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Author: Jozef Šamaj
Publisher: Springer Science & Business Media
ISBN: 3642324630
Category : Science
Languages : en
Pages : 337
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Book Description
Endocytosis is a fundamental cellular process by means of which cells internalize extracellular and plasma membrane cargos for recycling or degradation. It is important for the establishment and maintenance of cell polarity, subcellular signaling and uptake of nutrients into specialized cells, but also for plant cell interactions with pathogenic and symbiotic microbes. Endocytosis starts by vesicle formation at the plasma membrane and progresses through early and late endosomal compartments. In these endosomes cargo is sorted and it is either recycled back to the plasma membrane, or degraded in the lytic vacuole. This book presents an overview of our current knowledge of endocytosis in plants with a main focus on the key molecules undergoing and regulating endocytosis. It also provides up to date methodological approaches as well as principles of protein, structural lipid, sugar and microbe internalization in plant cells. The individual chapters describe clathrin-mediated and fluid-phase endocytosis, as well as flotillin-mediated endocytosis and internalization of microbes. The book was written for a broad spectrum of readers including students, teachers and researchers.
Author: Jozef Šamaj
Publisher: Springer Science & Business Media
ISBN: 3642324630
Category : Science
Languages : en
Pages : 337
Get Book Here
Book Description
Endocytosis is a fundamental cellular process by means of which cells internalize extracellular and plasma membrane cargos for recycling or degradation. It is important for the establishment and maintenance of cell polarity, subcellular signaling and uptake of nutrients into specialized cells, but also for plant cell interactions with pathogenic and symbiotic microbes. Endocytosis starts by vesicle formation at the plasma membrane and progresses through early and late endosomal compartments. In these endosomes cargo is sorted and it is either recycled back to the plasma membrane, or degraded in the lytic vacuole. This book presents an overview of our current knowledge of endocytosis in plants with a main focus on the key molecules undergoing and regulating endocytosis. It also provides up to date methodological approaches as well as principles of protein, structural lipid, sugar and microbe internalization in plant cells. The individual chapters describe clathrin-mediated and fluid-phase endocytosis, as well as flotillin-mediated endocytosis and internalization of microbes. The book was written for a broad spectrum of readers including students, teachers and researchers.
Author: Marisa S. Otegui
Publisher: Humana Press
ISBN: 9781493914210
Category : Science
Languages : en
Pages : 286
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Book Description
Plant Endosomes: Methods and Protocols explores a collection of protocols and techniques to analyze in vivo trafficking of endocytic/endosomal cargo, including lipids, fluids, proteins and ligands, ultrastructural features of endosomes by high-pressure freezing/freeze-substitution and electron tomography, as well as protein-protein interactions in the endosomal and endomembrane system. The volume continues with coverage of the sorting defects in the transport of vacuolar storage proteins, function conservation of plant endosomal proteins, endosomal trafficking during plant responses to pathogens, protein composition of endosomes and endocytic vesicles, ubiquitination of endosomal cargo proteins and the identification of novel endosomal components by chemical genomics and proteomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Detailed and practical, Plant Endosomes: Methods and Protocols gathers contributions from many leading and emerging plant membrane trafficking researchers in order to promote and facilitate novel studies and ideas in this vital field.
Author: Jozef Šamaj
Publisher: Springer
ISBN: 9783642425356
Category : Science
Languages : en
Pages : 0
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Book Description
Endocytosis is a fundamental biological process, which is conserved among all eukaryotes. It is essential not only for many physiological and signalling processes but also for interactions between eukaryotic cells and pathogens or symbionts. This book covers all aspects of endocytosis in both lower and higher plants, including basic types of endocytosis, endocytic compartments, and molecules involved in endocytic internalization and recycling in diverse plant cell types. It provides a comparison with endocytosis in animals and yeast and discusses future prospects in this new and rapidly evolving plant research field. Readers will find an overview of the state-of-the-art methods and techniques applied in plant endocytosis research.
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Book Description
Author: Jozef Šamaj
Publisher: Springer Science & Business Media
ISBN: 3642324622
Category : Science
Languages : en
Pages : 337
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Book Description
Endocytosis is a fundamental cellular process by means of which cells internalize extracellular and plasma membrane cargos for recycling or degradation. It is important for the establishment and maintenance of cell polarity, subcellular signaling and uptake of nutrients into specialized cells, but also for plant cell interactions with pathogenic and symbiotic microbes. Endocytosis starts by vesicle formation at the plasma membrane and progresses through early and late endosomal compartments. In these endosomes cargo is sorted and it is either recycled back to the plasma membrane, or degraded in the lytic vacuole. This book presents an overview of our current knowledge of endocytosis in plants with a main focus on the key molecules undergoing and regulating endocytosis. It also provides up to date methodological approaches as well as principles of protein, structural lipid, sugar and microbe internalization in plant cells. The individual chapters describe clathrin-mediated and fluid-phase endocytosis, as well as flotillin-mediated endocytosis and internalization of microbes. The book was written for a broad spectrum of readers including students, teachers and researchers.
Author: Kaija Jo Goodman
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Endocytosis and endosomal trafficking control the abundance and distribution of key membrane proteins at the cell surface and in other cellular compartments. The Endosomal Sorting Complex Required for Transport (ESCRT) mediates the formation of vesicles to sequester ubiquitinated membrane proteins (cargo) into late endosomes en route to the vacuole for degradation. We previously found that the late-acting ESCRT components, ISTL1 and LIP5 are important for plant development, proper defense responses, and multivesicular endosome formation in root cells. Using genetic, confocal, and electron microscopy approaches, I found a unique role for ISTL1 and LIP5 in endosomal trafficking in tapetal cells, which surround and nourish developing pollen grains within anthers. Together, ISTL1 and LIP5 are required in the tapetum to maintain plasma membrane localization of ABCG9, a transporter important for pollen wall development. Plants lacking ISTL1 and LIP5 showed defects in exocytosis of the sec-RFP secretion marker in anthers. Pollen in the istl1 lip5 mutant developed abnormal pollen wall patterns, leading to partial pollen lethality. Finally, istl1 lip5 tapetal cells exhibited ultrastructural defects in both multivesicular endosome morphology and trans-Golgi Networks, indicating that besides endosomal sorting, late-acting ESCRTs have a role in the early endosomal pathway and/or exocytosis. The ESCRT machinery mediates negative membrane deformation (i.e. membrane bending away from the cytoplasm). Exactly how the ESCRT proteins orchestrate membrane deformation and fission is not well known. The current models for how intralumenal vesicles form assume that vesicles are formed one at a time. However, in plants intralumenal vesicles form as concatenated networks, establishing a novel mechanism to entrap cargo more efficiently. In Chapter 3, I use electron tomography to analyze vesicle network architecture from plant endosomes. I developed quantitative tools to evaluate the complexity of individual vesicle networks and applied them to compare intralumenal vesicle networks from different cell types. In addition, I developed rules and tools to recapitulate how these intralumenal vesicle networks form and infer the activity of budding sites on the endosomal surface.
Author: Markus Geisler
Publisher: Frontiers E-books
ISBN: 2889191311
Category :
Languages : en
Pages : 148
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Book Description
Membrane proteins are essential determinants of many biological processes in plants. They function in metabolic processes, signal transduction, transport of small molecules and polymers across endo- and plasma membranes, and intercompartmental trafficking of proteins, lipids, and cell wall components. During these integrative processes, dynamic interactions of membrane proteins with other membrane or soluble components are thought to provide a high degree of flexibility that usually characterizes higher plants. This concept is supported by the recent release of a first, partial Arabidopsis interactome by the Arabidopsis Interactome Mapping Consortium (http://www.sciencemag.org/content/333/6042/601.full.htm). The Arabidopsis interactome reveals a strong enrichment of a few network communities, including those for transmembrane transport and vesicle trafficking. Strikingly, the large transmembrane transport community shares a high amount of proteins with the vesicle trafficking community suggesting a strong physical and functional overlap and interaction.
Author: Brian Ceresa
Publisher: BoD – Books on Demand
ISBN: 9535106627
Category : Science
Languages : en
Pages : 470
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Book Description
Molecular Regulation of Endocytosis is a compilation of scientific "short stories" about the entry of external substances into cells. As one can see from the chapters, endocytosis regulates diverse processes such as homeostasis of the cell, signal transduction, entry of pathogens and viruses. In addition to the experimental techniques embedded in each chapter, entire chapters are dedicated to experimental approaches that will be useful to all scientists and their model systems. For those more clinically oriented, the final chapters look to the future and ways of utilizing endocytic pathways for therapeutic purposes.
Author: Joseph Seckbach
Publisher: Springer Science & Business Media
ISBN: 9048194490
Category : Science
Languages : en
Pages : 622
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Book Description
Symbioses and Stress examines how organisms in tight symbiotic associations cope with abiotic and biotic stress. Presenting new findings on symbioses by experts and leading scholars in the field, this volume complements courses and lectures in biology and genetics.
Author: C. R. Hawes
Publisher: Cambridge University Press
ISBN: 9780521381086
Category : Medical
Languages : en
Pages : 272
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Book Description
The transport of macromolecules in membrane bounded vesicles is a fundamental process of all eukaryotic cells. The paths taken by this vesicular traffic have been intensively researched in animal cells but are less well characterised in plant cells. Nevertheless, with the development of and combination of techniques in biochemistry, molecular biology and cell biology, progress in the study of plant vesicle traffic has been achieved in recent years. This book attempts to highlight the recent advances made and to explore avenues for future research. The book opens with a general overview of vesicular traffic both in animal and plant cells. This is followed by a more detailed consideration of higher plant coated vesicles, endocytosis, exocytosis and mechanisms of vesicle traffic and transport in cells. The biochemistry, cell biology and molecular biology of each is considered and particular attention is given to those specialised cell systems in which vesicle traffic is of particular importance. This book brings together expertise from a wide range of research disciplines involved in the study of vesicle traffic in plants, and, as such, provides a unique synthesis from which the research can move forward. It will be of general interest to those involved in the study of vesicle traffic in both plant and animal cells.
