Protein Structure Determination by Paramagnetic NMR and Computational Hybrid Approach PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Protein Structure Determination by Paramagnetic NMR and Computational Hybrid Approach PDF full book. Access full book title Protein Structure Determination by Paramagnetic NMR and Computational Hybrid Approach by Kala Bharath Pilla. Download full books in PDF and EPUB format.
Author: Kala Bharath Pilla
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
Get Book Here
Book Description
Computational modelling of proteins that rely on either de novo or evolutionary based approaches often produce poor quality structures, primarily due to the limitations in their algorithms or forcefields. Traditional experimental techniques such as X-ray crystallography depend on narrow set of crystallographic conditions while solution/solid state nuclear magnetic resonance (NMR) spectroscopy relies on cumbersome spectral analysis and complete resonance assignments. These traditional approaches are slow and costly endeavours. Computational/experimental hybrid approaches on the other hand provide a new avenue for reliable, rapid and cost-effective structure determination. Paramagnetic NMR offers easy generation of useful and sparse structural information which can be implemented as restraints in structure prediction algorithms. Pseudocontact shifts (PCS) are the most powerful of structural restraints generated by paramagnetic NMR which are long range in nature and can be easily obtained by simple 2D NMR experiments. This thesis demonstrates different approaches involved in protein structure calculations using PCS restraints in Rosetta. Chapter 2 demonstrates structure determination using PCS restraints exclusively obtained from protein samples in microcrystalline state by magic angle spinning (MAS) NMR spectroscopy. Chapter 3 discusses the implementation of using PCS data from multiple metal centres to precisely determine the location of spins in space in a manner analogues to GPS-satellites. Chapter 4 extends the usage of PCS data from multiple metal centres to capture distinct conformational states in proteins. Chapter 5 demonstrates new techniques especially developed for structure determination of large proteins involving super secondary structure motifs (Smotifs) and data driven iterative resampling. These different computational techniques serve the goal of determining accurate 3D models using minimal experimental data, which are applicable to proteins systems that are currently beyond the realm of traditional experimental approaches.
Author: Kala Bharath Pilla
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
Get Book Here
Book Description
Computational modelling of proteins that rely on either de novo or evolutionary based approaches often produce poor quality structures, primarily due to the limitations in their algorithms or forcefields. Traditional experimental techniques such as X-ray crystallography depend on narrow set of crystallographic conditions while solution/solid state nuclear magnetic resonance (NMR) spectroscopy relies on cumbersome spectral analysis and complete resonance assignments. These traditional approaches are slow and costly endeavours. Computational/experimental hybrid approaches on the other hand provide a new avenue for reliable, rapid and cost-effective structure determination. Paramagnetic NMR offers easy generation of useful and sparse structural information which can be implemented as restraints in structure prediction algorithms. Pseudocontact shifts (PCS) are the most powerful of structural restraints generated by paramagnetic NMR which are long range in nature and can be easily obtained by simple 2D NMR experiments. This thesis demonstrates different approaches involved in protein structure calculations using PCS restraints in Rosetta. Chapter 2 demonstrates structure determination using PCS restraints exclusively obtained from protein samples in microcrystalline state by magic angle spinning (MAS) NMR spectroscopy. Chapter 3 discusses the implementation of using PCS data from multiple metal centres to precisely determine the location of spins in space in a manner analogues to GPS-satellites. Chapter 4 extends the usage of PCS data from multiple metal centres to capture distinct conformational states in proteins. Chapter 5 demonstrates new techniques especially developed for structure determination of large proteins involving super secondary structure motifs (Smotifs) and data driven iterative resampling. These different computational techniques serve the goal of determining accurate 3D models using minimal experimental data, which are applicable to proteins systems that are currently beyond the realm of traditional experimental approaches.
Author: N. Rama Krishna
Publisher: Springer Science & Business Media
ISBN: 0306470845
Category : Medical
Languages : en
Pages : 565
Get Book Here
Book Description
Volume 17 is the second in a special topic series devoted to modern techniques in protein NMR, under the Biological Magnetic Resonance series. Volume 16, with the subtitle Modern Techniques in Protein NMR , is the first in this series. These two volumes present some of the recent, significant advances in the biomolecular NMR field with emphasis on developments during the last five years. We are honored to have brought together in these volume some of the world s foremost experts who have provided broad leadership in advancing this field. Volume 16 contains - vances in two broad categories: I. Large Proteins, Complexes, and Membrane Proteins and II. Pulse Methods. Volume 17 contains major advances in: I. Com- tational Methods and II. Structure and Dynamics. The opening chapter of volume 17 starts with a consideration of some important aspects of modeling from spectroscopic and diffraction data by Wilfred van Gunsteren and his colleagues. The next two chapters deal with combined automated assignments and protein structure determination, an area of intense research in many laboratories since the traditional manual methods are often inadequate or laborious in handling large volumes of NMR data on large proteins. First, Werner Braun and his associates describe their experience with the NOAH/DIAMOD protocol developed in their laboratory.
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 141
Get Book Here
Book Description
Three-dimensional structures of proteins determined in solution by NMR spectroscopy have the unique advantage of revealing details of molecular structure and dynamics in a physiologically relevant state; however, the many tedious steps needed to solve and validate a structure make this method challenging. The barriers to NMR structure determination become higher for larger proteins whose spectra are harder to resolve. It is clear that advances need to be made in automating protein structure determination by NMR spectroscopy. The goal of my research has been to use computational methods to advance the development of high-throughput NMR spectroscopy. Accelerating and streamlining the structure determination process will enable investigators to spend less time solving structures and more time investigating challenging biomolecular systems. My goals have been to develop an automation protocol that integrates multiple steps, ensures the robustness of each step, incorporates iterative corrections, and includes visualization tools to validate and extend the results. I developed PINE-SPARKY as a graphical interface for checking and extending automated assignments made by the PINE-NMR server. ADAPT-NMR directs fast data collection by reduced dimensionality on the basis of ongoing NMR assignments. I helped develop a version of ADAPT-NMR (originally only for Varian spectrometers) for Bruker spectrometers, and I created ADAPT-NMR Enhancer as a visualization tool for validating and extending assignments made by ADAPT-NMR on either spectrometer system. I developed the PONDEROSA package to automate the next steps. PONDEROSA carries out automatic picking of 3D-NOESY peaks and iterative structure determinations with the protein sequence and the assignments as inputs. These automation and visualization tools cover almost all of the steps involved in protein structure determination by NMR spectroscopy. As a practical test of this technology, I solved the structure of the 2A proteinase from the human rhinovirus. As a side project, I built a relational database (PACSY DB) that combines information from the Protein Data Bank (PDB) and the Biological Magnetic Resonance data Bank (BMRB) and incorporates tools for structure analysis. PACSY DB can carry out complex queries that combine atomic coordinates, NMR parameters, and structural features of proteins.
Author: Daniel Chasman
Publisher: CRC Press
ISBN: 0824748166
Category : Medical
Languages : en
Pages : 534
Get Book Here
Book Description
This text offers in-depth perspectives on every aspect of protein structure identification, assessment, characterization, and utilization, for a clear understanding of the diversity of protein shapes, variations in protein function, and structure-based drug design. The authors cover numerous high-throughput technologies as well as computational methods to study protein structures and residues. A valuable reference, this book reflects current trends in the effort to solve new structures arising from genome initiatives, details methods to detect and identify errors in the prediction of protein structural models, and outlines challenges in the conversion of routine processes into high-throughput platforms.
Author: M. S. Cheung
Publisher:
ISBN:
Category :
Languages : en
Pages :
Get Book Here
Book Description
Author: Haruki Nakamura
Publisher: Springer
ISBN: 9811322007
Category : Science
Languages : en
Pages : 272
Get Book Here
Book Description
This book presents a new emerging concept of "Integrative Structural Biology". It covers current trends of the molecular and cellular structural biology, providing new methods to observe, validate, and keep the structural models of the large cellular machines with recent scientific results. Structures of very large macromolecular machines in cells are being determined by combining observations from complementary experimental methods. Thus, this volume presents the each methods such as X-ray crystallography, NMR spectroscopy, 3DEM, small-angle scattering (SAS), FRET, crosslinking, and enables the readers to understand the hybrid methods. This book discusses how those integrative models should be represented, validated and archived. A unique highlight of this book is discussion of the data validation and archive, which are big problems in this filed along with the progress of this field. The researchers in biology will be interested in this book as a guide book for learning the current structure biology, but also those in structure biology may use this book as a comprehensive reference to cover broad topics.
Author: Per J. Kraulis
Publisher:
ISBN: 9789155424749
Category : Nuclear magnetic resonance spectroscopy
Languages : en
Pages : 47
Get Book Here
Book Description
During the last 10 years, nuclear magnetic resonance (NMR) methods have been developed to determine the three-dimensional structures of small proteins. This thesis reviews these methods and discusses their limitations. The assignment of the NMR spectrum of a protein is a necessary, bbut not sufficient, requirement for the determinetion of its structure by NMR methods.For this purpose, an interactive graphics program ANSIG has been developed. Spectrum contours can be viewed, and cross peaks extracted, connested and assigned. Assignment lists and distance restraints lists derived from NOESY cross peak intensities can be produced. A novel algorithm for the calculation of three-dimensional protein structures from inter-proton distance restraints derived ,from NMR data has been implemented in the program NOEDIA. A data base of crystallographically determined protein structured is searched for fragments that fit the data and the protein structure is built from them. Cecropin A, an antibacterial peptide from the giant silk moth HYALOPHORA CECROPIA, was investigated in a solution containing 15% hexafluoroisopropyl alchohol. The three-dimensional structure of the 37-residue peptide was computed from the NMR data ba the simulated dynamical annealing method. It consists of two amphiphatic a-helical regions 5-21 and 24-37, whose relative orientation could not be defined. The cellulases of the filamentous fungus TRICHODERMA REESEI contain a strongly conserved small terminal domain, which is the N-terminal in two of them and C-terminal in the other two. The structure of a synthetic 36-residue peptide corresponding to the C-terminal domain of cellobiohydrolase I was determined grom NMR data using a hybrid distance geometry-dynamic simulated annealing method. The molecule is wedge-shaped and consists of an irregular triplestranded anti-parallel B-sheet. The structure is very well defined due to extensive stereospecific assignments and permits analysis of a number of side chain interactions. The first example of a 3D NMR spectrum of a protein is presented. The combination of two 2D experiments into a 3D experiment facilitates cross peak assignment and potentially increases the size of proteins amenable to structure determination ba NMR.
Author: Julia Koehler Leman
Publisher:
ISBN:
Category : Electronic dissertations
Languages : en
Pages : 315
Get Book Here
Book Description
Author: Arash Bahrami
Publisher:
ISBN:
Category :
Languages : en
Pages : 190
Get Book Here
Book Description
Author: Massimiliano Bonomi
Publisher: Frontiers Media SA
ISBN: 2889740897
Category : Science
Languages : en
Pages : 182
Get Book Here
Book Description
