Nucleic Acid Blotting PDF Download
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Author: D C Darling
Publisher: Garland Science
ISBN: 1000142779
Category : Science
Languages : en
Pages : 124
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Book Description
Discusses very widely-used techniques. Aimed specifically at the newcomer. Provides detailed explanations unavailable elsewhere. Points out pitfalls and provides solutions. Invaluable help for experienced laboratories training newcomers.
Author: D C Darling
Publisher: Garland Science
ISBN: 1000142779
Category : Science
Languages : en
Pages : 124
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Book Description
Discusses very widely-used techniques. Aimed specifically at the newcomer. Provides detailed explanations unavailable elsewhere. Points out pitfalls and provides solutions. Invaluable help for experienced laboratories training newcomers.
Author: William B. Coleman
Publisher: Springer Science & Business Media
ISBN: 1592599281
Category : Medical
Languages : en
Pages : 593
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Book Description
Accompanying CD-ROM contains ... "a companion eBook version of Molecular diagnostics : for the clinical laboratorian, Second edition ... for downloading and use in the reader's PC or PDA."--Page 4 of cover.
Author: Robert C. Bast, Jr.
Publisher: John Wiley & Sons
ISBN: 111900084X
Category : Medical
Languages : en
Pages : 2004
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Book Description
Holland-Frei Cancer Medicine, Ninth Edition, offers a balanced view of the most current knowledge of cancer science and clinical oncology practice. This all-new edition is the consummate reference source for medical oncologists, radiation oncologists, internists, surgical oncologists, and others who treat cancer patients. A translational perspective throughout, integrating cancer biology with cancer management providing an in depth understanding of the disease An emphasis on multidisciplinary, research-driven patient care to improve outcomes and optimal use of all appropriate therapies Cutting-edge coverage of personalized cancer care, including molecular diagnostics and therapeutics Concise, readable, clinically relevant text with algorithms, guidelines and insight into the use of both conventional and novel drugs Includes free access to the Wiley Digital Edition providing search across the book, the full reference list with web links, illustrations and photographs, and post-publication updates
Author: Susan J. Karcher
Publisher: Academic Press
ISBN: 0080536832
Category : Science
Languages : en
Pages : 303
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Book Description
This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. Includes a glossary of over 200 terms important for understanding molecular biology Uses an inexpensive source of eukaryotic cells - great for schools on a budget Includes Methods Locator that provides instant access to the latest methods Contain clearly written, easy-to-follow, student-tested instructions: Sterile techniques Phage titration Gel electrophoresis of DNA Restriction enzyme digestion Plasmid isolation Transformation of E. Coli Recombinant DNA cloning Nick translation labeling Nonradioactive primer labelling Nonradioactive DNA detection Southern blotting Colony hybridization Purification of plant DNA RNA purification Northern blotting Purification of poly A+ RNA Polymerase chain reaction (PCR)
Author: Larry J. Kricka
Publisher: Elsevier
ISBN: 0080537669
Category : Science
Languages : en
Pages : 539
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Book Description
Since the publication of Nonistopic DNA Probe Techniques in 1992, the move away from radioactive materials for research and diagnostics has continued. This is due in part to public awareness of the hazards of radioactive waste and laws making radioactive disposal more difficult and costly and to improvement in both the sensitivity and convenience of nonisotopic techniques. Several new nonisotopic techniques have been developed and substantial improvements made to existing nonisotopic methods since 1992, and these are now included in Nonisotopic Probing, Blotting, and Sequencing. Nonisotopic Probing, Blotting, and Sequencing is an updated, expanded edition of the bestseller, Nonisotopic DNA Probe Techniques. It has been thoroughly revised to include the latest improvements in nonisotopic tagging techniques for macromolecules. Like its predecessor, it enables researchers to select the best nonisotopic method for their needs and maximize success by following its straightforward protocols. Provides strategies and detailed procedures for labeling, blotting, and probing specific nucleic acid sequences and, with this edition, protein molecules Gives protocols for nonisotopic DNA sequencing - new in this edition Gives extensive, practical information Presents background information for each method Provides expert accounts from the inventor or developer of each method Contains seven entirely new chapters Covers all major types of nonisotopic procedures for labeling and detection
Author: Ralph Rapley
Publisher: Springer
ISBN: 1592596428
Category : Science
Languages : en
Pages : 708
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Book Description
An authoritative team of investigators illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced scientists fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included. The goal is to provide established scientists and novices who are new to these techniques with a deeper understanding of the widest variety of biotechniques and their applications.
Author: Ralph Rapley
Publisher: Springer Science & Business Media
ISBN: 1592590381
Category : Science
Languages : en
Pages : 997
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Book Description
A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."
Author: P. M. Gilmartin
Publisher: Wiley-Interscience
ISBN:
Category : Medical
Languages : en
Pages : 166
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Book Description
This quick reference guide covers the fundamental techniques and practical tips needed to undertake a variety of nucleic acid hybridization methods.
Author: Dr Robin Martin
Publisher: Garland Science
ISBN: 1000101436
Category : Science
Languages : en
Pages : 190
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Book Description
Through its clear presentation of the basic concepts, Gel Electrophoresis: Nucleic Acids breaks new ground by describing the principles of the technique without resorting to complicated protocols and recipes.
Author: Chang-Hui Shen
Publisher: Elsevier
ISBN: 0323986099
Category : Science
Languages : en
Pages : 590
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Book Description
Diagnostic Molecular Biology, Second Edition describes the fundamentals of molecular biology in a clear, concise manner with each technique explained within its conceptual framework and current applications of clinical laboratory techniques comprehensively covered. This targeted approach covers the principles of molecular biology, including basic knowledge of nucleic acids, proteins and chromosomes; the basic techniques and instrumentations commonly used in the field of molecular biology, including detailed procedures and explanations; and the applications of the principles and techniques currently employed in the clinical laboratory. Topics such as whole exome sequencing, whole genome sequencing, RNA-seq, and ChIP-seq round out the discussion. Fully updated, this new edition adds recent advances in the detection of respiratory virus infections in humans, like influenza, RSV, hAdV, hRV but also corona. This book expands the discussion on NGS application and its role in future precision medicine. Provides explanations on how techniques are used to diagnosis at the molecular level Explains how to use information technology to communicate and assess results in the lab Enhances our understanding of fundamental molecular biology and places techniques in context Places protocols into context with practical applications Includes extra chapters on respiratory viruses (Corona)
