Author: Jonathan M. Gibbins
Publisher: Springer Science & Business Media
ISBN: 1592597823
Category : Medical
Languages : en
Pages : 389
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Book Description
12 The average human body has in the order of 10 circulating platelets. They are crucial for hemostasis, and yet excessive platelet activation is a major cause of m- bidity and mortality in western societies. It is therefore not surprising that platelets have become one of the most extensively investigated biological cell types. We are, however, far from understanding precisely how platelets become activated under physiological and pathophysiological conditions. In addition, there are large gaps in our knowledge of platelet production from their giant precursor cell, the megakar- cyte. Understanding megakaryocyte biology will be crucial for the development of platelet gene targeting. The aim of Platelets and Megakaryocytes is therefore to bring together established and recently developed techniques to provide a comprehensive guide to the study of both the platelet and the megakaryocyte. It consists of five s- tions split between two volumes. The more functional assays appear in Volume 1, whereas Volume 2 includes signaling techniques, postgenomic methods, and a n- ber of key perspectives chapters. Part I of Volume 1, Platelets and Megakaryocytes: Functional Assays, describes many well established approaches to the study of platelet function, including aggregometry, secretion, arachidonic acid metabolism, procoagulant responses, pla- let adhesion under static or flow conditions, flow cytometry, and production of microparticles. Although one would ideally wish to perform experiments with human platelets, studies within the circulation using intravital microscopy require the use of animal models, which are described in Chapter 16, vol. 1.
Author: Jonathan M. Gibbins
Publisher: Humana Press
ISBN: 9781493985845
Category : Science
Languages : en
Pages : 287
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Book Description
The main aim of this fourth volume is to complement the first three volumes published in 2004 and 2012 by adding advanced methodologies and perspectives. Chapters guide readers through new techniques into the study of platelets and megakaryocytes, including new imaging approaches, new methods for platelet production in vitro, and systems biology approaches. Written in the highly successful Methods in Molecular Biology series format, methods chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting or avoiding known pitfalls. Authoritative and up-to-date, Platelets and Megakaryocytes: Volume 4, Additional Protocols and Perspectives adds a wealth of new expertise for the labs of scientists working in this key biological area of study.
Author: Margit Pavelka
Publisher: Springer Science & Business Media
ISBN: 3211993908
Category : Science
Languages : en
Pages : 366
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Book Description
The period between 1950 and 1980 were the golden unique insights into how pathological processes affect years of transmission electron microscopy and produced cell organization. a plethora of new information on the structure of cells This information is vital to current work in which that was coupled to and followed by biochemical and the emphasis is on integrating approaches from functional studies. TEM was king and each micrograph proteomics, molecular biology, genetics, genomics, of a new object produced new information that led to molecular imaging and physiology and pathology to novel insights on cell and tissue organization and their understand cell functions and derangements in disease. functions. The quality of data represented by the images In this current era, there is a growing tendency to of cell and tissues had been perfected to a very high level substitut e modern light microscopic techniques for by the great microscopists of that era including Palade, electron microscopy, because it is less technically Porter, Fawcett, Sjostrand, Rhodin and many others. At demanding and is more readily available to researchers- present, the images that we see in leading journals for This atlas reminds us that the information obtained by the most part do not reach the same technical level and electron microscopy is invaluable and has no substitute.
Author: Harald Schulze
Publisher: Springer
ISBN: 9783319819143
Category : Medical
Languages : en
Pages : 0
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Book Description
This book gives a comprehensive insight into platelet biogenesis, platelet signal transduction, involvement of platelets in disease, the use of diverse animal models for platelet research and future perspectives in regard to platelet production and gene therapy. Being written by international experts, the book is a concise state-of-the art work in the field of platelet biogenesis, biology and research. It represents an indispensable tool for research scientists in biomedicine, vascular biology, hematopoiesis and hemostasis and specifically for scientists in platelet research, as well as for clinicians in the field of hematology and transfusion medicine.
Author: Gilles Lugassy
Publisher: CRC Press
ISBN: 1135411891
Category : Medical
Languages : en
Pages : 241
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Book Description
This text provides comprehensive and timely coverage of the current knowledge of cancer-associated thrombosis, its pathogenesis, clinical features, prevention, and therapy. It specifically addresses the relationship between hemostatic systems and cancer, thus providing a unique and much needed focus. All of the contributors are acknowledged specialists in their fields and have experience conducting large clinical trials in oncology and thrombosis. Their discussions cover all aspects of the topic, from long-term complications to cancer surgery. It will be of interest to general practitioners, internists, oncologists, hematologists, and all physicians involved in the management of cancer patients.
Author: Jonathan M. Gibbins
Publisher: Springer Science & Business Media
ISBN: 9781588291011
Category : Medical
Languages : en
Pages : 0
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Book Description
For both volumes: A comprehensive collection of cutting-edge methods for the molecular and cellular analysis of platelets and megakaryocytes. Volume 1 details basic and advanced techniques for analyzing platelet and megakaryocyte function. The approaches presented for platelet analysis include aggregometry, secretion, arachidonic acid metabolism, procoagulent response, platelet adhesion under static and flow conditions, flow cytometry, and production of microparticles. The techniques for study of megakaryocyte function allow the measurement of specialized structures for platelet production, the appearance of platelet-specific surface receptors, and the increase in ploidy. Volume 2 offers techniques for studying many aspects of signaling in platelets and megakaryocytes, as well as state-of-the-art commentaries on our understanding of these cells. The methods include ligand-binding assays, the study of protein and lipid kinases and phosphatases, the analysis of lipid rafts, and the measurement of intracellular calcium levels. There are also techniques for study of electrophysiological events, nitric oxide signaling, venom proteins, and for the internalization of proteins into platelets through permeabilization. This volume also describes the application of molecular and post-genomic methods for the study of platelets and megakaryocytes. All protocols follow the successful Methods in Molecular Biology⢠series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. The two volumes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.
Author: David Kuter
Publisher: Springer Science & Business Media
ISBN: 1461239583
Category : Medical
Languages : en
Pages : 411
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Book Description
David Kuter and a host of leading international researchers summarize in one volume all the knowledge of thrombopoietins (TPO) available today. The distinguished experts review the history of the search to discover TPO, describe the molecular and biological characteristics of this new molecule, and present the results of the preclinical animal experiments that will guide clinical use of this new hormone. Along the way they provide the most recent and comprehensive guide to the biology of megakaryocytes and platelets.
Author: Pedro A. de Alarcón
Publisher: Cambridge University Press
ISBN: 1108488986
Category : Medical
Languages : en
Pages : 501
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Book Description
An essential guide to the pathogenesis, diagnosis and management of hematologic problems in the neonate, covering erythrocyte disorders, leukocyte disorders, immunologic disorders and hemostatic disorders. Guidance is practical, including blood test interpretation, advice on transfusions and reference ranges for hematological values.
Author: Steve W. Kerrigan
Publisher: BoD – Books on Demand
ISBN: 9535122088
Category : Medical
Languages : en
Pages : 250
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Book Description
Platelets play a key role in thrombosis and haemostasis. However recent evidence clearly demonstrates that the functional role of platelets extends to many other processes in the body. With an internationally recognised list of contributing authors, The Non-Thrombotic Role of Platelets in Health and Disease, is a unique and definitive source of state-of-the-art knowledge about the additional role of platelets outside thrombosis and haemostasis. The intended audience for The Non-Thrombotic Role of Platelets in Health and Disease includes platelet biologists, microbiologists, immunologists, haematologists, oncologists, respiratory physicians, cardiologists, neurobiologists, tissue engineers, as well as students and fellows in these areas.
Author: Kenneth Kaushansky
Publisher: McGraw Hill Professional
ISBN: 126011709X
Category : Medical
Languages : en
Pages : 545
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Book Description
Hemostasis and Thrombosis as only Williams can cover them A Doody’s Core Title for 2019! Featuring content derived from Williams Hematology, Ninth Edition. this concise, full-color resource delivers comprehensive and current coverage of hemostasis and thrombosis, and platelet and megakaryocyte disorders. More than a sectional reprint, the book includes updated science and treatment recommendations not found in the 2015 release of Williams Hematology, Ninth Edition. Perfect for use at the point of care, Williams Hemostasis and Thrombosis offers the most current evaluation and treatment options for patients with bleeding and thrombotic disorders. •Covers the latest advances in hemostasis and thrombosis •A handy quick summary appears at the beginning of each chapter •Discusses the physiologic basis for hemostasis and thrombosis
