Pulsed-field Capillary Electrophoresis of Nucleic Acids PDF Download
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Author: Yongseong Kim
Publisher:
ISBN:
Category :
Languages : en
Pages : 270
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Book Description
Author: Yongseong Kim
Publisher:
ISBN:
Category :
Languages : en
Pages : 270
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Book Description
Author: Christoph Heller
Publisher: Springer Science & Business Media
ISBN: 3322910156
Category : Technology & Engineering
Languages : en
Pages : 318
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Book Description
Die Kapillarelektrophorese (CE) ist heute in biochemischen Laboratorien noch nicht als Routinemethode zur DNA-Trennung etabliert, obwohl sie diverse Vorzüge gegenüber den derzeitigen Methoden besitzt. In diesem Band stellen verschiedene Wissenschaftler die theoretischen Aspekte der Trennung von Oligonucleinsäuren mit der CE, die instrumentellen Grundlagen und deren Grenzen und schließlich die praktische Anwendung in der Biochemie und Molekularbiologie vor. This book on capillary electrophoresis is unique in its focus on the separation of nucleic acids. The importance of electrophoretic separation in every molecular biology laboratory justifies this specialization, which is also reflected in the development of instrumentation. This book is aimed to help to implement this rather new and promising technology in the biological laboratories and to help to overcome typical problems that can occur when starting with a new technique. It should also trigger further development in the field. It covers the theoretical background as well as practical examples of usual applications. The authors are all experts in their field, having many years of experience with capillary electrophoresis and their application to nucleic acids.
Author: Dietmar Tietz
Publisher: Springer Science & Business Media
ISBN: 3642589243
Category : Science
Languages : en
Pages : 341
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Book Description
Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described here in detail. Leading experts present the required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers' addresses, helpful Internet resources, as well as specific applications, e.g. in legal medicine, microbiology and agriculture.
Author: Keith R. Mitchelson
Publisher: Springer Science & Business Media
ISBN: 1592590551
Category : Science
Languages : en
Pages : 481
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Book Description
The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.
Author: Michael James Navin
Publisher:
ISBN:
Category :
Languages : en
Pages : 246
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Book Description
Author: Bruce Birren
Publisher: Academic Press
ISBN: 0323137628
Category : Science
Languages : en
Pages : 272
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Book Description
Pulsed Field Gel Electrophoresis: A Practical Guide is the first laboratory manual to describe the theory and practice of this technique. Based on the authors' experience developing pulsed field gel instruments and teaching procedures, this book provides everything a researcher or student needs to know in order to understand and carry out pulsed field gel experiments. Clear, well-tested protocols assume only that users have a basic familiarity with molecular biology. Thorough coverage of useful data, theory, and applications ensures that this book is also a lasting resource for more advanced practitioners of pulsed field gels. - Reviews all types of pulsed field gel electrophoresis - Describes all commercially available systems and summarizes advantages and limitations of each - Includes step-by-step protocols for sample preparation and analysis - Illustrated with photographs that depict - How to run gels: What the results should look like - What they look like when they go wrong - Covers applications to a wide range of organisms - Includes bibliography of over 900 publications and cross-referenced by topic, application, and organism
Author: Margit Burmeister
Publisher: Springer Science & Business Media
ISBN: 1592594999
Category : Science
Languages : en
Pages : 473
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Book Description
Presents step-by-step protocols for users of Pulsed-Field Gel Electrophoresis (PFGE), whether novice or expert. This book features a wide range of PFGE techniques, auxiliary methods, and a diverse array of powerful applications. It includes protocols designed to work even the first time they are used.
Author: Dietmar Tietz
Publisher:
ISBN: 9783642589256
Category :
Languages : en
Pages : 344
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Book Description
Electrophoresis is a powerful method to analyze nucleic acids (DNA, RNA).
Author:
Publisher: Springer
ISBN: 9781592590551
Category : Capillary electrophoresis
Languages : en
Pages :
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Book Description
Author: David Rickwood
Publisher: Oxford University Press, USA
ISBN:
Category : Language Arts & Disciplines
Languages : en
Pages : 340
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Book Description
The first edition, published in 1982, became the standard source of electrophoresis protocols for nucleic acid researchers. This updated edition takes account of the advances made in refining established techniques and details many new techniques, including pulse field electrophoresis, gel retardation analysis, and DNA footprinting. Annotation copyrighted by Book News, Inc., Portland, OR
