PRINS and PNA Technologies in Chromosomal Investigations PDF Download
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Author: Franck Pellestor
Publisher: Nova Publishers
ISBN: 9781600214134
Category : Science
Languages : en
Pages : 208
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Book Description
Book & CD. Advances in molecular biotechnology have greatly improved the sensitivity and the efficiency of methods utilised for genetic investigations and diagnosis. In the domain of chromosome analysis, the introduction of molecular techniques has led to the development of a new approach, called Molecular Cytogenetics, which has surpassed previously available techniques to become a foremost biological method. The fluorescence in situ hybridisation (FISH) is quickly became the standard technique for in situ chromosomal investigations, as illustrated by its large variety of applications in research and diagnosis. However, during the last decade, alternative methods to FISH have been introduced and have shown to be valuable in detecting chromosomes and quantifying chromosomal abnormalities. These alternative procedures are the Primed IN Situ (PRINS) labelling and the Peptide Nucleic Acid (PNA) probes. The two procedures present several advantages for the in situ detection of nucleic acid sequences, such as the small size of PNA probes and PRINS primers, or the fast kinetics of PRINS and PNA labelling reactions, that make them very attractive for a number of cytogenetic purposes. This book provides a valuable introduction and overview of the principles and the applications of alternative approaches in the field of molecular cytogenetics.
Author: Franck Pellestor
Publisher: Nova Publishers
ISBN: 9781600214134
Category : Science
Languages : en
Pages : 208
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Book Description
Book & CD. Advances in molecular biotechnology have greatly improved the sensitivity and the efficiency of methods utilised for genetic investigations and diagnosis. In the domain of chromosome analysis, the introduction of molecular techniques has led to the development of a new approach, called Molecular Cytogenetics, which has surpassed previously available techniques to become a foremost biological method. The fluorescence in situ hybridisation (FISH) is quickly became the standard technique for in situ chromosomal investigations, as illustrated by its large variety of applications in research and diagnosis. However, during the last decade, alternative methods to FISH have been introduced and have shown to be valuable in detecting chromosomes and quantifying chromosomal abnormalities. These alternative procedures are the Primed IN Situ (PRINS) labelling and the Peptide Nucleic Acid (PNA) probes. The two procedures present several advantages for the in situ detection of nucleic acid sequences, such as the small size of PNA probes and PRINS primers, or the fast kinetics of PRINS and PNA labelling reactions, that make them very attractive for a number of cytogenetic purposes. This book provides a valuable introduction and overview of the principles and the applications of alternative approaches in the field of molecular cytogenetics.
Author: Franck Pellestor
Publisher: Springer Science & Business Media
ISBN: 1597450685
Category : Science
Languages : en
Pages : 257
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Book Description
The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen increasing applications in these fields. By combining the high sensitivity of the PCR reaction and the cytological localization of target sequences, both PRINS and in situ PCR techniques have provided highly powerful complements to FISH for in situ cellular and molecular investigations. Both these approaches have several advantages in terms of sensitivity and specificity, owing to the use of primers and to the fast kinetics of annealing and elongation reactions in situ. In the first edition of PRINS and In Situ PCR Protocols edited by John R. Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in a wide range of clinical conditions. Since the publication of this successful reference book, there have been s- nificant improvements in in situ detection techniques. This completely revised and updated second edition presents a compreh- sive selection of new procedures developed in the field of PRINS and in situ PCR technologies. The book has two sections. Part I, Basic Methodology, contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocols for PRINS detection of unique sequences in situ.
Author: Jan Trøst Jørgensen
Publisher: Academic Press
ISBN: 0128135409
Category : Medical
Languages : en
Pages : 508
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Book Description
Companion and Complementary Diagnostics: From Biomarker Discovery to Clinical Implementation provides readers with in-depth insights into the individual steps in the development of companion diagnostic assays, from the early biomarker discovery phase straight through to final regulatory approval. Further, the clinical implementation of companion diagnostic testing in the clinic is also discussed. As the development of predictive or selective biomarker assays linked to specific drugs is substantially increasing, this book offers comprehensive information on this quickly-evolving area of biomedicine. It is an essential resource for those in academic institutions, hospitals and pharma, and biotech and diagnostic commercial companies. Covers all aspects, from biomarker discovery, to development and regulatory approval Explains the "how to" aspects of companion diagnostics Incorporates information on the entire process, allowing for easier and deeper understanding of the topic
Author:
Publisher:
ISBN:
Category : American literature
Languages : en
Pages : 1132
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Book Description
Author: Thomas Liehr
Publisher: Springer Science & Business Media
ISBN: 3540705813
Category : Science
Languages : en
Pages : 447
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Book Description
This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.
Author: Bernd W. Rautenstrauß
Publisher: Elsevier
ISBN: 9783540672760
Category : Medical
Languages : en
Pages : 508
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Book Description
Fluorescence in situ hybridization (FISH) has been developed as a powerful technology which allows direct visualisation or localisation of genomic alterations. The technique has been adopted to a range of applications in both medicine, especially in the areas of diagnostic cytogenetics, and biology. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, such as formalin fixed and paraffin embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and xpression of apoptosis-related genes; comparative genomic ybridization; and special FISH techniques.
Author:
Publisher:
ISBN:
Category : Polycyclic aromatic hydrocarbons
Languages : en
Pages : 500
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Book Description
Author: Thomas Liehr
Publisher: Academic Press
ISBN: 0128235802
Category : Science
Languages : en
Pages : 430
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Book Description
Cytogenomics demonstrates that chromosomes are crucial in understanding the human genome and that new high-throughput approaches are central to advancing cytogenetics in the 21st century. After an introduction to (molecular) cytogenetics, being the basic of all cytogenomic research, this book highlights the strengths and newfound advantages of cytogenomic research methods and technologies, enabling researchers to jump-start their own projects and more effectively gather and interpret chromosomal data. Methods discussed include banding and molecular cytogenetics, molecular combing, molecular karyotyping, next-generation sequencing, epigenetic study approaches, optical mapping/karyomapping, and CRISPR-cas9 applications for cytogenomics. The book’s second half demonstrates recent applications of cytogenomic techniques, such as characterizing 3D chromosome structure across different tissue types and insights into multilayer organization of chromosomes, role of repetitive elements and noncoding RNAs in human genome, studies in topologically associated domains, interchromosomal interactions, and chromoanagenesis. This book is an important reference source for researchers, students, basic and translational scientists, and clinicians in the areas of human genetics, genomics, reproductive medicine, gynecology, obstetrics, internal medicine, oncology, bioinformatics, medical genetics, and prenatal testing, as well as genetic counselors, clinical laboratory geneticists, bioethicists, and fertility specialists. Offers applied approaches empowering a new generation of cytogenomic research using a balanced combination of classical and advanced technologies Provides a framework for interpreting chromosome structure and how this affects the functioning of the genome in health and disease Features chapter contributions from international leaders in the field
Author:
Publisher: Academic Press
ISBN: 0080984525
Category : Science
Languages : en
Pages : 331
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Book Description
This is the first book to be devoted entirely to the application and development of flow techniques in cytogenetics. It provides comprehensive information on the use of flow cytometry and sorting for chromosome classification and purification. Cytogenetics and molecular biologists will find this book an invaluable reference source. Practical details for the preparation and analysis of chromosomes using flow cytometry Flow karyotyping for sensitive rapid analysis of chromosome normality and the detection of aberrant chromosomes Flow sorting as a source of chromosome-specific DNA for gene mapping and recombinant DNA libraries Construction and current status of chromosome-specific recombinant DNA libraries
Author: Nils Stein
Publisher: Springer
ISBN: 3319925288
Category : Science
Languages : en
Pages : 400
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Book Description
This book presents an overview of the state-of-the-art in barley genome analysis, covering all aspects of sequencing the genome and translating this important information into new knowledge in basic and applied crop plant biology and new tools for research and crop improvement. Unlimited access to a high-quality reference sequence is removing one of the major constraints in basic and applied research. This book summarizes the advanced knowledge of the composition of the barley genome, its genes and the much larger non-coding part of the genome, and how this information facilitates studying the specific characteristics of barley. One of the oldest domesticated crops, barley is the small grain cereal species that is best adapted to the highest altitudes and latitudes, and it exhibits the greatest tolerance to most abiotic stresses. With comprehensive access to the genome sequence, barley’s importance as a genetic model in comparative studies on crop species like wheat, rye, oats and even rice is likely to increase.
