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Author: Stefan de Folter
Publisher: Humana Press
ISBN: 9781493990412
Category : Science
Languages : en
Pages : 363
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Book Description
This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs, bioinformatic analyses, and strategies for functional analyses of miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant MicroRNAs: Method and Protocols aims to ensure successful results in the further study of this vital area of plant science.
Author: Stefan de Folter
Publisher: Humana Press
ISBN: 9781493990412
Category : Science
Languages : en
Pages : 363
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Book Description
This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs, bioinformatic analyses, and strategies for functional analyses of miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant MicroRNAs: Method and Protocols aims to ensure successful results in the further study of this vital area of plant science.
Author: Mirza Hasanuzzaman
Publisher: Springer
ISBN: 3030061183
Category : Science
Languages : en
Pages : 506
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Book Description
Plants have to manage a series of environmental stresses throughout their entire lifespan. Among these, abiotic stress is the most detrimental; one that is responsible for nearly 50% of crop yield reduction and appears to be a potential threat to global food security in coming decades. Plant growth and development reduces drastically due to adverse effects of abiotic stresses. It has been estimated that crop can exhibit only 30% of their genetic potentiality under abiotic stress condition. So, this is a fundamental need to understand the stress responses to facilitate breeders to develop stress resistant and stress tolerant cultivars along with good management practices to withstand abiotic stresses. Also, a holistic approach to understanding the molecular and biochemical interactions of plants is important to implement the knowledge of resistance mechanisms under abiotic stresses. Agronomic practices like selecting cultivars that is tolerant to wide range of climatic condition, planting date, irrigation scheduling, fertilizer management could be some of the effective short-term adaptive tools to fight against abiotic stresses. In addition, “system biology” and “omics approaches” in recent studies offer a long-term opportunity at the molecular level in dealing with abiotic stresses. The genetic approach, for example, selection and identification of major conditioning genes by linkage mapping and quantitative trait loci (QTL), production of mutant genes and transgenic introduction of novel genes, has imparted some tolerant characteristics in crop varieties from their wild ancestors. Recently research has revealed the interactions between micro-RNAs (miRNAs) and plant stress responses exposed to salinity, freezing stress and dehydration. Accordingly transgenic approaches to generate stress-tolerant plant are one of the most interesting researches to date. This book presents the recent development of agronomic and molecular approaches in conferring plant abiotic stress tolerance in an organized way. The present volume will be of great interest among research students and teaching community, and can also be used as reference material by professional researchers.
Author: Deepu Pandita
Publisher: CRC Press
ISBN: 1000919080
Category : Science
Languages : en
Pages : 584
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Book Description
MicroRNAs (miRNAs) are small (20–24 nt), single stranded, regulatory RNA molecules or gene regulators of critical transcriptional or post-transcriptional gene regulation in plants in sequence-specific order that respond to numerous abiotic stresses and animals, non-coding, highly evolutionarily conserved and widely distributed throughout the plant kingdom. MiRNAs are master regulators of plant growth and development, development attenuation under various environmental stresses by stress-responsive miRNAs and plant stress responses and tolerance. Drought, salinity, heat, cold, UV radiation, heavy metal, pathogens, pests and other microbial infections affect survival, growth, development, quality, yield, and production of plants. Stress induced miRNAs down regulate their target miRNAs. This down regulation leads to the accumulation and function of positive regulators, highlighting their roles in stress responses and tolerance. Plant miRNA mediated modifications include overexpression or repression of stress-responsive miRNAs and/or their target complementary or partially complementary gene products, miRNA-resistant target genes, target-mimics and artificial miRNAs. Thus, miRNAs may serve as "genomic gold mines", novel, potent and potential targets in plant genetic manipulations and miRNA-based biotechnology will aid plant improvement and crop-plant tolerance to different environmental stresses. This book reviews our recent understanding of plant microRNAs, biogenesis and functions, computational tools and bioinformatics, regulation of plant growth and development, expression studies, and the role of plant miRNAs in various biotic and abiotic stress-response regulation in plants.
Author: Dinesh Kumar Srivastava
Publisher: Springer Nature
ISBN: 9811623392
Category : Technology & Engineering
Languages : en
Pages : 727
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Book Description
This book caters to the need of researchers working in the ever-evolving field of agricultural biotechnology. It discusses and provides in-depth information about latest advancements happening in this field. The book discusses evolution of plant tissue culture techniques, development of doubled haploids technology, role of recombinant-DNA technology in crop improvement. It also provides an insight into the global status of genetically modified crops, use of RNAi technology and mi-RNAs in plant improvement. Chapters are also dedicated for different branches of ‘omics’ science including genomics, bioinformatics, proteomics, metabolomics and phenomics along with the use of molecular markers in tagging and mapping of various genes/QTLs of agronomic importance. This book also covers the role of enzymes and microbes in agriculture in productivity enhancement. It is of interest to teachers, researchers of biotechnology and agriculture scientists. Also the book serves as additional reading material for undergraduate and postgraduate students of biotechnology, agriculture, horticulture, forestry, ecology, soil science, and environmental sciences. National and international biotechnologists and agricultural scientists will also find this to be a useful read.
Author: Shabir H. Wani
Publisher: John Wiley & Sons
ISBN: 1119432367
Category : Science
Languages : en
Pages : 315
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Book Description
Demystifies the genetic, biochemical, physiological, and molecular mechanisms underlying heat stress tolerance in plants Heat stress—when high temperatures cause irreversible damage to plant function or development—severely impairs the growth and yield of agriculturally important crops. As the global population mounts and temperatures continue to rise, it is crucial to understand the biochemical, physiological, and molecular mechanisms of thermotolerance to develop ‘climate-smart’ crops. Heat Stress Tolerance in Plants provides a holistic, cross-disciplinary survey of the latest science in this important field. Presenting contributions from an international team of plant scientists and researchers, this text examines heat stress, its impact on crop plants, and various mechanisms to modulate tolerance levels. Topics include recent advances in molecular genetic approaches to increasing heat tolerance, the potential role of biochemical and molecular markers in screening germplasm for thermotolerance, and the use of next-generation sequencing to unravel the novel genes associated with defense and metabolite pathways. This insightful book: Places contemporary research on heat stress in plants within the context of global climate change and population growth Includes diverse analyses from physiological, biochemical, molecular, and genetic perspectives Explores various approaches to increasing heat tolerance in crops of high commercial value, such as cotton Discusses the applications of plant genomics in the development of thermotolerant ‘designer crops’ An important contribution to the field, Heat Stress Tolerance in Plants is an invaluable resource for scientists, academics, students, and researchers working in fields of pulse crop biochemistry, physiology, genetics, breeding, and biotechnology.
Author: R. Knight
Publisher: Springer Science & Business Media
ISBN: 9401143854
Category : Science
Languages : en
Pages : 694
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Book Description
Proceedings of the Third International Food Legumes Research Conference
Author: Jud G. Isebrands
Publisher: CABI
ISBN: 1780641087
Category : Technology & Engineering
Languages : en
Pages : 700
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Book Description
Poplars and willows form an important component of forestry and agricultural systems, providing a wide range of wood and non-wood products. This book synthesizes research on poplars and willows, providing a practical worldwide overview and guide to their basic characteristics, cultivation and use, issues, problems and trends. Prominence is given to environmental benefits and the importance of poplar and willow cultivation in meeting the needs of people and communities, sustainable livelihoods, land use and development.
Author: Ramanjulu Sunkar
Publisher: Humana
ISBN: 9781071639726
Category : Science
Languages : en
Pages : 0
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Book Description
This fully updated new edition explores new techniques for studying plant stress. This includes novel methodologies such as MeRIP-seq for identifying changes in m6A profiles, isolation of stress granules, and additional methodologies such as MNase–seq for identifying nucleosome occupancy, alternative splicing analysis, identifying proteins that interact with long noncoding RNAs, untargeted metabolomics, ROS and NO measurements, priming-related protocols, growth-promoting bacteria isolation and functional characterization, as well as isolating mutants for stress-regulated genes using CRISPR technology. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plant Stress Tolerance: Methods and Protocols, Third Edition provides a wide range of protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.
Author: Blake C. Meyers
Publisher: Humana Press
ISBN: 9781617796531
Category : Science
Languages : en
Pages : 0
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Book Description
MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects. In Plant MicroRNAs: Methods and Protocols, experts in the field present chapters that focus on the identification, validation, and characterization of the miRNA class of RNAs, and address important aspects about heterochromatic small interfering RNAs. In addition, the methods contained in this volume emphasize miRNA analyses, but also include ways to distinguish one class of small RNAs from another. As a volume in the highly successful Methods in Molecular BiologyTM series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Plant MicroRNAs: Methods and Protocols provides the research community with a set of protocols that will help advance vital miRNA research for all plant species, both in typical model species and non-model species alike.
Author: Praveen Guleria
Publisher: Academic Press
ISBN: 012817336X
Category : Science
Languages : en
Pages : 638
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Book Description
Plant Small RNA: Biogenesis, Regulation and Application describes the biosynthesis of small RNA in plant systems. With an emphasis on the various molecular mechanisms affected by small RNA and their applications in supporting plant growth and survival, this books presents the basics and most recent advancements in small RNA mediated plant genomics, metabolomics, proteomics and physiology. In addition, it emphasizes the various molecular mechanisms affected by small RNA and their applications in supporting plant growth and survival. Final sections cover the most recent advancements in small RNA mediated plant genomics, metabolomics, proteomics and physiology. - Presents foundational information about small RNA biology and regulation in plants - Includes small RNA pathway advances - Describes the application and scope of small RNA technology for agricultural stability
