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Author: Per Lundahl
Publisher: Routledge
ISBN: 1317836545
Category : Science
Languages : en
Pages : 244
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Book Description
First published in 2004, this book collects several up-to-date methods for quantitative analysis of biospecific interactions, a field that has a long history that perhaps can be said to have begun with the classical paper of G. Scatchard in 1949 (The attractions of proteins for small molecules and ions, but which has advanced impressively during the last few years. A precise spatial arrangement of just a few hydrogen bonds can confer a remarkably specific reversible association between two molecules. A web of weak interactions governs biospecific recognition in general. The binding equilibria in living cells tune and coordinate a multitude of functions. The thermodynamic properties of such interactions are often studied by binding experiments in simplified and essentially ideal systems. However, similar types of studies may elucidate the biologically relevant dynamic steady-state conditions in living cells and organisms, allowing for the very wide range of interactant concentrations and the interplay between the many reactions and interactions. The development in biosciences will continue with in-depth studies of macromolecules and membranes. More detailed knowledge will allow analyses of delicate balances between substances and events in the complex systems involved in life processes. Methods to study biospecific affinities are thus highly important tools for understanding mechanisms and effects of molecular binding events in vivo and in vitro, e.g., in biochemical, biomedical and pharmaceutical research, and for biotechnological research and production.
Author: Per Lundahl
Publisher: Routledge
ISBN: 1317836545
Category : Science
Languages : en
Pages : 244
Get Book Here
Book Description
First published in 2004, this book collects several up-to-date methods for quantitative analysis of biospecific interactions, a field that has a long history that perhaps can be said to have begun with the classical paper of G. Scatchard in 1949 (The attractions of proteins for small molecules and ions, but which has advanced impressively during the last few years. A precise spatial arrangement of just a few hydrogen bonds can confer a remarkably specific reversible association between two molecules. A web of weak interactions governs biospecific recognition in general. The binding equilibria in living cells tune and coordinate a multitude of functions. The thermodynamic properties of such interactions are often studied by binding experiments in simplified and essentially ideal systems. However, similar types of studies may elucidate the biologically relevant dynamic steady-state conditions in living cells and organisms, allowing for the very wide range of interactant concentrations and the interplay between the many reactions and interactions. The development in biosciences will continue with in-depth studies of macromolecules and membranes. More detailed knowledge will allow analyses of delicate balances between substances and events in the complex systems involved in life processes. Methods to study biospecific affinities are thus highly important tools for understanding mechanisms and effects of molecular binding events in vivo and in vitro, e.g., in biochemical, biomedical and pharmaceutical research, and for biotechnological research and production.
Author: Zhuangzhi Wang
Publisher:
ISBN:
Category : DNA-protein interactions
Languages : en
Pages : 380
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Book Description
Author: Toni Kline
Publisher: CRC Press
ISBN: 1482285010
Category : Science
Languages : en
Pages : 1198
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Book Description
Outlining the fundamental principles by which all interactions occur, this reference focuses on harnessing the biochemistry of bioorganic compounds in order to separate them, presenting new techniques and applications that affect the planning of research strategies. The contributors discuss how to c
Author: M.A. Vijayalakshmi
Publisher: Springer Science & Business Media
ISBN: 9400911076
Category : Science
Languages : en
Pages : 343
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Book Description
This volume contains the papers and reports presented at the First International Conference on Dye-Protein Interaction, held 24-28 July 1988 at the University of Compiegne, France. This was the first international meeting dealing entirely with dye-protein interaction. The major focus of the conference was on the better understanding of the mechanism of interaction of proteins with different triazine dyes and the synthesis of novel structural dyes having good biomimetic activities. The potentials and limits of their use in biotechnology, mainly for purification, were stressed. Current contributions in developing dye-based affinity methods were highlighted in such areas as affinity partition, affinity precipitation and new support matrices for efficient affinity chromatography, etc. The interrelation between metal chelates and dyes in terms of their interactions with proteins was underlined. It is our belief that this proceedings volume will be a stimulus for broad and creative applications of dye affinity concepts in many fields of biomedical research and biotechnology. for understanding the In addition, a discussion session emphasised the necessity toxicological aspects of these dyes, their fragments and their metabolites. This helped to trigger plans for future work, and this topic will be one of the priorities in a future meeting on dye-protein interactions. C. R. The help of the International Scientific Committee, which included Drs Lowe (UK), G. KopperschHiger (GDR) , E. Stellwagen (USA), D. Thomas (France), G. Birkenmeier (GDR), S. Rajgopal-Narayan (USA), J. P. Dandeu (France), D. Muller (France) and E. Dellacherie (France), in organising this meeting is gratefully acknowledged.
Author: Electra Gizeli
Publisher: CRC Press
ISBN: 0203212193
Category : Medical
Languages : en
Pages : 337
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Book Description
The development of devices that incorporate biological assemblies is impacting analytical and biomedical research. Today, scientists can monitor vital biological interactions-such as the binding of DNA to proteins-in real time, deriving unique information necessary to understanding biochemical pathways and thus aiding the design of drugs to regulat
Author: Ziad El Rassi
Publisher: Elsevier
ISBN: 0128245123
Category : Science
Languages : en
Pages : 956
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Book Description
Carbohydrate Analysis by Modern Liquid Phase Separation Techniques, Second Edition, presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates. In a selection of all-new chapters, this fully updated volume covers each technique in detail. The book aims to help analysts solve any of the many practical problems they may face in tackling the analysis of carbohydrates. In addition, it addresses current difficulties that must be resolved in carbohydrate research, thus inspiring further important technological developments to meet these challenges. This is an essential resource for anyone seeking a broad view of the science of carbohydrates and separation techniques. - Covers the basic principles of modern liquid phase separation techniques, along with their applications - Compiles up-to-date information on the field of carbohydrate analysis, along with updates on separation science - Focuses on problems currently faced in carbohydrate analysis and the solutions necessary for further progress
Author: Aleksandra Sudarić
Publisher: BoD – Books on Demand
ISBN: 9533072407
Category : Science
Languages : en
Pages : 530
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Book Description
The book Soybean: Molecular Aspects of Breeding focuses on recent progress in our understanding of the genetics and molecular biology of soybean and provides a broad review of the subject, from genome diversity to transformation and integration of desired genes using current technologies. This book is divided into four parts (Molecular Biology and Biotechnology, Breeding for Abiotic Stress, Breeding for Biotic Stress, Recent Technology) and contains 22 chapters.
Author: Leonardo Sepúlveda Torre
Publisher: CRC Press
ISBN: 1000565491
Category : Technology & Engineering
Languages : en
Pages : 292
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Book Description
This volume provides up-to-date and detailed scientific information on recent developments and new approaches in food microbiology, focusing on microbial food pathogens. The volume presents the fundamental aspects of food and microorganisms, and also addresses food systems and measures to prevent and control food, foodborne diseases, etc. According to the editors, every minute, there are about 50,000 cases of gastrointestinal diseases from food-mediated infections and food poisoning, and many individuals, especially children, die from these infections. The most important preventive measures are for the development and continuous implementation of effective interventions to improve overall food safety. The book helps to meet the challenge of food safety issues by focusing on the fundamental aspects of food and microorganisms. Each section consists of detailed information on the particular aspects of each topic, including basic microbiology, safety, pathogenic microorganisms, food conservation, sanitization, and hygiene procedures. The microbial diversity found in food is described from the classification by kingdoms and the main groups of microorganisms present in them. Although the main issue is microbial food pathogens, the book also covers another important aspect of food microbiology: food systems and measurements to prevent and control food, foodborne diseases, etc. Quantitative Methods Quantitative Methods and Analytical Techniques in Food Microbiology: Challenges and Health Implications will be a valuable resource for scientists, researchers, faculty, students, and others in various sectors in food science and technology. The scope of food microbiology is highly inclusive, as it interacts with all subdisciplines of microbiology, such as public health microbiology, microbial genetics, fermentation technologies, microbial physiology and biochemistry, and food microbiologists have been at the forefront of many microbiological concepts and advances.
Author: Peter Schuck
Publisher: Springer Science & Business Media
ISBN: 0387359664
Category : Science
Languages : en
Pages : 537
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Book Description
This volume successfully and clearly examines how biophysical approaches can be used to study complex systems of reversibly interacting proteins. It deals with the methodology behind the research and shows how to synergistically incorporate several methodologies for use. Each chapter treats and introduces the reader to different biological systems, includes a brief summary of the physical principles, and mentions practical requirements.
Author: Asif Iqbal Zia
Publisher: Springer
ISBN: 3319326554
Category : Technology & Engineering
Languages : en
Pages : 157
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Book Description
This book describes a robust, low-cost electrochemical sensing system that is able to detect hormones and phthalates – the most ubiquitous endocrine disruptor compounds – in beverages and is sufficiently flexible to be readily coupled with any existing chemical or biochemical sensing system. A novel type of silicon substrate-based smart interdigital transducer, developed using MEMS semiconductor fabrication technology, is employed in conjunction with electrochemical impedance spectroscopy to allow real-time detection and analysis. Furthermore, the presented interdigital capacitive sensor design offers a sufficient penetration depth of the fringing electric field to permit bulk sample testing. The authors address all aspects of the development of the system and fully explain its benefits. The book will be of wide interest to engineers, scientists, and researchers working in the fields of physical electrochemistry and biochemistry at the undergraduate, postgraduate, and research levels. It will also be highly relevant for practitioners and researchers involved in the development of electromagnetic sensors.
