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Author: T.J.R. Harris
Publisher: Springer
ISBN: 1461315654
Category : Science
Languages : en
Pages : 250
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Book Description
There are very few parts of biology that remain free from the influence of Genetic Engineering developed in the early 1970s. Disciplines as wide apart as Brewing, Forensic Science and Population Genetics have all been affected in some way. The major impact, however, has been to create a new science of Biotechnology - a part of which is the production of proteins in a variety of cellular systems. Initially, bacterial systems such as E. coli were used but it soon became apparent that this prokaryotic host was not suitable for the preparation of more complicated proteins. In December 1988, a Symposium sponsored by the Biological Council organised by Dr Chris Hentschel and myself was held at the Middlesex in London to discuss alternative methods of Hospital Medical School protein production and to review some ofthe applications of the proteins so produced. The presentations at this meeting form the substance ofthis book. The theme is apparent from the first part where the expression of proteins and their domains in yeast is described and compared to other fungal and bacterial systems, such as Aspergillus and Bacillus subtilis. The successful use of recombinant yeast to produce hepatitis B surface antigen for vaccine purposes is particularly pertinent.
Author: T.J.R. Harris
Publisher: Springer
ISBN: 1461315654
Category : Science
Languages : en
Pages : 250
Get Book Here
Book Description
There are very few parts of biology that remain free from the influence of Genetic Engineering developed in the early 1970s. Disciplines as wide apart as Brewing, Forensic Science and Population Genetics have all been affected in some way. The major impact, however, has been to create a new science of Biotechnology - a part of which is the production of proteins in a variety of cellular systems. Initially, bacterial systems such as E. coli were used but it soon became apparent that this prokaryotic host was not suitable for the preparation of more complicated proteins. In December 1988, a Symposium sponsored by the Biological Council organised by Dr Chris Hentschel and myself was held at the Middlesex in London to discuss alternative methods of Hospital Medical School protein production and to review some ofthe applications of the proteins so produced. The presentations at this meeting form the substance ofthis book. The theme is apparent from the first part where the expression of proteins and their domains in yeast is described and compared to other fungal and bacterial systems, such as Aspergillus and Bacillus subtilis. The successful use of recombinant yeast to produce hepatitis B surface antigen for vaccine purposes is particularly pertinent.
Author: Otto-Wilhelm Merten
Publisher: Springer Science & Business Media
ISBN: 9780792371373
Category : Medical
Languages : en
Pages : 434
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Book Description
The general field of fundamental and applied biotechnology becomes increasingly important for the production of biologicals for human and veterinary use, by using prokaryotic and eukaryotic microorganisms. The papers in the present book are refereed articles compiled from oral and poster presentations from the EFB Meeting on Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology, which was organized in Semmering/A from 5th to 8th October 2000. A special feature of this meeting was the comparison of different classes of host cells, mainly bacteria, yeasts, filamentous fungi, and animal cells, which made obvious that many physiological features of recombinant protein formation, like cell nutrition, stress responses, protein folding and secretion, or genetic stability, follow similar patterns in different expression systems. This comparative aspect is by far the point of most interest because such comparisons are rarely done, and if they are done, their results are most often kept secret by the companies who generated them. Audience: Presently, a comparable book does not exist because the compiling of manuscripts from all fields of biotechnology (prokaryotic as well as eukaryotic, up to animal cell biotechnology) is not done in general. This particularity makes this book very interesting for postgraduate students and professionals in the large field of biotechnology who want to get a more global view on the current state of the expression of recombinant biologicals in different host cell systems, the physiological problems associated with the use of different expression systems, potential approaches to solve such difficulties by metabolic engineering or the use of other host cells, and the cooperation between process development and strain improvement, which is crucial for the optimisation of both the production strain and the process. This book should be in every library of an institution/organization involved in biotechnology.
Author: Vijai K. Pasupuleti
Publisher: Springer Science & Business Media
ISBN: 1402066740
Category : Science
Languages : en
Pages : 237
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Book Description
Protein hydrolysates, otherwise commonly known as peptones or peptides, are used in a wide variety of products in fermentation and biotechnology industries. The term “peptone” was first introduced in 1880 by Nagelli for growing bacterial cultures. However, later it was discovered that peptones derived from the partial digestion of proteins would furnish organic nitrogen in readily available form. Ever since, p- tones, which are commonly known as protein hydrolysates, have been used not only for growth of microbial cultures, but also as nitrogen source in commercial fermen- tions using animal cells and recombinant microorganisms for the production of value added products such as therapeutic proteins, hormones, vaccines, etc. Today, the characterization, screening and manufacturing of protein hyd- lysates has become more sophisticated, with the introduction of reliable analytical instrumentation, high throughput screening techniques coupled with statistical design approaches, novel enzymes and efficient downstream processing equipment. This has enabled the introduction of custom-built products for specialized appli- tions in diverse fields of fermentation and biotechnology, such as the following. 1. Protein hydrolysates are used as much more than a simple nitrogen source. For example, the productivities of several therapeutic drugs made by animal cells and recombinant microorganisms have been markedly increased by use of p- tein hydrolysates. This is extremely important when capacities are limited. 2. Protein hydrolysates are employed in the manufacturing of vaccines by ferm- tation processes and also used as vaccine stabilizers.
Author: Otto-Wilhelm Merten
Publisher: Springer Science & Business Media
ISBN: 9401597499
Category : Science
Languages : en
Pages : 396
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Book Description
More then 20 years have passed now since the first recombinant protein producing microorganisms have been developed. In the meanwhile, numerous proteins have been produced in bacteria, yeasts and filamentous fungi, as weIl as higher eukaryotic cells, and even entire plants and animals. Many recombinant proteins are on the market today, and some of them reached substantial market volumes. On the first sight one would expect the technology - including the physiology of the host strains - to be optimised in detail after a 20 year's period of development. However, several constraints have limited the incentive for optimisation, especially in the pharmaceutical industry like the urge to proceed quickly or the requirement to define the production parameters for registration early in the development phase. The additional expenses for registration of a new production strain often prohibits a change to an optimised strain. A continuous optimisation of the entire production process is not feasible for the same reasons.
Author: Hansjörg Hauser
Publisher: Walter de Gruyter
ISBN: 3110809281
Category : Science
Languages : en
Pages : 513
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Book Description
„Hauser und Wagner haben die neuen Möglichkeiten der Mammalian Cell Biology sehr anregend dargestellt.“ Prof. Dr. Hans Fritz, Ludwig-Maximilians-Universität München
Author: John A. Howard
Publisher: Springer
ISBN: 3662438364
Category : Science
Languages : en
Pages : 282
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Book Description
Attention has recently turned to using plants as hosts for the production of commercially important proteins. The twelve case studies in this volume present successful strategies for using plants to produce industrial and pharmaceutical proteins and vaccine antigens. They examine in detail projects that have commercial potential or products that have already been commercialized, illustrating the advantages that plants offer over bacterial, fungal or animal cell-culture hosts. There are many indications that plant protein production marks the beginning of a new paradigm for the commercial production of proteins that, over the next decade, will expand dramatically.
Author: Elizabeth E. Hood
Publisher: Springer Science & Business Media
ISBN: 9401726930
Category : Medical
Languages : en
Pages : 232
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Book Description
This exciting volume Plants as Factories for Protein Production, edited by Drs. Elizabeth E. Hood and John A. Howard, contains chapters by experts in the field of molecular farming. The information within addresses the leading plant systems for recombinant protein production, as well as the progress being made in leading product categories - human pharmaceuticals, animal health, and industrial enzymes. More importantly, the book includes chapters that address the hot topics of production, containment, regulatory, and legal aspects that are quickly coming to the forefront of the industry. This most timely text is appropriate for graduate students and post-doctoral fellows, as well as being a key text for faculty, pharmaceutical producers, and industrial enzyme users.
Author: Varsha Gupta
Publisher: Springer
ISBN: 9811008752
Category : Technology & Engineering
Languages : en
Pages : 543
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Book Description
This book explores the journey of biotechnology, searching for new avenues and noting the impressive accomplishments to date. It has harmonious blend of facts, applications and new ideas. Fast-paced biotechnologies are broadly applied and are being continuously explored in areas like the environmental, industrial, agricultural and medical sciences. The sequencing of the human genome has opened new therapeutic opportunities and enriched the field of medical biotechnology while analysis of biomolecules using proteomics and microarray technologies along with the simultaneous discovery and development of new modes of detection are paving the way for ever-faster and more reliable diagnostic methods. Life-saving bio-pharmaceuticals are being churned out at an amazing rate, and the unraveling of biological processes has facilitated drug designing and discovery processes. Advances in regenerative medical technologies (stem cell therapy, tissue engineering, and gene therapy) look extremely promising, transcending the limitations of all existing fields and opening new dimensions for characterizing and combating diseases.
Author: Gyun Min Lee
Publisher: John Wiley & Sons
ISBN: 3527343342
Category : Science
Languages : en
Pages : 436
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Book Description
Offers a comprehensive overview of cell culture engineering, providing insight into cell engineering, systems biology approaches and processing technology In Cell Culture Engineering: Recombinant Protein Production, editors Gyun Min Lee and Helene Faustrup Kildegaard assemble top class authors to present expert coverage of topics such as: cell line development for therapeutic protein production; development of a transient gene expression upstream platform; and CHO synthetic biology. They provide readers with everything they need to know about enhancing product and bioprocess attributes using genome-scale models of CHO metabolism; omics data and mammalian systems biotechnology; perfusion culture; and much more. This all-new, up-to-date reference covers all of the important aspects of cell culture engineering, including cell engineering, system biology approaches, and processing technology. It describes the challenges in cell line development and cell engineering, e.g. via gene editing tools like CRISPR/Cas9 and with the aim to engineer glycosylation patterns. Furthermore, it gives an overview about synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production. In addition, the book discusses the most important aspects of production processes, including cell culture media, batch, fed-batch, and perfusion processes as well as process analytical technology, quality by design, and scale down models. -Covers key elements of cell culture engineering applied to the production of recombinant proteins for therapeutic use -Focuses on mammalian and animal cells to help highlight synthetic and systems biology approaches to cell culture engineering, exemplified by the widely used CHO cell line -Part of the renowned "Advanced Biotechnology" book series Cell Culture Engineering: Recombinant Protein Production will appeal to biotechnologists, bioengineers, life scientists, chemical engineers, and PhD students in the life sciences.
Author: Brigitte Gasser
Publisher:
ISBN: 9781493990245
Category : Recombinant proteins
Languages : en
Pages : 431
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Book Description
