Optical Trapping and Micromanipulation of Biological Objects in Microfluidic Channels PDF Download
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Author: Marinela Gjoni Boillat
Publisher:
ISBN:
Category :
Languages : en
Pages : 121
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Book Description
Author: Marinela Gjoni Boillat
Publisher:
ISBN:
Category :
Languages : en
Pages : 121
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Book Description
Author: Michael Werner
Publisher:
ISBN:
Category :
Languages : en
Pages : 97
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Author: Ana Zehtabi-Oskuie
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
This thesis presents optical trapping of various single nanoparticles, and the method for integrating the optical trap system into a microfluidic channel to examine the trapping stiffness and to study binding at the single molecule level.Optical trapping is the capability to immobilize, move, and manipulate small objects in a gentle way. Conventional trapping methods are able to trap dielectric particles with size greater than 100 nm. Optical trapping using nanostructures has overcome this limitation so that it has been of interest to trap nanoparticles for bio-analytical studies. In particular, aperture optical trapping allows for trapping at low powers, and easy detection of the trapping events by noting abrupt jumps in the transmission intensity of the trapping beam through the aperture. Improved trapping efficiency has been achieved by changing the aperture shape from a circle; for example, to a rectangle, double nanohole (DNH), or coaxial aperture. The DNH has the advantage of a well-defined trapping region between the two cusps where the nanoholes overlap, which typically allows only single particle trapping due to steric hindrance. Trapping of 21 nm encapsulated quantum dot has been achieved which shows optical trapping can be used in technologies that seek to place a quantum dot at a specific location in a plasmonic or nanophotonic structure.The DNH has been used to trap and unfold a single protein. The high signal-to-noise ratio of 33 in monitoring single protein trapping and unfolding shows a tremendous potential for using the double nanohole as a sensor for protein binding events at a single molecule level. The DNH integrated in a microfluidic chip with flow to show that stable trapping can be achieved under reasonable flow rates of a few ?L/min. With such stable trapping under flow, it is possible to envision co-trapping of proteins to study their interactions. Co-trapping is achieved for the case where we flow in a protein (bovine serum albumin--BSA) and co-trap its antibody (anti-BSA).
Author: Jaime Castillo-León
Publisher: CRC Press
ISBN: 1439827435
Category : Medical
Languages : en
Pages : 258
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Book Description
Several micro- and nanomanipulation techniques have emerged in recent decades thanks to advances in micro- and nanofabrication. For instance, the atomic force microscope (AFM) uses a nano-sized tip to image, push, pull, cut, and indent biological material in air, liquid, or vacuum. Using micro- and nanofabrication techniques, scientists can make manipulation tools, such as microgrippers and nanotweezers, on the same length scale as the biological samples. Micro and Nano Techniques for the Handling of Biological Samples reviews the different techniques available to manipulate and integrate biological materials in a controlled manner, either by sliding them along a surface (2-D manipulation), or by gripping and moving them to a new position (3-D manipulation). The advantages and drawbacks are mentioned together with examples that reflect the state-of-the-art in manipulation techniques for biological samples. Thanks to the advances in micro- and nanomanipulation techniques, the integration of biomaterials with physical transducers has been possible, giving rise to new and highly sensitive biosensing devices. Although great progress has been made, challenges are still present. To understand the complex interactions between and inside biological samples, scientists will always be working on improving technologies to manipulate, transport, sort, and integrate samples in different environments. Balanced between simplicity for the beginner and hardcore theory for the more advanced readers, this book is the ideal launching point for sharpening the scientific tools required to address these challenges.
Author: Songlin Zhuang
Publisher: Springer Nature
ISBN: 3031334108
Category : Technology & Engineering
Languages : en
Pages : 265
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Book Description
This book offers readers a series of robotic methods for manipulating zebrafish larva, one of the most popular model vertebrates widely used in biomedical research and clinical applications. The authors leverage advanced control theories, image processing algorithms, and artificial intelligence to establish a robot-assisted automated or semi-automated zebrafish larva-targeted micromanipulation system for different experimental purposes. The methods presented are generic and can be translated to manipulate other types of biological objects, such as embryos or cells. Coverage includes topics that span the procedures of manipulating zebrafish larva, such as in-plane positioning, three-dimensional orientation, deformation-controllable immobilization, organ-targeted microinjection, whole-organism imaging, and high-throughput trajectory tracking of zebrafish larvae group movement. Robotic Micromanipulation of Zebrafish Larva is written in a simple, clear, and easy-to-read style. It is an ideal reference for academic researchers and biomedical operators. It is also a valuable resource for students learning robotics, control and system theories, image processing, artificial intelligence, and biomedical engineering.
Author: Yu Sun
Publisher: John Wiley & Sons
ISBN: 3527690220
Category : Technology & Engineering
Languages : en
Pages : 608
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Book Description
Combining robotics with nanotechnology, this ready reference summarizes the fundamentals and emerging applications in this fascinating research field. This is the first book to introduce tools specifically designed and made for manipulating micro- and nanometer-sized objects, and presents such examples as semiconductor packaging and clinical diagnostics as well as surgery. The first part discusses various topics of on-chip and device-based micro- and nanomanipulation, including the use of acoustic, magnetic, optical or dielectrophoretic fields, while surface-driven and high-speed microfluidic manipulation for biophysical applications are also covered. In the second part of the book, the main focus is on microrobotic tools. Alongside magnetic micromanipulators, bacteria and untethered, chapters also discuss silicon nano- and integrated optical tweezers. The book closes with a number of chapters on nanomanipulation using AFM and nanocoils under optical and electron microscopes. Exciting images from the tiniest robotic systems at the nano-level are used to illustrate the examples throughout the work. A must-have book for readers with a background ranging from engineering to nanotechnology.
Author: Baojun Li
Publisher: Nova Science Publishers
ISBN: 9781634859981
Category : Lasers
Languages : en
Pages : 0
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Book Description
Since its first report in 1970 by A. Ashkin, optical trapping and manipulation has been widely used in the interdisciplines of micro- and nano-photonics, biophotonics, biomedicine, etc. A conventional tool for optical trapping and manipulation is the conventional optical tweezer (COT), the core part of which is a free-space focused laser beam. However, manipulation with COTs has some limitations such as manipulation inflexibility, bulky structure, diffraction limitation for nanoparticles, and limited integration functions. The introduction of optical fiber-based optical trapping and manipulation has solved these limitations. Using optical fibers with different configurations, optical trapping and manipulation with multiple functions can be realised with high flexibility, precision, and integration. By launching laser beams with different wavelengths into the fiber, both the photothermal effect and optical force can be used for optical trapping and manipulation. For the optical force manipulation, both evanescent fields at the surface of a subwavelength optical fiber and light output from a fiber end can be used for optical manipulation. The manipulation with light output from a fiber end can be divided into dual fiber tweezers and single fiber tweezers. For single fiber tweezers, one can realise the stable trapping of single particles both in a contact and non-contact manner for further applications. In addition, single fiber tweezers can also be used for multiple particle trapping and cell assembly, which can further be used for the assembly of biophotonic components and devices. Furthermore, by placing microparticles, which act as microlens at the end facet of an optical fiber, the microlens can be served as a photonic nanojet. This fiber supported photonic nanojet can be easily used for the trapping and detection of nanoparticles and biomolecules. Optical fiber-based optical trapping and manipulation have the advantages of easy fabrication, compact configuration, flexible manipulation, easy integration, wide applicability, etc., which provides for a wide application of prospects in micro- and nano-photonics, biophotonics, and biomedicine.
Author: Yu Sun
Publisher: Springer Nature
ISBN: 3030801977
Category : Technology & Engineering
Languages : en
Pages : 422
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Book Description
This book describes the substantial progress recently made in the development of micro and nanorobotic systems, utilizing magnetic, optical, acoustic, electrical, and other actuation fields. It covers several areas of micro and nanorobotics including robotics, materials science, and biomedical engineering. Field-Driven Micro and Nanorobots for Biology and Medicine provides readers with fundamental physics at the micro and nano scales, state-of-the-art technical advances in field-driven micro and nanorobots, and applications in biological and biomedical disciplines.
Author: Yeshaiahu Fainman
Publisher: McGraw Hill Professional
ISBN: 0071601570
Category : Technology & Engineering
Languages : en
Pages : 545
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Book Description
Publisher's Note: Products purchased from Third Party sellers are not guaranteed by the publisher for quality, authenticity, or access to any online entitlements included with the product. Cutting-Edge Optofluidics Theories, Techniques, and Practices Add novel functionalities to your optical design projects by incorporating state-of-the-art microfluidic technologies and tools. Co-written by industry experts, Optofluidics: Fundamentals, Devices, and Applications covers the latest functional integration of optical devices and microfluidics, as well as automation techniques. This authoritative guide explains how to fabricate optical lab-on-a-chip devices, synthesize photonic crystals, develop solid and liquid core waveguides, use fluidic self-assembly methods, and accomplish direct microfabrication in solutions. The book includes details on developing biological sensors and arrays, handling maskless lithography, designing high-Q cavities, and working with nanoscale plasmonics. Research outcomes from the DARPA-funded Center for Optofluidics Integration are also discussed. Discover how to: Work with optofluidic sources, lenses, filters, switches, and splitters Use dielectric waveguiding devices to input, move, and manipulate fluids Integrate colloidal crystals and fibers with microfluidic systems Develop bio-inspired fluidic lens systems and aspherical lenses Deploy miniaturized dye lasers, microscopes, biosensors, and resonators Analyze microfluidic systems using flow injection and fluorescent spectroscopy Build optofluidic direct fabrication platforms for innovative microstructures Accomplish optofluidic liquid actuation and particle manipulation
Author: George K. Knopf
Publisher: CRC Press
ISBN: 1439840547
Category : Technology & Engineering
Languages : en
Pages : 666
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Book Description
In Optical Nano and Micro Actuator Technology, leading engineers, material scientists, chemists, physicists, laser scientists, and manufacturing specialists offer an in-depth, wide-ranging look at the fundamental and unique characteristics of light-driven optical actuators. They discuss how light can initiate physical movement and control a variety of mechanisms that perform mechanical work at the micro- and nanoscale. The book begins with the scientific background necessary for understanding light-driven systems, discussing the nature of light and the interaction between light and NEMS/MEMS devices. It then covers innovative optical actuator technologies that have been developed for many applications. The book examines photoresponsive materials that enable the design of optically driven structures and mechanisms and describes specific light-driven technologies that permit the manipulation of micro- and nanoscale objects. It also explores applications in optofluidics, bioMEMS and biophotonics, medical device design, and micromachine control. Inspiring the next generation of scientists and engineers to advance light-driven technologies, this book gives readers a solid grounding in this emerging interdisciplinary area. It thoroughly explains the scientific language and fundamental principles, provides a holistic view of optical nano and micro actuator systems, and illustrates current and potential applications of light-driven systems.
