OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 241: The Larval Amphibian Growth and Development Assay (LAGDA) PDF Download
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Author: OECD
Publisher: OECD Publishing
ISBN: 9264242341
Category :
Languages : en
Pages : 38
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Book Description
This Test Guideline describes the Medaka Extended One Generation Test (MEOGRT), which exposes fish over multiple generations to give data relevant to ecological hazard and risk assessment of chemicals, including suspected endocrine disrupting chemicals (EDCs). Exposure in the MEOGRT starts with ...
Author: OECD
Publisher: OECD Publishing
ISBN: 9264242341
Category :
Languages : en
Pages : 38
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Book Description
This Test Guideline describes the Medaka Extended One Generation Test (MEOGRT), which exposes fish over multiple generations to give data relevant to ecological hazard and risk assessment of chemicals, including suspected endocrine disrupting chemicals (EDCs). Exposure in the MEOGRT starts with ...
Author: OECD
Publisher: OECD Publishing
ISBN: 9264076247
Category :
Languages : en
Pages : 33
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Book Description
This Test Guideline describes an amphibian metamorphosis assay intended to screen substances which may interfere with the normal functioning of the hypothalamo-pituitary-thyroid axis. The assay was validated with the species Xenopus laevis, which is ...
Author: OECD
Publisher: OECD Publishing
ISBN: 9264916784
Category :
Languages : en
Pages :
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Book Description
The purpose of the test is to measure the capacity of a chemical to activate or inhibit the transcription of a genetic construct (THb/ZIP-GFP X. laevis eleutheroembryos) either by binding to the thyroid hormone receptor or by modifying the amount of thyroid hormone available for transcription. The test is based on the use of a transgenic frog (possessing the genetic construction THb/ZIP-GFP) of the species X. laevis. GFP is a fluorescent protein that can be used to affirm the presence of the substance and to follow its action. This test is based on the quantification of fluorescence, and thus of the GFP protein, throughout the eleutheroembryo exposed to a test chemical. Indeed, if the fluorescence is > 12% by including the highest concentration of the test, the test is considered positive. Organisms are exposed in chemically inert cell culture plates (6 wells). Each well contains ten organisms in solution. In a run, 20 organisms are exposed to each test concentration. Tadpoles are exposed to the test chemical at NF stage 45 for a duration of 72 hours. A positive result of the XETA test indicates in vivo that a substance induces damage to the receptor and metabolization of the thyroid hormone. A negative result indicates that, under test conditions, the test substance does not induce detectable damage to the thyroid and its functioning.
Author: Muhammed Atamanalp
Publisher: Springer Nature
ISBN: 3031566696
Category :
Languages : en
Pages : 189
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Book Description
Author: OECD
Publisher: OECD Publishing
ISBN: 9264203788
Category :
Languages : en
Pages : 24
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Book Description
The test method described in this Test Guideline, is intended to define the lethal and sub-lethal effects of chemicals on the early life stages of the species tested. The early-life stages of fish are exposed to five concentrations of the test ...
Author: OECD
Publisher: OECD Publishing
ISBN: 9264714138
Category :
Languages : en
Pages : 50
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Book Description
The RTgill-W1 cell line assay describes a 24-well plate format fish cell line acute toxicity test using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. After 24 h of exposure to the test chemical, cell viability is assessed based on three fluorescent cell viability indicator dyes, measured on the same set of cells. Resazurin enters the cells in its non-fluorescent form and is converted to the fluorescent product, resorufin, by mitochondrial, microsomal or cytoplasmic oxidoreductases.
Author: OECD
Publisher: OECD Publishing
ISBN: 9264016198
Category :
Languages : en
Pages : 22
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Book Description
This Test Guideline is designed to assess the toxicity of substances to freshwater aquatic plants of the genus Lemna (duckweed). Exponentially growing plant cultures of the genus Lemna (Lemna gibba and Lemna minor usually) are allowed to grow as ...
Author: OECD
Publisher: OECD Publishing
ISBN: 9264264515
Category :
Languages : en
Pages : 28
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Book Description
This Test Guideline describes procedures designed to estimate the acute oral toxicity of substances to birds, and it provides three testing options: (1) limit dose test, (2) LD50-slope test, and (3) LD50-only test. The LD50-slope and LD50-only options are sequential testing procedures.
Author: OECD
Publisher: OECD Publishing
ISBN: 9264264604
Category :
Languages : en
Pages : 22
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Book Description
This Test Guideline is designed for assessing the effects of chemicals on the reproduction of collembolans in soil.
Author: OECD
Publisher: OECD Publishing
ISBN: 9264203729
Category :
Languages : en
Pages : 10
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Book Description
This Test Guideline (TG) describes a honey bee brood acute toxicity test under laboratory conditions. The method aims at the determination of the lethal dose (72-h LD50) following single exposure of larvae to a chemical. On day 1 (D1) of the study ...
