Purification of Peptides in High-Complexity Arrays PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Purification of Peptides in High-Complexity Arrays PDF full book. Access full book title Purification of Peptides in High-Complexity Arrays by Christopher Schirwitz. Download full books in PDF and EPUB format.
Author: Christopher Schirwitz
Publisher: Springer Science & Business Media
ISBN: 3319008072
Category : Science
Languages : en
Pages : 138
Get Book
Book Description
Christopher Schirwitz's thesis focuses on improving the quality of in situ synthesized high-complexity peptide micro arrays. Micro arrays containing proteins or small protein fragments in the form of peptides have become of great interest in proteomic research. With the help of these microarrays a large number of potential target molecules can be screened for interaction with a probe in a short timeframe. However, protein and peptide micro arrays are still lagging behind oligonucleotide arrays in terms of density, quality and manufacturing costs. A new approach developed at the German Cancer Research Center (DKFZ) has improved the synthesis of high-density peptide arrays. The current technology is capable of producing arrays with up to 40,000 different peptides per square cm by means of micro particle-based solid phase peptide synthesis. However, in situ synthesis approaches bear a conceptual disadvantage: The quality of the peptides is dependent on the efficiency of the synthesis so that peptide fragments are present in the resulting array among the desired full-length peptides. In peptide-protein interaction studies such peptide fragments. The central achievement of this thesis is the development of a new method allowing for the fast one-step purification of entire arrays without loss of resolution or spatial information. Christopher Schirwitz's work has resulted in a number of publications in high ranking journals.
Author: Christopher Schirwitz
Publisher: Springer Science & Business Media
ISBN: 3319008072
Category : Science
Languages : en
Pages : 138
Get Book
Book Description
Christopher Schirwitz's thesis focuses on improving the quality of in situ synthesized high-complexity peptide micro arrays. Micro arrays containing proteins or small protein fragments in the form of peptides have become of great interest in proteomic research. With the help of these microarrays a large number of potential target molecules can be screened for interaction with a probe in a short timeframe. However, protein and peptide micro arrays are still lagging behind oligonucleotide arrays in terms of density, quality and manufacturing costs. A new approach developed at the German Cancer Research Center (DKFZ) has improved the synthesis of high-density peptide arrays. The current technology is capable of producing arrays with up to 40,000 different peptides per square cm by means of micro particle-based solid phase peptide synthesis. However, in situ synthesis approaches bear a conceptual disadvantage: The quality of the peptides is dependent on the efficiency of the synthesis so that peptide fragments are present in the resulting array among the desired full-length peptides. In peptide-protein interaction studies such peptide fragments. The central achievement of this thesis is the development of a new method allowing for the fast one-step purification of entire arrays without loss of resolution or spatial information. Christopher Schirwitz's work has resulted in a number of publications in high ranking journals.
Author: Christopher Schirwitz
Publisher:
ISBN:
Category : Amino acids
Languages : en
Pages :
Get Book
Book Description
Author: Christopher Schirwitz
Publisher:
ISBN:
Category :
Languages : en
Pages : 191
Get Book
Book Description
Author: Mario Barbosa
Publisher: Woodhead Publishing
ISBN: 008100852X
Category : Technology & Engineering
Languages : en
Pages : 390
Get Book
Book Description
Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair highlights the various important considerations that go into biomaterial development, both in terms of fundamentals and applications. After covering a general introduction to protein and cell interactions with biomaterials, the book discusses proteins in biomaterials that mimic the extracellular matrix (ECM). The properties, fabrication and application of peptide biomaterials and protein-based biomaterials are discussed in addition to in vivo and in vitro studies. This book is a valuable resource for researchers, scientists and advanced students interested in biomaterials science, chemistry, molecular biology and nanotechnology. Presents an all-inclusive and authoritative coverage of the important role which protein and peptides play as biomaterials for tissue regeneration Explores protein and peptides from the fundamentals, to processing and applications Written by an international group of leading biomaterials researchers
Author: Tony Hugli
Publisher: Elsevier
ISBN: 032314425X
Category : Science
Languages : en
Pages : 631
Get Book
Book Description
Techniques in Protein Chemistry compiles reports of methods and techniques presented at the second symposium of the Protein Society in August 1988. This book includes methods and applications in protein sequencing, advanced applications of mass spectrometry and nuclear magnetic resonance technology, limitations of amino acid microanalysis, and advances in high-performance liquid chromatography. The structure of synthetic test peptide-3 (STP-3), a peptide designed to test the analytical limits of current technology in the field of protein chemistry is also elaborated. This publication is suited for chemists and researchers conducting work on the analytical techniques available for the molecular characterization of proteins.
Author: Adriaan Bins
Publisher: Amsterdam University Press
ISBN: 9087280114
Category : Health & Fitness
Languages : en
Pages : 152
Get Book
Book Description
Annotation. In the last century, it has become clear that in patients suffering certain types of cancer, spontaneous activation of the immune sytem may lead to curation. The concept of immunotherapy is based on that notion. The intention is to stimulate T cells directed against the cancer, leading to the eradication of cancer cells. However, the methods to stimulate T cell immunity are just evolving, and at the moment they are functional in small laboratory animals only. Furthermore, we know now that many cancer cells possess the capacity to scive attacks of the immune system. This thesis introduces a novel T cell vaccination method that uses a tattoo machine to inject DNA in the skin of the vaccinee. In comparison to other experimental vaccination methods DNA tattooing is very strong: besides small laboratry animals also large animals (mockeys) mount strong T cell responses on tattoo DNA vaccination. Future tests in melanoma patients will point out whether DNA tattoo vaccination is equally efficient in humans, and whether it may have a therapeutic effect in these patients. This title can be previewed in Google Books - http://books.google.com/books?vid=ISBN9789087280116.
Author: Ved Srivastava
Publisher: Royal Society of Chemistry
ISBN: 1788014332
Category : Science
Languages : en
Pages : 572
Get Book
Book Description
Peptide therapy has become a key strategy in innovative drug development, however, one of the potential barriers for the development of novel peptide drugs in the clinic is their deficiencies in clearly defined chemistry, manufacturing and controls (CMC) strategy from clinical development to commercialization. CMC can often become a rate-limiting step due to lack of knowledge and lack of a formal policy or guidelines on CMC for peptide-based drugs. Regulators use a risk-based approach, reviewing applications on a case-by-case basis. Peptide Therapeutics: Strategy and Tactics for Chemistry, Manufacturing, and Controls covers efficient manufacturing of peptide drug substances, a review of the process for submitting applications to the regulatory authority for drug approval, a holistic approach for quality attributes and quality control from a regulatory perspective, emerging analytical tools for the characterisation of impurities, and the assessment of stability. This book is an essential reference work for students and researchers, in both academia and industry, with an interest in learning about CMC, and facilitating development and manufacture of peptide-based drugs.
Author:
Publisher: John Wiley & Sons
ISBN: 3527631852
Category : Science
Languages : en
Pages : 508
Get Book
Book Description
This is the last of five books in the Amino Acids, Peptidesand Proteins in Organic Synthesis series. Closing a gap in the literature, this is the only series tocover this important topic in organic and biochemistry. Drawingupon the combined expertise of the international "who's who" inamino acid research, these volumes represent a real benchmark foramino acid chemistry, providing a comprehensive discussion of theoccurrence, uses and applications of amino acids and, by extension,their polymeric forms, peptides and proteins. The practical value of each volume is heightened by theinclusion of experimental procedures. The 5 volumes cover the following topics: Volume 1: Origins and Synthesis of Amino Acids Volume 2: Modified Amino Acids, Organocatalysis and Enzymes Volume 3: Building Blocks, Catalysis and Coupling Chemistry Volume 4: Protection Reactions, Medicinal Chemistry,Combinatorial Synthesis Volume 5: Analysis and Function of Amino Acids and Peptides Volume 5 of this series presents a wealth of methods to analyzeamino acids and peptides. Classical approaches are described, suchas X-ray analysis, chromatographic methods, NMR, AFM, massspectrometry and 2D-gel electrophoresis, as well as newerapproaches, including Surface Plasmon Resonance and arraytechnologies. Originally planned as a six volume series, Amino Acids,Peptides and Proteins in Organic Chemistry now completes withfive volumes but remains comprehensive in both scope andcoverage. ahref="http://eu.wiley.com/WileyCDA/WileyTitle/productCd-3527335463.html"Furtherinformation about the 5 Volume Set and purchasing details can beviewed here./a
Author: Colin T. Mant
Publisher: CRC Press
ISBN: 1351440756
Category : Medical
Languages : en
Pages : 960
Get Book
Book Description
This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.
Author: Robert A. Meyers
Publisher: John Wiley & Sons
ISBN: 3527687211
Category : Science
Languages : en
Pages : 1100
Get Book
Book Description
This reference work gives a compete overview of the different stages of drug development using a translational approach. The book is structured in different parts, following the different stages in drug development. Almost half of the work is dedicated to core of drug discovery using a translational approach, the identification of appropriate targets and screening methods for the identification of compounds interacting with these targets. The rest of book covers the whole downstream pipeline after the identification of lead compounds, such as bioavailability issues, identification of appropriate drug delivery venues, production and scaling issues and preclinical trials. As has been the case with other works in the encyclopedia, the book is made up of long, comprehensive and authoritative chapters, written by outstanding researchers in the field.
