in Planar Lipid Bilayers and Liposomes volumes cover a broad range of topics, including main arrangements of the reconstituted system, namely planar lipid bilayers as well as spherical liposomes. The invited authors present the latest results of their own research groups in this exciting multidisciplinary field. Incorporates contributions from newcomers and established and experienced researchers Explores the planar lipid bilayer systems and spherical liposomes from both theoretical and experimental perspectives Serves as an indispensable source of information for new scientists
